FastQCFastQC Report
Wed 25 May 2016
SRR2088145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643401
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT382831.4482479200091094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT377301.4273279006855184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT288061.0897325074780557No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG205740.7783155109648516No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149500.5655592927444606No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT116190.4395473861135711No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA113200.4282362002586819No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT104860.3966859360346765No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA97710.369637448120811No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA90290.3415675487752331No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG88970.33657398177575026No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT74030.2800558825543306No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT69650.2634863193287738No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA58340.22070052935593198No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA55680.2106377352509135No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA52470.1984942882294438No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT47900.18120595399638573No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46640.17643936731506116No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA42110.1593023532941086No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC41070.15536802777936454No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG39690.15014748046172335No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38990.1474993767498764No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT38300.14488910309105582No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG38090.14409467197750173No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34440.13028670262287106No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC33860.12809255954734072No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA33760.12771425901707686No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA33060.1250661553052299No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC32900.12446087445680773No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC30020.11356581918520875No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC27700.10478924688308736No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG27340.10342736497413749No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA27300.10327604476203195No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA27090.10248161364847785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87250.028.8790841
GTATTGG12150.023.1440331
ATTGGAC13000.021.9153843
GCGCTTA555.145526E-420.18181818
TTGGACC21400.018.5000024
TATTGGA16050.018.2118382
TGGACCC22000.017.7431835
GTATCAA143250.017.6024442
GGACCCT21500.017.2093016
CCGTTTA1107.8170524E-716.81818227
ACGTTTA1708.54925E-1116.3235326
TCACGTT1708.54925E-1116.3235324
CGTCTGT7200.016.187534
TTACGCT803.384647E-416.18754
TGCGACG1201.9377203E-615.41666722
TATACCG2300.015.2826095
ATCACGT2201.8189894E-1215.13636423
GACCCTC24200.014.9834717
CGGTTTA1252.9620842E-614.80000115
TCGGGTA1001.09421766E-414.825
Produced by FastQC (version 0.11.5)