##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088145_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2643401
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39239184671565	31.0	31.0	34.0	30.0	34.0
2	31.566065837154483	31.0	31.0	34.0	30.0	34.0
3	31.64727069407933	31.0	31.0	34.0	30.0	34.0
4	35.38141356532739	37.0	35.0	37.0	33.0	37.0
5	35.283383035718	37.0	35.0	37.0	33.0	37.0
6	35.31479559854899	37.0	35.0	37.0	32.0	37.0
7	35.1709460653151	37.0	35.0	37.0	32.0	37.0
8	35.24658460823765	37.0	35.0	37.0	32.0	37.0
9	36.67792627754926	39.0	35.0	39.0	32.0	39.0
10	36.54227376020513	38.0	35.0	39.0	32.0	39.0
11	36.62755594024516	38.0	35.0	39.0	32.0	39.0
12	36.50786543547498	38.0	35.0	39.0	32.0	39.0
13	36.532475776471294	38.0	35.0	39.0	32.0	39.0
14	37.45705210824994	39.0	36.0	41.0	32.0	41.0
15	37.47482315395961	39.0	36.0	41.0	32.0	41.0
16	37.33272515218085	39.0	36.0	41.0	32.0	41.0
17	37.40118809064535	39.0	36.0	41.0	32.0	41.0
18	37.386317853401735	39.0	36.0	40.0	32.0	41.0
19	37.44807995457367	39.0	36.0	41.0	32.0	41.0
20	37.372852246026994	39.0	36.0	41.0	32.0	41.0
21	37.29229012170306	39.0	36.0	41.0	31.0	41.0
22	37.19522085374107	39.0	36.0	40.0	31.0	41.0
23	36.96864531715014	39.0	36.0	40.0	31.0	41.0
24	36.82248323277475	39.0	35.0	40.0	30.0	41.0
25	36.71438347795132	39.0	35.0	40.0	30.0	41.0
26	36.81812596726717	39.0	36.0	40.0	31.0	41.0
27	36.70292475488963	39.0	35.0	40.0	30.0	41.0
28	36.60225557908164	39.0	35.0	40.0	30.0	41.0
29	36.51778561028009	39.0	35.0	40.0	30.0	41.0
30	36.30257800462359	38.0	35.0	40.0	30.0	41.0
31	36.09041496163465	38.0	35.0	40.0	30.0	41.0
32	35.9405784442088	38.0	35.0	40.0	29.0	41.0
33	35.71200358931543	38.0	34.0	40.0	28.0	41.0
34	35.56501113527611	38.0	34.0	40.0	27.0	41.0
35	35.333300925587906	38.0	34.0	40.0	27.0	41.0
36	35.237997564501185	38.0	34.0	40.0	26.0	41.0
37	35.05987967773334	38.0	34.0	40.0	25.0	41.0
38	34.91361280411107	38.0	33.0	40.0	25.0	41.0
39	34.782798750549006	38.0	33.0	40.0	24.0	41.0
40	34.545702297910914	38.0	33.0	40.0	23.0	41.0
41	34.3566609833317	38.0	33.0	40.0	22.0	41.0
42	34.108797341001235	38.0	33.0	40.0	21.0	41.0
43	33.776622994392454	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	9.0
14	13.0
15	9.0
16	40.0
17	125.0
18	231.0
19	587.0
20	1109.0
21	2198.0
22	4166.0
23	7106.0
24	11667.0
25	18054.0
26	26175.0
27	36336.0
28	49371.0
29	64002.0
30	80358.0
31	99084.0
32	120795.0
33	147556.0
34	179266.0
35	218401.0
36	280291.0
37	375145.0
38	485279.0
39	436025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.34885399528865	24.214827791924115	14.808460767019458	21.62785744576778
2	17.940940477816266	23.506762689429262	36.08200193614211	22.470294896612355
3	20.186267615091317	26.20071642554421	32.65815515693609	20.95486080242839
4	13.379090043470516	16.16394939700787	34.982774085354436	35.47418647416718
5	10.661984314903414	41.85732698141523	34.159024680704896	13.321664022976462
6	31.244937866029403	36.794984945530395	15.0984281234667	16.861649064973495
7	24.284548579651744	36.021927811936216	20.862290662672823	18.83123294573922
8	28.26094111336116	34.2780380275259	19.704199249376085	17.75682160973685
9	25.25496509988458	13.824538917856202	23.723264082899263	37.197231899359956
10	16.555074315247666	28.6362152393829	32.772742387553	22.035968057816426
11	31.216376175994487	21.929552118653206	24.871027891719795	21.983043813632513
12	19.610115907499466	28.200072558041704	31.731091877471485	20.45871965698734
13	31.262150540156412	21.037557298344066	29.082458544882144	18.61783361661738
14	20.453801750093913	22.809933112683247	28.71085393400396	28.025411203218887
15	23.03676211062945	29.270700888741434	26.90026976610813	20.792267234520985
16	21.45834097815655	28.79805220622978	27.055183833251178	22.688422982362493
17	19.063509471321225	27.3022519095665	28.216604291214235	25.417634327898035
18	20.23351735132127	26.591160402829537	32.66144637154938	20.513875874299814
19	19.49666357847334	24.62063077073815	34.05215478090536	21.830550869883155
20	24.60996269578471	23.696556065462637	32.5301382574948	19.16334298125786
21	23.66557325203403	24.67620311863391	31.200449723670374	20.457773905661682
22	21.764423937193033	24.093204171444288	32.541979064092054	21.600392827270625
23	20.663720714337323	26.503167699490167	33.60364167222453	19.229469913947977
24	19.362556040494802	25.285077822093584	31.842690533899322	23.509675603512292
25	20.51391370435284	26.560972020514484	32.834405373986016	20.090708901146666
26	22.266580061065273	25.253830198293787	31.372765615205562	21.106824125435377
27	20.70836017690846	27.011527952058728	30.997945449820136	21.282166421212672
28	20.57553886073282	26.257348014924712	31.141775311426457	22.025337812916014
29	19.391723011378144	26.08979871007085	32.497831392210266	22.020646886340742
30	18.719899099682568	26.313941774252186	34.512357375971334	20.453801750093913
31	20.22069296334533	25.67355463662153	33.27092635585747	20.834826044175667
32	21.561995323448848	25.88990470987943	31.077880351864888	21.470219614806833
33	22.668827014894827	25.884268031978504	30.487996335024466	20.95890861810221
34	20.62369651823541	28.911920665839197	30.66057703693083	19.80380577899456
35	19.20162699492056	29.749705020161528	30.93257511819054	20.11609286672737
36	20.160883649510613	25.603266398098512	33.44051848357476	20.79533146881612
37	20.249708614016566	26.143025594678974	32.425689481088945	21.181576310215515
38	20.612839293016837	25.404128998967618	33.319651464155456	20.66338024386009
39	21.83804122038238	24.919374699487516	33.09581104039834	20.14677303973177
40	19.87685561138851	25.858846236344768	31.910444158869577	22.353853993397145
41	20.883891622950888	25.443888384698347	31.82782332305995	21.84439666929081
42	18.36804177648416	27.355478794174626	32.23612308537373	22.04035634396749
43	19.215737604699402	27.028929776450866	31.640261920155133	22.1150706986946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	232.0
1	322.0
2	412.0
3	764.0
4	1116.0
5	1116.0
6	2318.0
7	3520.0
8	4092.0
9	4664.0
10	7737.5
11	10811.0
12	10811.0
13	22504.5
14	34198.0
15	54419.0
16	74640.0
17	69259.5
18	63879.0
19	63879.0
20	70637.0
21	77395.0
22	61596.5
23	45798.0
24	50167.0
25	54536.0
26	54536.0
27	59547.0
28	64558.0
29	68009.5
30	71461.0
31	76832.5
32	82204.0
33	82204.0
34	89119.0
35	96034.0
36	104448.0
37	112862.0
38	116362.0
39	119862.0
40	119862.0
41	126421.5
42	132981.0
43	153877.5
44	174774.0
45	226491.0
46	278208.0
47	278208.0
48	280449.5
49	282691.0
50	254815.5
51	226940.0
52	211583.5
53	196227.0
54	196227.0
55	167400.0
56	138573.0
57	113132.5
58	87692.0
59	75356.5
60	63021.0
61	63021.0
62	55124.5
63	47228.0
64	40630.5
65	34033.0
66	28758.0
67	23483.0
68	23483.0
69	19532.5
70	15582.0
71	12815.0
72	10048.0
73	8074.0
74	6100.0
75	6100.0
76	4634.5
77	3169.0
78	2493.0
79	1817.0
80	1469.0
81	1121.0
82	1121.0
83	842.5
84	564.0
85	459.0
86	354.0
87	293.5
88	233.0
89	233.0
90	234.5
91	236.0
92	156.5
93	77.0
94	60.5
95	44.0
96	44.0
97	28.5
98	13.0
99	11.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2643401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.70399992014715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.31235176532022	30.24809960321541
2	13.36207211111018	10.877795642872584
3	5.132793180356474	6.267756396100854
4	2.359768933293406	3.8420813788936212
5	1.3101097924525844	2.6663354443686997
6	0.7356725279017704	1.7966888710180868
7	0.4793634558706367	1.36584070486359
8	0.3447461273233035	1.122603707123103
9	0.24736114342801713	0.9061729166107365
>10	1.4334153594715047	11.166854005823703
>50	0.15983030437855722	4.577196532502574
>100	0.1057138376052841	8.05397151457287
>500	0.008307389291400936	2.309485342252713
>1k	0.007000608953427677	5.966265451748217
>5k	7.467316216989521E-4	2.230762385829379
>10k+	7.467316216989521E-4	6.6020901022038965
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38283	1.4482479200091094	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37730	1.4273279006855184	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28806	1.0897325074780557	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	20574	0.7783155109648516	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14950	0.5655592927444606	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11619	0.4395473861135711	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11320	0.4282362002586819	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10486	0.3966859360346765	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9771	0.369637448120811	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9029	0.3415675487752331	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8897	0.33657398177575026	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7403	0.2800558825543306	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6965	0.2634863193287738	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5834	0.22070052935593198	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5568	0.2106377352509135	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5247	0.1984942882294438	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4790	0.18120595399638573	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4664	0.17643936731506116	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4211	0.1593023532941086	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4107	0.15536802777936454	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3969	0.15014748046172335	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3899	0.1474993767498764	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3830	0.14488910309105582	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3809	0.14409467197750173	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3444	0.13028670262287106	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3386	0.12809255954734072	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3376	0.12771425901707686	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3306	0.1250661553052299	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3290	0.12446087445680773	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3002	0.11356581918520875	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2770	0.10478924688308736	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2734	0.10342736497413749	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2730	0.10327604476203195	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2709	0.10248161364847785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.566010605277065E-5	0.0
5	0.0	0.0	0.0	7.566010605277065E-5	0.0
6	0.0	0.0	0.0	7.566010605277065E-5	0.0
7	0.0	0.0	0.0	1.1349015907915598E-4	0.0
8	0.0	0.0	0.0	1.1349015907915598E-4	0.0
9	0.0	0.0	0.0	2.2698031815831195E-4	0.0
10	0.0	0.0	0.0	3.4047047723746797E-4	0.0
11	0.0	0.0	0.0	6.431109014485506E-4	0.0
12	0.0	0.0	0.0	9.835813786860186E-4	0.0
13	0.0	0.0	0.0	0.0012105616968443305	0.0
14	0.0	0.0	0.0	0.0014375420150026423	0.0
15	0.0	0.0	0.0	0.0022698031815831196	0.0
16	3.783005302638533E-5	0.0	0.0	0.0035181949314538356	0.0
17	3.783005302638533E-5	0.0	0.0	0.005296207423693946	0.0
18	3.783005302638533E-5	0.0	0.0	0.006279788802379964	0.0
19	3.783005302638533E-5	0.0	0.0	0.0069607297568549005	0.0
20	3.783005302638533E-5	0.0	0.0	0.009117042779358864	0.0
21	3.783005302638533E-5	0.0	0.0	0.012824387975944625	0.0
22	3.783005302638533E-5	0.0	0.0	0.022357561338593727	0.0
23	3.783005302638533E-5	0.0	0.0	0.036808641594672925	0.0
24	3.783005302638533E-5	0.0	0.0	0.05511838725944342	0.0
25	3.783005302638533E-5	0.0	0.0	0.06151166622090254	0.0
26	3.783005302638533E-5	0.0	0.0	0.06998559809881286	0.0
27	3.783005302638533E-5	0.0	0.0	0.11352798913218237	0.0
28	3.783005302638533E-5	0.0	0.0	0.19058780714692927	0.0
29	3.783005302638533E-5	0.0	0.0	0.28633567135671056	0.0
30	3.783005302638533E-5	0.0	0.0	0.47541027638258443	0.0
31	3.783005302638533E-5	0.0	0.0	0.7145718716153925	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8725	0.0	28.879084	1
GTATTGG	1215	0.0	23.144033	1
ATTGGAC	1300	0.0	21.915384	3
GCGCTTA	55	5.145526E-4	20.181818	18
TTGGACC	2140	0.0	18.500002	4
TATTGGA	1605	0.0	18.211838	2
TGGACCC	2200	0.0	17.743183	5
GTATCAA	14325	0.0	17.602444	2
GGACCCT	2150	0.0	17.209301	6
CCGTTTA	110	7.8170524E-7	16.818182	27
ACGTTTA	170	8.54925E-11	16.32353	26
TCACGTT	170	8.54925E-11	16.32353	24
CGTCTGT	720	0.0	16.1875	34
TTACGCT	80	3.384647E-4	16.1875	4
TGCGACG	120	1.9377203E-6	15.416667	22
TATACCG	230	0.0	15.282609	5
ATCACGT	220	1.8189894E-12	15.136364	23
GACCCTC	2420	0.0	14.983471	7
CGGTTTA	125	2.9620842E-6	14.800001	15
TCGGGTA	100	1.09421766E-4	14.8	25
>>END_MODULE