##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088142_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2921069
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60750464983881	31.0	31.0	34.0	30.0	34.0
2	31.77114474187361	31.0	31.0	34.0	30.0	34.0
3	31.859881776157977	31.0	31.0	34.0	30.0	34.0
4	35.565460453005386	37.0	35.0	37.0	33.0	37.0
5	35.46713480578514	37.0	35.0	37.0	33.0	37.0
6	35.49321258758352	37.0	35.0	37.0	33.0	37.0
7	35.349086584397696	37.0	35.0	37.0	33.0	37.0
8	35.413049469218286	37.0	35.0	37.0	33.0	37.0
9	36.90724662786124	39.0	37.0	39.0	33.0	39.0
10	36.78806286328738	39.0	37.0	39.0	32.0	39.0
11	36.85592466319693	39.0	37.0	39.0	33.0	39.0
12	36.7425391183844	39.0	35.0	39.0	32.0	39.0
13	36.76522533360218	39.0	37.0	39.0	32.0	39.0
14	37.76273172595375	40.0	37.0	41.0	32.0	41.0
15	37.7699735952831	40.0	37.0	41.0	32.0	41.0
16	37.6457502373275	39.0	36.0	41.0	32.0	41.0
17	37.68256929226937	39.0	37.0	41.0	32.0	41.0
18	37.66903589062771	39.0	37.0	41.0	32.0	41.0
19	37.70733796428636	39.0	37.0	41.0	32.0	41.0
20	37.62775442825897	39.0	37.0	41.0	32.0	41.0
21	37.555515121347696	39.0	36.0	41.0	32.0	41.0
22	37.453593187973304	39.0	36.0	41.0	32.0	41.0
23	37.236521629581496	39.0	36.0	41.0	31.0	41.0
24	37.088311505137334	39.0	36.0	41.0	31.0	41.0
25	36.983146238585945	39.0	36.0	40.0	31.0	41.0
26	37.09921299359926	39.0	36.0	41.0	31.0	41.0
27	36.996830954695014	39.0	36.0	41.0	31.0	41.0
28	36.90848863891952	39.0	35.0	41.0	31.0	41.0
29	36.821875484625664	39.0	35.0	41.0	30.0	41.0
30	36.62595987975635	39.0	35.0	40.0	30.0	41.0
31	36.44439244673782	39.0	35.0	40.0	30.0	41.0
32	36.297537648032275	38.0	35.0	40.0	30.0	41.0
33	36.071509094786876	38.0	35.0	40.0	30.0	41.0
34	35.93801618517057	38.0	35.0	40.0	29.0	41.0
35	35.71652124616023	38.0	35.0	40.0	27.0	41.0
36	35.62369666721327	38.0	35.0	40.0	27.0	41.0
37	35.453734232227994	38.0	34.0	40.0	26.0	41.0
38	35.31729308688018	38.0	34.0	40.0	25.0	41.0
39	35.191130028082185	38.0	34.0	40.0	25.0	41.0
40	34.979974112217135	38.0	34.0	40.0	24.0	41.0
41	34.812607644667075	38.0	33.0	40.0	23.0	41.0
42	34.58569585312774	38.0	33.0	40.0	23.0	41.0
43	34.265263504559464	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	7.0
13	10.0
14	11.0
15	16.0
16	31.0
17	101.0
18	215.0
19	542.0
20	1036.0
21	2066.0
22	3866.0
23	6837.0
24	10973.0
25	16866.0
26	25151.0
27	35230.0
28	47796.0
29	63404.0
30	79693.0
31	99774.0
32	122431.0
33	151548.0
34	186511.0
35	227475.0
36	292599.0
37	403859.0
38	533459.0
39	609560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.848744072803484	22.71952494104042	13.839145874335731	22.592585111820366
2	17.93757696240657	22.66920089871208	36.06573483885523	23.32748730002612
3	19.708709380024917	25.91181516081955	32.05083481424095	22.328640644914586
4	13.037692707703927	15.500318547764532	35.06685394970129	36.39513479483025
5	11.041984971940067	41.2369238795797	34.21695961307316	13.50413153540707
6	31.835160347119494	36.313281199451296	15.325519527268955	16.526038926160254
7	25.393580226964858	35.147440885511436	20.78246011990816	18.676518767615555
8	28.483544894009693	33.96215563548824	19.570848891279187	17.983450579222882
9	25.63653922587929	13.6862908750187	22.837632387321214	37.83953751178079
10	16.35794977797512	28.168660172012373	32.88398870413537	22.589401345877143
11	31.970692920982007	21.729955711419347	24.585143315683403	21.71420805191524
12	20.058410123143275	27.449984919904324	31.654541539415877	20.837063417536523
13	31.33712349828094	20.718373992534925	28.39713817099151	19.547364338192626
14	20.82295899206763	22.203172879517737	28.14099906575298	28.832869062661647
15	23.402699491179426	28.716747190840064	26.568971838734377	21.311581479246126
16	22.132000305367658	28.381219341275404	26.444770732906342	23.042009620450596
17	19.7214102097554	26.98067043263956	27.574562600198764	25.72335675740628
18	20.829566162250874	26.35898022265136	31.743789687953278	21.06766392714448
19	20.21787229264355	24.489356465047557	32.87546442757771	22.417306814731184
20	24.926764824795306	23.43679659741006	31.652179390490264	19.984259187304374
21	23.838635787103968	24.697978719434563	30.43567269379806	21.027712799663412
22	22.33261179383301	23.919701999507716	31.72927445397558	22.018411752683694
23	21.085979139828602	26.002980415731365	32.712784258091816	20.198256186348218
24	19.98549161283078	25.06869916458666	31.04082786130694	23.904981361275617
25	21.215863096695077	26.18490011704619	31.97541721883324	20.623819567425485
26	22.7310960473717	24.981299654338873	30.62625360784014	21.661350690449286
27	21.40302060649714	26.619843625741126	30.13927435469686	21.837861413064875
28	21.00392014019525	25.751120565792867	30.530980267840302	22.713979026171582
29	19.99689154895006	25.705726225570157	31.93207692115455	22.36530530432523
30	19.426038891926208	25.891514373676213	33.62710706251718	21.055339671880397
31	20.801460013440284	25.493201290349525	32.31204055775471	21.393298138455478
32	22.183454071095206	25.527058758283356	30.411058417312294	21.878428753309148
33	23.144472109354485	25.404877460956932	29.95430782360841	21.49634260608017
34	21.18337499045726	28.147948576360232	30.16601114181144	20.50266529137107
35	20.048482250847208	28.925437913311875	30.345260587819052	20.680819248021873
36	20.766438588064847	25.256712525448734	32.63507298184329	21.34177590464313
37	21.01870924651215	25.632773481215267	31.6573829649351	21.691134307337485
38	21.088101650457418	25.090540483638012	32.59940110966225	21.22195675624232
39	22.183145964713603	24.5990423368979	32.345179110798135	20.872632587590363
40	20.54710107840657	25.228914483019743	31.34510003016019	22.878884408413494
41	21.44393028716542	24.994650930874965	31.199571115916807	22.36184766604281
42	19.10892895717287	26.74055285924434	31.63567858205335	22.51483960152944
43	20.04598316575199	26.312524627114254	31.038020669830118	22.603471537303637
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	190.0
1	328.0
2	466.0
3	942.5
4	1419.0
5	1419.0
6	2651.0
7	3883.0
8	4576.5
9	5270.0
10	8599.5
11	11929.0
12	11929.0
13	23430.0
14	34931.0
15	53774.5
16	72618.0
17	68363.5
18	64109.0
19	64109.0
20	69051.0
21	73993.0
22	58986.5
23	43980.0
24	48780.5
25	53581.0
26	53581.0
27	58994.0
28	64407.0
29	68941.5
30	73476.0
31	79006.0
32	84536.0
33	84536.0
34	93010.5
35	101485.0
36	110545.5
37	119606.0
38	126406.0
39	133206.0
40	133206.0
41	141170.0
42	149134.0
43	171394.5
44	193655.0
45	247827.0
46	301999.0
47	301999.0
48	302885.0
49	303771.0
50	275216.0
51	246661.0
52	230752.5
53	214844.0
54	214844.0
55	188201.0
56	161558.0
57	136176.0
58	110794.0
59	98962.5
60	87131.0
61	87131.0
62	76332.0
63	65533.0
64	56919.5
65	48306.0
66	40804.0
67	33302.0
68	33302.0
69	28007.0
70	22712.0
71	19259.5
72	15807.0
73	12889.5
74	9972.0
75	9972.0
76	7724.0
77	5476.0
78	4265.0
79	3054.0
80	2424.0
81	1794.0
82	1794.0
83	1386.5
84	979.0
85	750.5
86	522.0
87	458.5
88	395.0
89	395.0
90	375.5
91	356.0
92	235.0
93	114.0
94	95.0
95	76.0
96	76.0
97	46.5
98	17.0
99	19.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2921069.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.872163639060034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.02745968535545	30.996899057291227
2	13.442191607212376	11.25707293289592
3	5.100286578025513	6.406801026035574
4	2.4282363141945327	4.067020332090458
5	1.282247363943994	2.684523572440814
6	0.8062450412108423	2.0255534579256653
7	0.5158701005898986	1.5120418087879013
8	0.3705255916477275	1.2411766564746545
9	0.2661091954105119	1.0028310998478802
>10	1.500915052756501	11.875813414282888
>50	0.1512239015368052	4.399499639152413
>100	0.09416347405006732	7.363417202769934
>500	0.007139944540788394	1.9828591000878533
>1k	0.006155124604122765	5.316524209762091
>5k	5.744782963847914E-4	1.864063496658617
>10k+	6.565466244397615E-4	6.003902993496302
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36932	1.2643316539253266	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36771	1.2588199730988894	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29782	1.0195582507636758	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	23228	0.7951883368725629	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15873	0.5433969550188646	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11699	0.400504062040301	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10422	0.3567871898952062	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10009	0.34264853038391085	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9046	0.3096811475524885	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8870	0.30365595609004786	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8670	0.2968091476100017	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7846	0.26860029667221147	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7562	0.25887782863054587	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6237	0.21351772245023995	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6014	0.2058835309949885	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4849	0.1660008715987195	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4759	0.16291980778269874	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4246	0.1453577440313803	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4077	0.13957219086574127	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3958	0.1354983398201138	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3819	0.1307398079264817	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3683	0.1260839781600503	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3604	0.12337948881043206	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3559	0.12183895690242169	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3486	0.11933987180720483	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3477	0.11903176542560276	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3458	0.11838131861999837	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3100	0.10612553144071571	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3099	0.10609129739831548	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2982	0.10208591443748848	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.423404240023087E-5	0.0
3	0.0	0.0	0.0	3.423404240023087E-5	0.0
4	0.0	0.0	0.0	3.423404240023087E-5	0.0
5	0.0	0.0	0.0	3.423404240023087E-5	0.0
6	3.423404240023087E-5	0.0	0.0	3.423404240023087E-5	0.0
7	3.423404240023087E-5	0.0	0.0	3.423404240023087E-5	0.0
8	3.423404240023087E-5	0.0	0.0	3.423404240023087E-5	0.0
9	3.423404240023087E-5	0.0	0.0	1.0270212720069263E-4	0.0
10	3.423404240023087E-5	0.0	0.0	3.4234042400230875E-4	0.0
11	3.423404240023087E-5	0.0	0.0	6.846808480046175E-4	0.0
12	3.423404240023087E-5	0.0	0.0	9.585531872064645E-4	0.0
13	3.423404240023087E-5	0.0	0.0	0.0012666595688085424	0.0
14	3.423404240023087E-5	0.0	3.423404240023087E-5	0.0016089999928108512	0.0
15	3.423404240023087E-5	0.0	3.423404240023087E-5	0.002464851052816623	0.0
16	3.423404240023087E-5	0.0	3.423404240023087E-5	0.00390268083362632	0.0
17	3.423404240023087E-5	0.0	3.423404240023087E-5	0.0066756382680450204	0.0
18	3.423404240023087E-5	0.0	3.423404240023087E-5	0.007599957412851254	0.0
19	3.423404240023087E-5	0.0	3.423404240023087E-5	0.009106255278461413	0.0
20	3.423404240023087E-5	0.0	3.423404240023087E-5	0.01174227654327919	0.0
21	3.423404240023087E-5	0.0	3.423404240023087E-5	0.01643234035211082	0.0
22	3.423404240023087E-5	0.0	3.423404240023087E-5	0.02711336158098285	0.0
23	3.423404240023087E-5	0.0	3.423404240023087E-5	0.044743893417101756	0.0
24	3.423404240023087E-5	0.0	3.423404240023087E-5	0.06867348905486313	0.0
25	3.423404240023087E-5	0.0	3.423404240023087E-5	0.07644461667971554	0.0
26	3.423404240023087E-5	0.0	3.423404240023087E-5	0.08318872303256103	0.0
27	6.846808480046175E-5	0.0	3.423404240023087E-5	0.1233110207256316	0.0
28	6.846808480046175E-5	0.0	3.423404240023087E-5	0.20040608421095155	0.0
29	6.846808480046175E-5	0.0	3.423404240023087E-5	0.29547401995639266	0.0
30	6.846808480046175E-5	0.0	3.423404240023087E-5	0.47674327446561515	0.0
31	6.846808480046175E-5	0.0	3.423404240023087E-5	0.694985294767087	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8820	0.0	28.589	1
GTATTGG	1285	0.0	22.459145	1
ATTGGAC	1325	0.0	21.362265	3
TTGGACC	2020	0.0	18.957922	4
ACGTTTA	130	6.9849193E-10	18.5	26
TATTGGA	1635	0.0	18.330276	2
TGGACCC	2055	0.0	18.274939	5
GGACCCT	2025	0.0	18.271605	6
ACGGACC	280	0.0	17.839287	8
TATACCG	160	3.45608E-11	17.34375	5
GTATCAA	14870	0.0	16.944855	2
CGGTATA	210	0.0	15.857142	26
GACCCTC	2380	0.0	15.546219	7
CGGACCA	275	0.0	15.472728	9
TATTCCG	85	5.3676654E-4	15.235295	5
GACGGAC	305	0.0	15.163934	7
TTTTTAC	1715	0.0	14.994169	1
CGTCTGT	790	0.0	14.987342	34
TCTATAC	285	0.0	14.929824	3
CTAACGG	100	1.09428074E-4	14.799999	27
>>END_MODULE