FastQCFastQC Report
Wed 25 May 2016
SRR2088142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921069
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT369321.2643316539253266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT367711.2588199730988894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT297821.0195582507636758No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG232280.7951883368725629No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158730.5433969550188646No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT116990.400504062040301No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT104220.3567871898952062No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA100090.34264853038391085No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA90460.3096811475524885No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG88700.30365595609004786No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA86700.2968091476100017No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT78460.26860029667221147No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT75620.25887782863054587No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA62370.21351772245023995No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA60140.2058835309949885No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT48490.1660008715987195No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA47590.16291980778269874No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42460.1453577440313803No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40770.13957219086574127No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC39580.1354983398201138No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG38190.1307398079264817No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA36830.1260839781600503No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36040.12337948881043206No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA35590.12183895690242169No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC34860.11933987180720483No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG34770.11903176542560276No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT34580.11838131861999837No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA31000.10612553144071571No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG30990.10609129739831548No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC29820.10208591443748848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88200.028.5891
GTATTGG12850.022.4591451
ATTGGAC13250.021.3622653
TTGGACC20200.018.9579224
ACGTTTA1306.9849193E-1018.526
TATTGGA16350.018.3302762
TGGACCC20550.018.2749395
GGACCCT20250.018.2716056
ACGGACC2800.017.8392878
TATACCG1603.45608E-1117.343755
GTATCAA148700.016.9448552
CGGTATA2100.015.85714226
GACCCTC23800.015.5462197
CGGACCA2750.015.4727289
TATTCCG855.3676654E-415.2352955
GACGGAC3050.015.1639347
TTTTTAC17150.014.9941691
CGTCTGT7900.014.98734234
TCTATAC2850.014.9298243
CTAACGG1001.09428074E-414.79999927
Produced by FastQC (version 0.11.5)