FastQCFastQC Report
Wed 25 May 2016
SRR2088141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5219372
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT515510.9876858748523769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT514960.9866321082306454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT415590.7962452187734463No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG330550.6333137396606335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194440.37253523987177No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA174580.33448468513070156No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT168150.32216519535300414No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA154110.29526540740916724No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT140120.26846141643094223No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG127670.24460797199356552No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA125080.23964568917486628No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT106600.20423913068468774No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT103660.1986062691067048No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA101800.19504262198594008No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA86550.16582454747429384No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA81680.15649392302368945No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG67240.12882775935495688No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT65480.12545570616541607No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG64660.12388463592938001No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC61250.11735128287464469No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60890.11666154472223861No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA56790.10880619354205832No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT52300.10020362603010477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125050.029.0851651
ATTGGAC21100.020.9549753
GTATTGG20050.020.8528671
GTATCAA205650.017.6678832
TTGGACC33300.017.611114
TATTGGA26700.016.8370782
TGGACCC33050.016.7927385
GGACCCT32100.016.7710296
ACGTTTA3500.015.85714326
TATACTG6450.014.9147285
GACCCTC35900.014.8927587
TCACGTT4200.013.65476124
TATACCG3400.013.6029415
CGTCTGT13050.013.32567134
ACGGACC5850.013.2820518
CGACGAG2950.013.16949224
CTGTGCG3800.013.1447379
TACGTTA2408.185452E-1113.10416719
ACCCTCG39850.013.0915948
ATAACGC6100.012.7377043
Produced by FastQC (version 0.11.5)