FastQCFastQC Report
Wed 25 May 2016
SRR2088143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3113624
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT439941.4129515959537824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT439421.4112815163295247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT335981.0790641387656312No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG236680.7601431643640979No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175280.5629453010382757No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT130320.41854764737168004No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA122770.39429937590409114No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT114220.3668394128513912No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA102540.32932685513729343No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA99290.3188888574856823No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG93480.3002289293761867No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT83030.2666667523117756No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT78700.2527601277482445No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA66120.21235704760754673No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA64940.2085672515371156No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT54220.17413791774472448No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53540.17195396746684893No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA52040.16713643008918225No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC44730.14365896460202002No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44430.14269545712648668No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA44420.1426633402106356No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG43500.13970858395233335No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41930.1346662281637089No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT41090.1319684072322156No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC40330.12952752162753112No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG39430.12663699920093113No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37550.12059901902092224No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA37380.12005303145145335No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC32900.10566465315015557No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC32360.10393033969419557No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC32020.10283836455525779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104950.028.6093371
GTATTGG14550.022.3780081
ATTGGAC15150.021.2475263
ACGTTTA1550.020.29032326
TATTGGA17200.019.2529072
TTGGACC23050.019.1019524
GGACCCT23750.018.5389486
TAGACCG609.24165E-418.55
TGGACCC24000.018.55
GTATCAA172100.017.468042
CGCGATA852.7255364E-517.41176414
CGTCTGT8650.017.10982734
TAGGTCG1251.6602644E-716.2821
GACCCTC27050.016.2772667
GACCGTC1701.4897523E-915.23529422
CTAGTAC3900.015.1794873
TAGTACT4900.015.102044
GTATTAG4150.014.7108431
TTTTTAC20800.014.4086541
CGCGCTT908.279615E-414.38888817
Produced by FastQC (version 0.11.5)