##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088141_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5219372
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.524209042773727	31.0	31.0	34.0	30.0	34.0
2	31.695594029320002	31.0	31.0	34.0	30.0	34.0
3	31.786057211480614	31.0	31.0	34.0	30.0	34.0
4	35.51837117568934	37.0	35.0	37.0	33.0	37.0
5	35.39615493971306	37.0	35.0	37.0	33.0	37.0
6	35.41107570029498	37.0	35.0	37.0	33.0	37.0
7	35.28207014177185	37.0	35.0	37.0	32.0	37.0
8	35.30298204458314	37.0	35.0	37.0	32.0	37.0
9	36.81808865127835	39.0	37.0	39.0	32.0	39.0
10	36.66846586141015	39.0	35.0	39.0	32.0	39.0
11	36.752824286140175	39.0	35.0	39.0	32.0	39.0
12	36.63562838594375	39.0	35.0	39.0	32.0	39.0
13	36.66234750081044	39.0	35.0	39.0	32.0	39.0
14	37.65142300644599	39.0	36.0	41.0	32.0	41.0
15	37.667081786850986	39.0	36.0	41.0	32.0	41.0
16	37.535625550353565	39.0	36.0	41.0	32.0	41.0
17	37.54524203294956	39.0	36.0	41.0	32.0	41.0
18	37.52342753112826	39.0	36.0	41.0	32.0	41.0
19	37.54831558279425	39.0	36.0	41.0	32.0	41.0
20	37.46347414976361	39.0	36.0	41.0	32.0	41.0
21	37.38083164028163	39.0	36.0	41.0	31.0	41.0
22	37.294408216160875	39.0	36.0	41.0	31.0	41.0
23	37.09415538880923	39.0	36.0	40.0	31.0	41.0
24	36.94055127705019	39.0	36.0	40.0	31.0	41.0
25	36.8470821777026	39.0	36.0	40.0	30.0	41.0
26	36.938108646021014	39.0	36.0	40.0	31.0	41.0
27	36.83210528009883	39.0	35.0	40.0	30.0	41.0
28	36.72534684249369	39.0	35.0	40.0	30.0	41.0
29	36.62204092752921	39.0	35.0	40.0	30.0	41.0
30	36.4526757242059	39.0	35.0	40.0	30.0	41.0
31	36.304622855010145	38.0	35.0	40.0	30.0	41.0
32	36.17042414298118	38.0	35.0	40.0	30.0	41.0
33	35.97898674399909	38.0	35.0	40.0	29.0	41.0
34	35.85567056726365	38.0	35.0	40.0	29.0	41.0
35	35.641374287941154	38.0	34.0	40.0	27.0	41.0
36	35.54023721627813	38.0	34.0	40.0	27.0	41.0
37	35.39926393443503	38.0	34.0	40.0	26.0	41.0
38	35.280166656065134	38.0	34.0	40.0	25.0	41.0
39	35.19570496220618	38.0	34.0	40.0	25.0	41.0
40	34.99154093634253	38.0	34.0	40.0	24.0	41.0
41	34.85924954189891	38.0	33.0	40.0	24.0	41.0
42	34.659815395415386	38.0	33.0	40.0	23.0	41.0
43	34.33457971572059	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	13.0
13	19.0
14	27.0
15	42.0
16	85.0
17	230.0
18	457.0
19	964.0
20	2173.0
21	4219.0
22	7401.0
23	12680.0
24	20566.0
25	31509.0
26	46448.0
27	64746.0
28	88649.0
29	117288.0
30	148760.0
31	185114.0
32	227857.0
33	279572.0
34	343528.0
35	419618.0
36	530813.0
37	709312.0
38	945374.0
39	1031901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.649583896300165	22.52694385454802	13.613975014618617	23.209497234533195
2	18.071062955466672	22.54194565936285	35.97109000852976	23.415901376640715
3	19.676447664584934	25.490576260898823	31.963079083077428	22.869896991438818
4	13.283398845684882	15.595171219832576	35.35957965824241	35.76185027624013
5	11.656670572628277	40.314869298452	34.43301224745046	13.595447881469264
6	32.189792181894674	36.44861872271223	15.35360959134547	16.007979504047615
7	26.019452148649304	34.323458837576624	20.968633774331472	18.6884552394426
8	28.087996027108243	33.828284322328436	19.644259884139316	18.439459766424008
9	25.757447447700603	13.9197780882451	22.614253209006755	37.70852125504754
10	16.357849181855595	27.878411425742406	33.28881329018127	22.474926102220728
11	32.65367940817401	21.78396174865482	24.292558568348834	21.269800274822334
12	20.467941353864028	27.07821937198575	31.253664233934657	21.200175040215566
13	31.056705672636475	20.4887101360087	27.867797121952602	20.586787069402217
14	21.156185073606558	22.16837581226247	27.55467516015337	29.120763953977608
15	23.50160134207717	28.96413591520206	25.506555194762893	22.027707547957878
16	22.716372774349097	28.23910616066454	25.962299678965206	23.082221386021153
17	20.495032735739088	27.050725642855117	27.151925557327587	25.302316064078205
18	21.585432117120604	26.2841966428145	30.64587080591305	21.484500434151848
19	20.965702387183747	24.79131589011092	31.575771951108294	22.667209771597044
20	24.903494136842514	24.02605141001638	30.357138751558615	20.713315701582488
21	24.18289020211627	24.933057080430366	29.422754308372735	21.46129840908063
22	22.794619735860945	24.557073149796565	30.425231234715593	22.223075879626897
23	21.974731825974466	26.073481637254442	31.216705764601567	20.735080772169525
24	20.803748037120172	25.358376448354324	29.702519766745883	24.13535574777962
25	22.012016004990638	26.225511421680615	30.490545605869823	21.27192696745892
26	23.12647958413388	25.423997369798514	29.425110913726787	22.024412132340824
27	21.928557688549503	26.65012189205904	29.13691532237978	22.28440509701167
28	21.898669035278573	26.052157232709224	29.31831645646258	22.730857275549628
29	20.794015065414	25.970078392572898	30.366526854188585	22.869379687824512
30	20.36676059878468	26.376123411015733	31.878432884262704	21.378683105936883
31	21.825978297772224	25.736851100094032	30.72570799705405	21.711462605079692
32	22.726508093310844	25.68839699488751	29.081544676256073	22.503550235545582
33	23.340394208345373	25.76294619352673	28.799441771921984	22.097217826205913
34	21.996631012313358	27.99112613548143	28.828429933716166	21.183812918489046
35	20.793440283620328	28.849773497654507	29.19307150362151	21.16371471510366
36	21.600568037687292	25.71935857417329	30.872124079295364	21.80794930884405
37	21.75752178614592	25.88112133030564	30.24777693561601	22.113579947932433
38	21.733744979281035	25.370849213276998	31.171470437439602	21.723935370002366
39	22.765382501956175	24.863968308831026	30.973917934954624	21.396731254258174
40	20.980857467143558	25.611318756356127	30.403925989563497	23.003897786936818
41	21.82185902825091	25.208530834744103	30.442302253987645	22.527307883017343
42	19.896090947340024	26.805159701205433	30.6188752210036	22.679874130450944
43	20.333710645648555	26.436360542992528	30.25758654489467	22.972342266464242
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	333.0
1	507.0
2	681.0
3	1416.5
4	2152.0
5	2152.0
6	4001.0
7	5850.0
8	6563.5
9	7277.0
10	11897.0
11	16517.0
12	16517.0
13	31808.0
14	47099.0
15	75474.5
16	103850.0
17	99754.5
18	95659.0
19	95659.0
20	105817.0
21	115975.0
22	96315.5
23	76656.0
24	86323.5
25	95991.0
26	95991.0
27	106094.0
28	116197.0
29	124034.0
30	131871.0
31	142226.5
32	152582.0
33	152582.0
34	168351.5
35	184121.0
36	203173.0
37	222225.0
38	234808.5
39	247392.0
40	247392.0
41	262614.5
42	277837.0
43	310751.5
44	343666.0
45	422445.5
46	501225.0
47	501225.0
48	508671.5
49	516118.0
50	478555.5
51	440993.0
52	419953.0
53	398913.0
54	398913.0
55	351701.0
56	304489.0
57	261087.0
58	217685.0
59	194213.0
60	170741.0
61	170741.0
62	151455.5
63	132170.0
64	115974.0
65	99778.0
66	84458.5
67	69139.0
68	69139.0
69	58731.5
70	48324.0
71	40448.5
72	32573.0
73	25951.0
74	19329.0
75	19329.0
76	14755.5
77	10182.0
78	7876.0
79	5570.0
80	4485.5
81	3401.0
82	3401.0
83	2564.0
84	1727.0
85	1380.5
86	1034.0
87	879.0
88	724.0
89	724.0
90	783.0
91	842.0
92	539.5
93	237.0
94	194.5
95	152.0
96	152.0
97	100.5
98	49.0
99	47.5
100	46.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5219372.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.365254579469365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.24698929813992	26.24796638566258
2	14.053955538358126	10.502592530785895
3	5.977316205944741	6.700318252113395
4	3.1044870090844414	4.6399978973238225
5	1.7706297099267676	3.3080014938692837
6	1.0450045103312262	2.3428115739131994
7	0.7483138363491997	1.9572655900369056
8	0.5221512656735484	1.5608251976707423
9	0.39189283535578084	1.3178858004844942
>10	1.8274777947208931	12.477646807797171
>50	0.16270949564049872	4.221347280715708
>100	0.12943739048197253	9.415260885784793
>500	0.011565183360831014	2.907065337289612
>1k	0.006784906467708201	4.968315034812317
>5k	5.654088723089834E-4	1.3389684402534545
>10k+	7.196112920296153E-4	6.093731491486841
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	51551	0.9876858748523769	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	51496	0.9866321082306454	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	41559	0.7962452187734463	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	33055	0.6333137396606335	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19444	0.37253523987177	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	17458	0.33448468513070156	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	16815	0.32216519535300414	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	15411	0.29526540740916724	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	14012	0.26846141643094223	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	12767	0.24460797199356552	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	12508	0.23964568917486628	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	10660	0.20423913068468774	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	10366	0.1986062691067048	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	10180	0.19504262198594008	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	8655	0.16582454747429384	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	8168	0.15649392302368945	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	6724	0.12882775935495688	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	6548	0.12545570616541607	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	6466	0.12388463592938001	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	6125	0.11735128287464469	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6089	0.11666154472223861	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5679	0.10880619354205832	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	5230	0.10020362603010477	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.915939312239097E-5	0.0	0.0	0.0	0.0
3	1.915939312239097E-5	0.0	0.0	1.915939312239097E-5	0.0
4	1.915939312239097E-5	0.0	0.0	1.915939312239097E-5	0.0
5	1.915939312239097E-5	0.0	0.0	1.915939312239097E-5	0.0
6	5.747817936717291E-5	0.0	0.0	1.915939312239097E-5	0.0
7	5.747817936717291E-5	0.0	0.0	1.915939312239097E-5	0.0
8	7.663757248956388E-5	0.0	0.0	1.915939312239097E-5	0.0
9	7.663757248956388E-5	0.0	0.0	1.915939312239097E-5	0.0
10	7.663757248956388E-5	1.915939312239097E-5	0.0	1.1495635873434582E-4	0.0
11	7.663757248956388E-5	1.915939312239097E-5	0.0	1.915939312239097E-4	0.0
12	7.663757248956388E-5	1.915939312239097E-5	0.0	3.0655028995825554E-4	0.0
13	7.663757248956388E-5	1.915939312239097E-5	0.0	3.4486907620303746E-4	0.0
14	9.579696561195485E-5	1.915939312239097E-5	0.0	4.406660418149923E-4	0.0
15	9.579696561195485E-5	1.915939312239097E-5	0.0	7.472163317732479E-4	0.0
16	9.579696561195485E-5	1.915939312239097E-5	0.0	0.001149563587343458	0.0
17	9.579696561195485E-5	1.915939312239097E-5	0.0	0.001877620525994315	0.0
18	9.579696561195485E-5	1.915939312239097E-5	0.0	0.002069214457218225	0.0
19	9.579696561195485E-5	1.915939312239097E-5	0.0	0.002471561712788435	0.0
20	9.579696561195485E-5	1.915939312239097E-5	0.0	0.003276256223928856	0.0
21	9.579696561195485E-5	1.915939312239097E-5	0.0	0.004694051314985788	0.0
22	9.579696561195485E-5	1.915939312239097E-5	0.0	0.00810442329077138	0.0
23	9.579696561195485E-5	1.915939312239097E-5	0.0	0.0142162696968141	0.0
24	1.1495635873434582E-4	1.915939312239097E-5	0.0	0.023240343857460245	0.0
25	1.1495635873434582E-4	1.915939312239097E-5	0.0	0.02732129459252952	0.0
26	1.1495635873434582E-4	1.915939312239097E-5	0.0	0.032915837384267684	0.0
27	1.1495635873434582E-4	1.915939312239097E-5	0.0	0.06450967664309039	0.0
28	1.7243453810151873E-4	1.915939312239097E-5	0.0	0.12438278015056217	0.0
29	1.7243453810151873E-4	1.915939312239097E-5	0.0	0.20268721984177407	0.0
30	2.6823150371347356E-4	1.915939312239097E-5	0.0	0.3502528656704293	0.0
31	3.4486907620303746E-4	1.915939312239097E-5	0.0	0.556695326564192	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12505	0.0	29.085165	1
ATTGGAC	2110	0.0	20.954975	3
GTATTGG	2005	0.0	20.852867	1
GTATCAA	20565	0.0	17.667883	2
TTGGACC	3330	0.0	17.61111	4
TATTGGA	2670	0.0	16.837078	2
TGGACCC	3305	0.0	16.792738	5
GGACCCT	3210	0.0	16.771029	6
ACGTTTA	350	0.0	15.857143	26
TATACTG	645	0.0	14.914728	5
GACCCTC	3590	0.0	14.892758	7
TCACGTT	420	0.0	13.654761	24
TATACCG	340	0.0	13.602941	5
CGTCTGT	1305	0.0	13.325671	34
ACGGACC	585	0.0	13.282051	8
CGACGAG	295	0.0	13.169492	24
CTGTGCG	380	0.0	13.144737	9
TACGTTA	240	8.185452E-11	13.104167	19
ACCCTCG	3985	0.0	13.091594	8
ATAACGC	610	0.0	12.737704	3
>>END_MODULE