##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088143_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3113624
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30254680719316	31.0	31.0	33.0	30.0	34.0
2	31.471175389192787	31.0	31.0	34.0	30.0	34.0
3	31.549120253441007	31.0	31.0	34.0	30.0	34.0
4	35.314379321331025	37.0	35.0	37.0	33.0	37.0
5	35.19363320683551	37.0	35.0	37.0	33.0	37.0
6	35.22924637014617	37.0	35.0	37.0	32.0	37.0
7	35.069018288656565	37.0	35.0	37.0	32.0	37.0
8	35.12328495669355	37.0	35.0	37.0	32.0	37.0
9	36.562947549222386	39.0	35.0	39.0	32.0	39.0
10	36.4199749231121	38.0	35.0	39.0	32.0	39.0
11	36.52258943276388	38.0	35.0	39.0	32.0	39.0
12	36.40087563559376	38.0	35.0	39.0	32.0	39.0
13	36.4327632366657	38.0	35.0	39.0	32.0	39.0
14	37.325308386626006	39.0	36.0	41.0	32.0	41.0
15	37.34903443704185	39.0	36.0	41.0	32.0	41.0
16	37.194936511280744	39.0	36.0	40.0	31.0	41.0
17	37.278785428169876	39.0	36.0	40.0	32.0	41.0
18	37.2658429534202	39.0	36.0	40.0	32.0	41.0
19	37.32802419303037	39.0	36.0	40.0	31.0	41.0
20	37.24116014008114	39.0	36.0	40.0	31.0	41.0
21	37.15259453293012	39.0	36.0	40.0	31.0	41.0
22	37.0896797429619	39.0	36.0	40.0	31.0	41.0
23	36.867440641516126	39.0	35.0	40.0	31.0	41.0
24	36.73144059783712	39.0	35.0	40.0	30.0	41.0
25	36.62209309794632	38.0	35.0	40.0	30.0	41.0
26	36.667460810939275	39.0	35.0	40.0	30.0	41.0
27	36.541710238615835	39.0	35.0	40.0	30.0	41.0
28	36.4146862305789	38.0	35.0	40.0	30.0	41.0
29	36.30641464736911	38.0	35.0	40.0	30.0	41.0
30	36.12358557102592	38.0	35.0	40.0	30.0	41.0
31	35.93635230201206	38.0	35.0	40.0	29.0	41.0
32	35.79732909304399	38.0	34.0	40.0	29.0	41.0
33	35.574929407018956	38.0	34.0	40.0	27.0	41.0
34	35.421787280673584	38.0	34.0	40.0	27.0	41.0
35	35.19423379316192	38.0	34.0	40.0	26.0	41.0
36	35.09008056207172	38.0	34.0	40.0	25.0	41.0
37	34.919241051584905	38.0	33.0	40.0	25.0	41.0
38	34.75413858577657	38.0	33.0	40.0	24.0	41.0
39	34.6344471907976	38.0	33.0	40.0	24.0	41.0
40	34.39741246855754	38.0	33.0	40.0	23.0	41.0
41	34.23623115700547	38.0	33.0	40.0	22.0	41.0
42	34.00650303312153	38.0	33.0	40.0	21.0	41.0
43	33.659322384462605	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	9.0
13	7.0
14	12.0
15	27.0
16	48.0
17	147.0
18	310.0
19	742.0
20	1463.0
21	2971.0
22	5338.0
23	9126.0
24	14846.0
25	22518.0
26	32887.0
27	45570.0
28	61738.0
29	80368.0
30	100188.0
31	123588.0
32	149144.0
33	180649.0
34	216480.0
35	261377.0
36	327504.0
37	430545.0
38	543280.0
39	502740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.11617973139981	23.569384100328104	14.451231105618403	21.86320506265368
2	18.200848914319774	23.26221791712808	35.577320832573236	22.95961233597891
3	20.380238590144476	25.95181691816353	32.22229145201862	21.445653039673385
4	13.498803966053705	15.913514284319493	34.61182210825713	35.975859641369674
5	10.984980845471387	41.519399901850704	33.943083686405295	13.552535566272613
6	31.795650341852454	36.234400813971114	15.096684763478185	16.87326408069825
7	24.75119025290144	35.60195450703104	20.68422519867524	18.96263004139228
8	28.491943792827907	33.9316500643623	19.686770143087283	17.889635999722508
9	25.44215358052225	13.645064400839665	23.423412717784807	37.489369300853284
10	16.507516642985795	28.421382928703014	32.8129536514364	22.258146776874792
11	31.644636603520524	21.71697674478357	24.575061086373946	22.063325565321954
12	19.737450636300334	27.955719765777754	31.62295768532103	20.68387191260088
13	31.32154043005835	20.882033283402237	28.78542816987536	19.010998116664055
14	20.652300984319236	22.57581519155813	28.573745577500688	28.198138246621944
15	23.23157837940612	28.855314578767377	26.986816648381435	20.926290393445065
16	21.70082193611046	28.528846129140835	26.911438246878877	22.858893687869827
17	19.208709850643494	27.06498922156304	28.086821016281995	25.639479911511476
18	20.328144952633973	26.42175163089699	32.43384557672988	20.81625783973916
19	19.77878510700072	24.564912140965	33.777842154351326	21.87846059768296
20	24.63984090564564	23.53720295064529	32.43773172354786	19.3852244201612
21	23.71602993810428	24.658243898428324	30.963790104392825	20.66193605907457
22	21.765055767812683	23.950515540733242	32.39803521555589	21.88639347589818
23	20.698645693892388	26.295885437676482	33.50504107111199	19.500427797319137
24	19.659599232277245	25.2543659735408	31.595979476006093	23.490055318175862
25	20.63974327022145	26.473684683828235	32.68233415466993	20.204237891280385
26	22.30111921028358	25.209434408265096	31.23822915034057	21.251217231110758
27	20.88049167144138	26.876013288695106	30.779246305912338	21.464248733951177
28	20.58536933168552	26.09512259669119	31.097075305174936	22.222432766448357
29	19.531645439526415	25.975776137388458	32.449358047085965	22.043220375999155
30	18.944837270010765	26.057192519071027	34.33805751754226	20.65991269337595
31	20.363088157079982	25.61783311022782	32.986898867686016	21.032179865006178
32	21.634050868056	25.77353591827401	30.915454146036904	21.676959067633085
33	22.649523513436435	25.747071579612697	30.539589879831347	21.063815027119524
34	20.680371168773107	28.66566419066657	30.591651400426002	20.062313240134326
35	19.32767090695601	29.352645020721834	30.857226177598836	20.462457894723318
36	20.189913746810788	25.555622644224226	33.23272174161042	21.021741867354567
37	20.35435235596848	25.878879402265653	32.326735662366424	21.44003257939944
38	20.727647268905944	25.148251683568727	33.260342289242374	20.863758758282952
39	21.83144143287693	24.79364881565661	32.9028167819878	20.472092969478652
40	20.05948695153943	25.566895681687964	31.866243322893194	22.507374043879416
41	20.93245684128848	25.275755839497638	31.68889371356336	22.10289360565052
42	18.53801229692474	27.020956929931167	32.08528068899778	22.35575008414632
43	19.362710462149572	26.616315907123017	31.56495453529392	22.45601909543349
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	394.0
1	473.0
2	552.0
3	1019.5
4	1487.0
5	1487.0
6	3018.0
7	4549.0
8	5279.5
9	6010.0
10	9842.5
11	13675.0
12	13675.0
13	27383.0
14	41091.0
15	64785.5
16	88480.0
17	82357.0
18	76234.0
19	76234.0
20	83362.0
21	90490.0
22	71503.5
23	52517.0
24	57456.5
25	62396.0
26	62396.0
27	67013.0
28	71630.0
29	75295.0
30	78960.0
31	85227.0
32	91494.0
33	91494.0
34	99201.0
35	106908.0
36	117126.0
37	127344.0
38	133568.0
39	139792.0
40	139792.0
41	146859.5
42	153927.0
43	178023.0
44	202119.0
45	261665.0
46	321211.0
47	321211.0
48	323219.0
49	325227.0
50	294913.5
51	264600.0
52	245941.5
53	227283.0
54	227283.0
55	197763.0
56	168243.0
57	140317.5
58	112392.0
59	99034.5
60	85677.0
61	85677.0
62	74601.0
63	63525.0
64	55177.5
65	46830.0
66	39670.0
67	32510.0
68	32510.0
69	27017.5
70	21525.0
71	17888.0
72	14251.0
73	11582.0
74	8913.0
75	8913.0
76	6910.5
77	4908.0
78	3817.0
79	2726.0
80	2163.0
81	1600.0
82	1600.0
83	1189.0
84	778.0
85	640.0
86	502.0
87	400.5
88	299.0
89	299.0
90	335.0
91	371.0
92	235.0
93	99.0
94	89.5
95	80.0
96	80.0
97	46.5
98	13.0
99	12.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3113624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.68608653096532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.79350359687203	30.761623763623085
2	13.589902674113407	11.33019717640977
3	5.363663383306106	6.707704077584056
4	2.4042812527871935	4.009003053938586
5	1.3169838684067734	2.74499517491451
6	0.8066919498887761	2.0176698256138
7	0.49992930421081255	1.4588067364288053
8	0.3436379117142395	1.1459935778432009
9	0.24998117254459687	0.9378663110825615
>10	1.3832380088666711	10.898711640956
>50	0.14125520188867033	4.136556653701538
>100	0.0913793078634221	7.1847620274132895
>500	0.007582746361503989	2.1022899261876478
>1k	0.0065768718441407276	5.717965401729417
>5k	6.963746658501948E-4	2.0815824768497126
>10k+	6.963746658501948E-4	6.764272175724208
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	43994	1.4129515959537824	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43942	1.4112815163295247	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	33598	1.0790641387656312	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	23668	0.7601431643640979	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17528	0.5629453010382757	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	13032	0.41854764737168004	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	12277	0.39429937590409114	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11422	0.3668394128513912	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10254	0.32932685513729343	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9929	0.3188888574856823	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	9348	0.3002289293761867	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8303	0.2666667523117756	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7870	0.2527601277482445	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6612	0.21235704760754673	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6494	0.2085672515371156	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5422	0.17413791774472448	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5354	0.17195396746684893	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5204	0.16713643008918225	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4473	0.14365896460202002	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4443	0.14269545712648668	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4442	0.1426633402106356	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4350	0.13970858395233335	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4193	0.1346662281637089	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4109	0.1319684072322156	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4033	0.12952752162753112	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3943	0.12663699920093113	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3755	0.12059901902092224	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3738	0.12005303145145335	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3290	0.10566465315015557	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3236	0.10393033969419557	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	3202	0.10283836455525779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.423383170222224E-5	0.0
3	0.0	0.0	0.0	6.423383170222224E-5	0.0
4	0.0	0.0	0.0	9.635074755333335E-5	0.0
5	0.0	0.0	0.0	9.635074755333335E-5	0.0
6	0.0	0.0	0.0	9.635074755333335E-5	0.0
7	0.0	0.0	0.0	1.2846766340444447E-4	0.0
8	0.0	0.0	0.0	1.2846766340444447E-4	0.0
9	3.211691585111112E-5	0.0	0.0	1.927014951066667E-4	0.0
10	3.211691585111112E-5	0.0	0.0	3.854029902133334E-4	0.0
11	3.211691585111112E-5	3.211691585111112E-5	0.0	6.423383170222224E-4	0.0
12	3.211691585111112E-5	3.211691585111112E-5	0.0	0.001124092054788889	0.0
13	3.211691585111112E-5	3.211691585111112E-5	0.0	0.0011883258864911113	0.0
14	6.423383170222224E-5	3.211691585111112E-5	0.0	0.0012846766340444448	0.0
15	6.423383170222224E-5	3.211691585111112E-5	0.0	0.0018948980352155558	0.0
16	9.635074755333335E-5	3.211691585111112E-5	0.0	0.0029547562583022227	0.0
17	9.635074755333335E-5	3.211691585111112E-5	0.0	0.005395641862986668	0.0
18	9.635074755333335E-5	3.211691585111112E-5	0.0	0.006070097095860001	0.0
19	9.635074755333335E-5	3.211691585111112E-5	0.0	0.00719418915064889	0.0
20	9.635074755333335E-5	3.211691585111112E-5	0.0	0.009602957839482224	0.0
21	9.635074755333335E-5	3.211691585111112E-5	0.0	0.013585455405020002	0.0
22	2.5693532680888894E-4	3.211691585111112E-5	0.0	0.023124179412800004	0.0
23	2.5693532680888894E-4	3.211691585111112E-5	0.0	0.03616364724835112	0.0
24	3.532860743622223E-4	3.211691585111112E-5	0.0	0.05360313255550445	0.0
25	3.532860743622223E-4	3.211691585111112E-5	0.0	0.06086155553785556	0.0
26	3.532860743622223E-4	3.211691585111112E-5	0.0	0.0696294735652089	0.0
27	3.532860743622223E-4	3.211691585111112E-5	0.0	0.11822236724794002	0.0
28	3.532860743622223E-4	3.211691585111112E-5	0.0	0.20374971415944892	0.0
29	3.532860743622223E-4	3.211691585111112E-5	0.0	0.30456471301608673	0.0
30	3.532860743622223E-4	3.211691585111112E-5	0.0	0.5018589270894623	0.0
31	5.138706536177779E-4	3.211691585111112E-5	0.0	0.7418365223289646	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10495	0.0	28.609337	1
GTATTGG	1455	0.0	22.378008	1
ATTGGAC	1515	0.0	21.247526	3
ACGTTTA	155	0.0	20.290323	26
TATTGGA	1720	0.0	19.252907	2
TTGGACC	2305	0.0	19.101952	4
GGACCCT	2375	0.0	18.538948	6
TAGACCG	60	9.24165E-4	18.5	5
TGGACCC	2400	0.0	18.5	5
GTATCAA	17210	0.0	17.46804	2
CGCGATA	85	2.7255364E-5	17.411764	14
CGTCTGT	865	0.0	17.109827	34
TAGGTCG	125	1.6602644E-7	16.28	21
GACCCTC	2705	0.0	16.277266	7
GACCGTC	170	1.4897523E-9	15.235294	22
CTAGTAC	390	0.0	15.179487	3
TAGTACT	490	0.0	15.10204	4
GTATTAG	415	0.0	14.710843	1
TTTTTAC	2080	0.0	14.408654	1
CGCGCTT	90	8.279615E-4	14.388888	17
>>END_MODULE