FastQCFastQC Report
Wed 25 May 2016
SRR2088140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663601
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67111.0113004651891724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64260.9683529711377771No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53470.8057552655888102No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG40560.6112106521840684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26480.3990349622740171No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA23730.35759439783846014No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT20760.3128385882480587No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA19110.2879742495867246No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT18900.28480969739346385No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG15820.2383962652256401No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA15820.2383962652256401No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT14060.21187430398688367No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT13290.20027094594492773No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA12820.1931883767504871No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA10870.16380324924163767No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA9930.1496381108527564No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC9220.13893891058030353No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT8160.12296545665241614No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA7960.11995159742073927No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT7940.11965021149757159No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG7740.11663635226589472No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC7710.11618427338114319No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.11603358041955933No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG7050.10623853791660953No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.10473160830077109No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.10412883645443571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.027.8318561
AAGACCG400.001930331123.1250025
ATTGGAC3150.022.904763
GTATTGG2850.022.71931
ATTACGT502.700435E-422.19999917
GTGTTAC450.003824129220.5555571
CTGTGCG450.003824129220.5555579
ACGTTAA555.139947E-420.18181820
AATTACG555.139947E-420.18181816
CCGCTAG656.8965135E-519.92307736
CTTAACG609.2312077E-418.530
CACCTCG500.007031826318.49999831
ACCGTGG500.007031826318.4999988
TAGTACT1002.8703835E-718.4999984
TAGCACT852.7203074E-517.4117644
TATTGGA3850.017.29872
TACCCTA752.0656086E-417.2666665
GCCGCTA752.0656086E-417.26666635
TAAACAG1401.8644641E-917.1785725
TTGGACC4850.017.164954
Produced by FastQC (version 0.11.5)