FastQCFastQC Report
Wed 25 May 2016
SRR2088144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3293634
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT423671.2863299322268351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT416381.2641963253962036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT320600.9733929149383326No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG226010.6862025349507566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166590.5057939042407261No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA126700.3846814794843628No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT123000.3734476872657982No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT111160.3374995521663913No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA109640.33288458887660255No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA95090.2887084600171118No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG92820.2818163766830194No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT79280.24070676948319092No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT78110.2371544622140772No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA64900.19704678783374233No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA63420.19255327094631644No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA58420.1773724706509588No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT51270.15566392622859734No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49900.15150438694766935No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC46420.14093854994210042No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA45430.1379327514836196No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG43190.1311317529512994No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42690.12961367292176362No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG41790.12688112886859923No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT41350.12554521844260777No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39140.11883530471205969No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC38750.11765120228902179No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC37950.11522227424176457No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA36310.11024297174488724No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA35440.10760151249349502No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC34950.10611379406454999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98150.028.6123281
GTATTGG15250.019.2885251
ATTGGAC15850.018.4416433
ACGTTTA2550.017.41176426
GTATCAA163200.017.207722
TTGGACC25800.016.133724
CGAATTA1851.8189894E-1116.015
CGTCTGT8800.015.76704534
TATTGGA18650.015.6729232
TAGGGCG750.004106567714.84
GGACCCT27100.014.7453886
TGGACCC28100.014.4181495
TGCGACG1551.2122291E-714.3225822
CGCACTA1458.9237255E-714.03448329
CGCGTAA800.00630191213.87510
TTTTTAC19550.013.7212291
ATTCCGT2355.638867E-1113.38297929
CGCGCTA2651.8189894E-1213.26415224
CCGTCGT1703.737332E-713.0588239
CGTTTAG1857.3965566E-813.026
Produced by FastQC (version 0.11.5)