##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088144_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3293634
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.426880460913384	31.0	31.0	34.0	30.0	34.0
2	31.609517025874762	31.0	31.0	34.0	30.0	34.0
3	31.697626998021033	31.0	31.0	34.0	30.0	34.0
4	35.43002561911858	37.0	35.0	37.0	33.0	37.0
5	35.32368623836164	37.0	35.0	37.0	33.0	37.0
6	35.345164641851525	37.0	35.0	37.0	33.0	37.0
7	35.20161286894658	37.0	35.0	37.0	32.0	37.0
8	35.26315492249594	37.0	35.0	37.0	32.0	37.0
9	36.72016259244349	39.0	35.0	39.0	32.0	39.0
10	36.576367319501806	38.0	35.0	39.0	32.0	39.0
11	36.6538650013936	39.0	35.0	39.0	32.0	39.0
12	36.53080427272733	38.0	35.0	39.0	32.0	39.0
13	36.55305962957633	38.0	35.0	39.0	32.0	39.0
14	37.496771954625196	39.0	36.0	41.0	32.0	41.0
15	37.50906202692831	39.0	36.0	41.0	32.0	41.0
16	37.374543437431115	39.0	36.0	41.0	32.0	41.0
17	37.42231802319262	39.0	36.0	41.0	32.0	41.0
18	37.399040694867736	39.0	36.0	41.0	32.0	41.0
19	37.44763625830921	39.0	36.0	41.0	32.0	41.0
20	37.35795446610036	39.0	36.0	41.0	31.0	41.0
21	37.278253442853696	39.0	36.0	41.0	31.0	41.0
22	37.174240671550024	39.0	36.0	40.0	31.0	41.0
23	36.94233239030202	39.0	36.0	40.0	31.0	41.0
24	36.79101078018991	39.0	35.0	40.0	30.0	41.0
25	36.68604344016366	39.0	35.0	40.0	30.0	41.0
26	36.80762646972918	39.0	36.0	40.0	30.0	41.0
27	36.70131137825271	39.0	35.0	40.0	30.0	41.0
28	36.60024003881427	39.0	35.0	40.0	30.0	41.0
29	36.51385855258963	39.0	35.0	40.0	30.0	41.0
30	36.31014466088217	38.0	35.0	40.0	30.0	41.0
31	36.118933372681965	38.0	35.0	40.0	30.0	41.0
32	35.963661718333	38.0	35.0	40.0	29.0	41.0
33	35.74739209031726	38.0	34.0	40.0	28.0	41.0
34	35.59933799566072	38.0	34.0	40.0	27.0	41.0
35	35.37951484591184	38.0	34.0	40.0	27.0	41.0
36	35.28007787143319	38.0	34.0	40.0	26.0	41.0
37	35.11192743334566	38.0	34.0	40.0	25.0	41.0
38	34.97765841620532	38.0	33.0	40.0	25.0	41.0
39	34.85619227880208	38.0	33.0	40.0	24.0	41.0
40	34.63540939885853	38.0	33.0	40.0	23.0	41.0
41	34.4698910079262	38.0	33.0	40.0	23.0	41.0
42	34.23444043873727	38.0	33.0	40.0	21.0	41.0
43	33.906958393069786	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	7.0
14	18.0
15	26.0
16	68.0
17	113.0
18	325.0
19	711.0
20	1390.0
21	2879.0
22	5200.0
23	9115.0
24	14665.0
25	22338.0
26	32316.0
27	44711.0
28	60791.0
29	78354.0
30	99272.0
31	122344.0
32	148835.0
33	182588.0
34	221737.0
35	269455.0
36	345357.0
37	463609.0
38	605466.0
39	561939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.14878398753474	23.46478084693078	14.13994390390675	22.246491261627735
2	18.287642160604367	23.165931612316363	35.57893803622382	22.967488190855452
3	20.17327972689133	25.974288582155758	32.08847734751342	21.763954343439494
4	13.58818253637168	15.925145295439627	34.958863067359644	35.52780910082905
5	11.185790528030742	41.26836800931737	34.089215741639784	13.456625721012111
6	31.905457619152582	36.54379934139616	15.040560062229137	16.510182977222122
7	25.164605417602566	35.169177874651524	20.80962851367213	18.85658819407378
8	28.423649986610535	34.06015968987447	19.503381371457788	18.01280895205721
9	25.521445309345243	13.641801123014883	23.00237366993418	37.834379897705695
10	16.596075945293254	28.34097534820202	32.779446653756914	22.283502052747817
11	32.15700348004666	21.694001215678487	24.274251480279837	21.874743823995015
12	19.977295595078264	27.715829992039186	31.35469818443701	20.95217622844554
13	31.35806832210258	20.77134253532724	28.302871539460668	19.567717603109514
14	20.867497724398035	22.333234354515408	28.028584839724147	28.770683081362407
15	23.43733395999677	28.953824256125603	26.145679817490347	21.46316196638728
16	22.142867118811623	28.34777634673434	26.411040206653198	23.09831632780084
17	19.774449741531694	26.966961113469196	27.639379481751767	25.619209663247343
18	20.85586923137179	26.34970977345995	31.675650664281456	21.118770330886797
19	20.25477026287681	24.486327260405982	32.89485109760222	22.364051379114983
20	24.76705061946774	23.80407173353202	31.512912485115223	19.91596516188502
21	24.031480121956477	24.616821419744877	30.29413711420273	21.057561344095912
22	22.371429248058526	24.07520082680711	31.433941961978775	22.11942796315559
23	21.28581985733691	26.193408253618948	32.41817396832799	20.102597920716146
24	20.194381039301877	25.16068877112636	30.802845732100163	23.842084457471596
25	21.233688989122655	26.246996478661565	31.728419126108122	20.790895406107662
26	22.77599757592981	25.11778782949168	30.422323791896734	21.68389080268178
27	21.282176465266026	26.71927117585014	30.076748053973212	21.921804304910626
28	21.24741243258966	26.01041888685871	30.25120581096746	22.490962869584173
29	20.14686513437741	25.94665952561821	31.41706091205034	22.489414427954046
30	19.559762863754745	26.101351880627906	33.13574003668896	21.203145218928395
31	20.973095371252544	25.500617251340007	32.151386583937374	21.37490079347007
32	21.987446085387752	25.62467475135367	30.20156459400164	22.186314569256936
33	22.988468056863635	25.70151996244877	29.7121659540799	21.59784602660769
34	21.210826703877846	28.336997978524632	29.874084370030186	20.578090947567336
35	20.00434778120459	29.129526838744074	30.09293078708806	20.77319459296327
36	20.910003965225037	25.409653895970223	32.2592309892356	21.42111114956914
37	20.903901283506304	25.918605406672384	31.438374755665023	21.73911855415629
38	21.14570107061076	25.29509957694146	32.2794821768296	21.279717175618178
39	22.21415615699862	24.830627811104694	32.113039882391305	20.842176149505377
40	20.51029349344827	25.564740951787602	31.176566673771283	22.74839888099285
41	21.469811156916645	25.191779050131252	30.991239463765556	22.347170329186547
42	19.152279822226756	26.894214718453842	31.36280473179473	22.590700727524673
43	19.853997135079368	26.544600887651754	30.884670245692142	22.716731731576733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	395.5
2	535.0
3	982.0
4	1429.0
5	1429.0
6	2734.5
7	4040.0
8	4658.5
9	5277.0
10	8794.5
11	12312.0
12	12312.0
13	24947.0
14	37582.0
15	60008.0
16	82434.0
17	76413.0
18	70392.0
19	70392.0
20	78047.5
21	85703.0
22	68157.5
23	50612.0
24	56193.5
25	61775.0
26	61775.0
27	67038.0
28	72301.0
29	77385.5
30	82470.0
31	88937.5
32	95405.0
33	95405.0
34	105312.0
35	115219.0
36	126524.5
37	137830.0
38	144359.5
39	150889.0
40	150889.0
41	160118.0
42	169347.0
43	192833.0
44	216319.0
45	272966.5
46	329614.0
47	329614.0
48	336019.5
49	342425.0
50	311717.5
51	281010.0
52	264173.0
53	247336.0
54	247336.0
55	215985.5
56	184635.0
57	155431.5
58	126228.0
59	111207.0
60	96186.0
61	96186.0
62	85171.5
63	74157.0
64	64191.5
65	54226.0
66	46114.0
67	38002.0
68	38002.0
69	31998.5
70	25995.0
71	21593.0
72	17191.0
73	13992.5
74	10794.0
75	10794.0
76	8258.5
77	5723.0
78	4532.5
79	3342.0
80	2640.0
81	1938.0
82	1938.0
83	1529.0
84	1120.0
85	849.0
86	578.0
87	486.5
88	395.0
89	395.0
90	385.0
91	375.0
92	243.5
93	112.0
94	108.0
95	104.0
96	104.0
97	56.5
98	9.0
99	10.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3293634.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.45153140935224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.76890102383341	29.436134853896416
2	13.806670356572399	11.170019191749219
3	5.480656209423362	6.651028103980517
4	2.656093250056482	4.29772158123313
5	1.418420825248249	2.868864728210444
6	0.8601845048923423	2.087746831049442
7	0.5596711173241705	1.5847687646942161
8	0.37055385698657967	1.1991576787799396
9	0.28292179060122774	1.030015772900617
>10	1.5120041276711123	11.554465828961257
>50	0.15935554230242258	4.481451346254532
>100	0.10892685924276195	8.185905660668102
>500	0.008196242342245908	2.221936230125501
>1k	0.006165980477531709	5.300763347517095
>5k	6.015590709787033E-4	1.77428214649945
>10k+	6.767539548510412E-4	6.155737933480075
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	42367	1.2863299322268351	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	41638	1.2641963253962036	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32060	0.9733929149383326	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	22601	0.6862025349507566	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16659	0.5057939042407261	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	12670	0.3846814794843628	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	12300	0.3734476872657982	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11116	0.3374995521663913	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10964	0.33288458887660255	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9509	0.2887084600171118	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	9282	0.2818163766830194	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7928	0.24070676948319092	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7811	0.2371544622140772	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6490	0.19704678783374233	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6342	0.19255327094631644	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5842	0.1773724706509588	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5127	0.15566392622859734	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4990	0.15150438694766935	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4642	0.14093854994210042	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4543	0.1379327514836196	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4319	0.1311317529512994	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4269	0.12961367292176362	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4179	0.12688112886859923	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4135	0.12554521844260777	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3914	0.11883530471205969	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3875	0.11765120228902179	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3795	0.11522227424176457	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3631	0.11024297174488724	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3544	0.10760151249349502	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3495	0.10611379406454999	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	6.07232011814306E-5	0.0	0.0	3.03616005907153E-5	0.0
7	6.07232011814306E-5	0.0	0.0	3.03616005907153E-5	0.0
8	6.07232011814306E-5	0.0	0.0	3.03616005907153E-5	0.0
9	6.07232011814306E-5	0.0	0.0	3.03616005907153E-5	0.0
10	6.07232011814306E-5	0.0	0.0	1.821696035442918E-4	0.0
11	6.07232011814306E-5	0.0	0.0	3.9470080767929893E-4	0.0
12	6.07232011814306E-5	0.0	0.0	6.375936124050213E-4	0.0
13	6.07232011814306E-5	0.0	0.0	6.983168135864519E-4	0.0
14	6.07232011814306E-5	0.0	0.0	8.501248165400285E-4	0.0
15	6.07232011814306E-5	0.0	0.0	0.0012751872248100427	0.0
16	6.07232011814306E-5	0.0	0.0	0.002307481644894363	0.0
17	6.07232011814306E-5	0.0	0.0	0.003825561674430128	0.0
18	6.07232011814306E-5	0.0	0.0	0.004645324890379441	0.0
19	6.07232011814306E-5	0.0	0.0	0.005465088106328754	0.0
20	6.07232011814306E-5	0.0	0.0	0.00704389133704595	0.0
21	6.07232011814306E-5	0.0	0.0	0.010292582600252486	0.0
22	6.07232011814306E-5	0.0	0.0	0.018368768357382756	0.0
23	6.07232011814306E-5	0.0	0.0	0.02869171255822596	0.0
24	6.07232011814306E-5	0.0	0.0	0.04451010646598863	0.0
25	6.07232011814306E-5	0.0	0.0	0.04976266336818238	0.0
26	6.07232011814306E-5	0.0	0.0	0.05659402350109332	0.0
27	6.07232011814306E-5	0.0	0.0	0.09700531388733538	0.0
28	6.07232011814306E-5	0.0	0.0	0.16793001286724632	0.0
29	6.07232011814306E-5	0.0	0.0	0.25807360502108007	0.0
30	6.07232011814306E-5	0.0	0.0	0.42579108668419136	0.0
31	6.07232011814306E-5	0.0	0.0	0.628667301831351	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9815	0.0	28.612328	1
GTATTGG	1525	0.0	19.288525	1
ATTGGAC	1585	0.0	18.441643	3
ACGTTTA	255	0.0	17.411764	26
GTATCAA	16320	0.0	17.20772	2
TTGGACC	2580	0.0	16.13372	4
CGAATTA	185	1.8189894E-11	16.0	15
CGTCTGT	880	0.0	15.767045	34
TATTGGA	1865	0.0	15.672923	2
TAGGGCG	75	0.0041065677	14.8	4
GGACCCT	2710	0.0	14.745388	6
TGGACCC	2810	0.0	14.418149	5
TGCGACG	155	1.2122291E-7	14.32258	22
CGCACTA	145	8.9237255E-7	14.034483	29
CGCGTAA	80	0.006301912	13.875	10
TTTTTAC	1955	0.0	13.721229	1
ATTCCGT	235	5.638867E-11	13.382979	29
CGCGCTA	265	1.8189894E-12	13.264152	24
CCGTCGT	170	3.737332E-7	13.058823	9
CGTTTAG	185	7.3965566E-8	13.0	26
>>END_MODULE