##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	663601
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.580805333325298	31.0	31.0	34.0	30.0	34.0
2	31.759245389925574	31.0	31.0	34.0	30.0	34.0
3	31.85426332992265	31.0	31.0	34.0	30.0	34.0
4	35.58130412702814	37.0	35.0	37.0	33.0	37.0
5	35.46501436857389	37.0	35.0	37.0	33.0	37.0
6	35.4939293340426	37.0	35.0	37.0	33.0	37.0
7	35.3573321920853	37.0	35.0	37.0	33.0	37.0
8	35.38213474663239	37.0	35.0	37.0	33.0	37.0
9	36.90221684415786	39.0	37.0	39.0	33.0	39.0
10	36.76655399856239	39.0	35.0	39.0	32.0	39.0
11	36.847287752730935	39.0	37.0	39.0	33.0	39.0
12	36.727721929291846	39.0	35.0	39.0	32.0	39.0
13	36.74139128783712	39.0	35.0	39.0	32.0	39.0
14	37.744358432250706	39.0	37.0	41.0	32.0	41.0
15	37.75622851683467	39.0	37.0	41.0	32.0	41.0
16	37.63214793226653	39.0	36.0	41.0	32.0	41.0
17	37.64977448798299	39.0	37.0	41.0	32.0	41.0
18	37.631894768091065	39.0	36.0	41.0	32.0	41.0
19	37.6646463763617	39.0	37.0	41.0	32.0	41.0
20	37.589553059745235	39.0	36.0	41.0	32.0	41.0
21	37.50469634614776	39.0	36.0	41.0	32.0	41.0
22	37.41716332555255	39.0	36.0	41.0	32.0	41.0
23	37.20184870125271	39.0	36.0	41.0	31.0	41.0
24	37.05548062766633	39.0	36.0	41.0	31.0	41.0
25	36.95736444037908	39.0	36.0	40.0	31.0	41.0
26	37.05286459785323	39.0	36.0	41.0	31.0	41.0
27	36.97868297365435	39.0	36.0	41.0	31.0	41.0
28	36.88401916211699	39.0	35.0	41.0	30.0	41.0
29	36.79012689854295	39.0	35.0	40.0	30.0	41.0
30	36.62281853101487	39.0	35.0	40.0	30.0	41.0
31	36.478600845990286	39.0	35.0	40.0	30.0	41.0
32	36.332259897136986	38.0	35.0	40.0	30.0	41.0
33	36.157367152852395	38.0	35.0	40.0	30.0	41.0
34	36.01038425198274	38.0	35.0	40.0	29.0	41.0
35	35.81667598451479	38.0	35.0	40.0	28.0	41.0
36	35.71195492472133	38.0	35.0	40.0	27.0	41.0
37	35.57586863190381	38.0	34.0	40.0	27.0	41.0
38	35.47079344365063	38.0	34.0	40.0	26.0	41.0
39	35.38528422952949	38.0	34.0	40.0	26.0	41.0
40	35.18741080860336	38.0	34.0	40.0	25.0	41.0
41	35.06240647618072	38.0	34.0	40.0	24.0	41.0
42	34.87560296021254	38.0	34.0	40.0	24.0	41.0
43	34.553100432338105	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	0.0
15	1.0
16	2.0
17	22.0
18	52.0
19	99.0
20	209.0
21	429.0
22	849.0
23	1391.0
24	2257.0
25	3557.0
26	5312.0
27	7441.0
28	10117.0
29	13925.0
30	17742.0
31	22503.0
32	27981.0
33	34499.0
34	42946.0
35	53468.0
36	68188.0
37	92722.0
38	123822.0
39	134062.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.594875535148375	22.77226827566565	13.656851029458966	22.976005159727006
2	17.63047373346333	22.579230591876744	36.63195203141647	23.158343643243455
3	19.39885563764973	26.090376596780295	32.031446607223316	22.479321158346657
4	13.259473689762372	15.778005156713146	35.820922512172224	35.14159864135225
5	11.337083578837284	40.5219401417418	34.94283462502317	13.19814165439775
6	31.691784671813334	36.80253646392938	15.59702290985095	15.908655954406337
7	25.29788231181086	34.55555371375269	21.626549688743687	18.520014285692756
8	27.475696992620563	34.56715707179465	19.962748699896473	17.99439723568831
9	25.395531350917196	13.935030236542742	22.876849191004837	37.79258922153523
10	16.150970236633157	27.93998200726039	33.755524780704064	22.15352297540239
11	32.15200097649039	21.97359557927128	24.496949220992736	21.377454223245596
12	19.726763522056174	27.30059177126014	31.983224859516486	20.9894198471672
13	30.704143001592826	21.228418884239176	28.108908817195875	19.958529296972127
14	20.743790319785536	22.194511460953194	27.860717509467285	29.20098070979399
15	23.103190019303767	29.243325431999047	25.933957302656264	21.719527246040922
16	22.17235959560037	28.63527933200824	26.26261865187063	22.929742420520764
17	19.88107311471803	27.23036885116207	27.59278542377121	25.29577261034869
18	20.983392128703844	26.937421733843074	30.938018477970953	21.14116765948213
19	20.401114525143875	25.350172769480455	31.86809543686643	22.38061726850924
20	24.50267555353292	24.499360308378076	30.698718054975803	20.299246083113196
21	23.81024139505516	25.308882898006484	29.564452133134218	21.316423573804137
22	22.305120094755733	24.897340419921008	30.66044204273351	22.13709744258975
23	21.316724959727306	26.63091224998154	31.735636323634232	20.316726466656924
24	20.43381489780757	25.727206559363232	30.203390290249715	23.63558825257949
25	21.458979115462455	26.589471685545984	30.864932391602785	21.08661680738878
26	22.77588490674366	25.816266099659284	29.77557297231318	21.632276021283875
27	21.47058247350441	27.131363575401483	29.466200322181553	21.931853628912553
28	21.33480811511737	26.391009055140064	29.793656127703244	22.480526702039327
29	20.512627316715918	26.22750719182159	30.850767253213906	22.409098238248585
30	19.960337612511132	26.55842893545971	32.13195881259974	21.349274639429417
31	21.10665897127943	25.94360165219763	31.495733128792754	21.454006247730188
32	21.984746858428483	26.075608686545078	29.731419934569114	22.208224520457325
33	22.850779308650832	26.090979368626634	29.40426551497059	21.653975807751948
34	21.270311527559482	28.312495008295645	29.56294520351838	20.85424826062649
35	20.422211539765613	28.982626608459	29.656374839700362	20.93878701207503
36	21.038244366720363	25.895379904490802	31.396125081185833	21.670250647603005
37	21.104097190932503	26.151256553260165	30.821984897551392	21.92266135825594
38	21.186375547957283	25.66029888442001	31.685907646311563	21.46741792131115
39	22.11358934058267	25.04064942638724	31.76532283706625	21.080438395963842
40	20.70551430754324	25.768496430837207	30.864329619756447	22.661659641863107
41	21.50509116170711	25.35981711902182	30.726596252868816	22.40849546640225
42	19.379566938567	26.996644067745528	30.974636867635823	22.64915212605165
43	20.015641929412403	26.52768757129661	30.626234740453977	22.830435758837012
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	58.0
2	81.0
3	179.5
4	278.0
5	278.0
6	517.5
7	757.0
8	833.0
9	909.0
10	1569.5
11	2230.0
12	2230.0
13	4288.5
14	6347.0
15	9773.5
16	13200.0
17	12849.5
18	12499.0
19	12499.0
20	13781.5
21	15064.0
22	12629.5
23	10195.0
24	11491.0
25	12787.0
26	12787.0
27	14426.5
28	16066.0
29	17336.0
30	18606.0
31	20110.5
32	21615.0
33	21615.0
34	23662.0
35	25709.0
36	27744.0
37	29779.0
38	31738.5
39	33698.0
40	33698.0
41	35259.5
42	36821.0
43	41176.0
44	45531.0
45	55465.0
46	65399.0
47	65399.0
48	65868.0
49	66337.0
50	60688.5
51	55040.0
52	51715.0
53	48390.0
54	48390.0
55	42381.5
56	36373.0
57	30530.0
58	24687.0
59	21893.5
60	19100.0
61	19100.0
62	16658.5
63	14217.0
64	12326.0
65	10435.0
66	8907.5
67	7380.0
68	7380.0
69	6223.0
70	5066.0
71	4354.5
72	3643.0
73	2964.0
74	2285.0
75	2285.0
76	1781.5
77	1278.0
78	1018.0
79	758.0
80	603.5
81	449.0
82	449.0
83	330.5
84	212.0
85	178.5
86	145.0
87	114.5
88	84.0
89	84.0
90	78.0
91	72.0
92	49.0
93	26.0
94	19.0
95	12.0
96	12.0
97	7.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	663601.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.48726398457789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.21152756529045	46.43904260155964
2	10.37619720968419	11.722459818789426
3	3.0454846739394292	5.16093290213408
4	1.2869507786113825	2.9078531346631693
5	0.7165613166368722	2.0238294127001852
6	0.453357940507972	1.5365369798987025
7	0.3097685218302364	1.224858338671593
8	0.23439069954305325	1.0592071456494663
9	0.17473891882763312	0.8883471092576615
>10	1.0649915177192433	11.20463877670182
>50	0.0767958923889878	2.990491885789777
>100	0.04013719974510884	4.521482349111143
>500	0.005084045301039362	2.01648378750565
>1k	0.003210975979603808	3.5099907438315125
>5k	8.02743994900952E-4	2.7938450137361843
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6711	1.0113004651891724	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6426	0.9683529711377771	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5347	0.8057552655888102	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4056	0.6112106521840684	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2648	0.3990349622740171	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2373	0.35759439783846014	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2076	0.3128385882480587	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1911	0.2879742495867246	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1890	0.28480969739346385	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1582	0.2383962652256401	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1582	0.2383962652256401	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1406	0.21187430398688367	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1329	0.20027094594492773	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1282	0.1931883767504871	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1087	0.16380324924163767	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	993	0.1496381108527564	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	922	0.13893891058030353	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	816	0.12296545665241614	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	796	0.11995159742073927	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	794	0.11965021149757159	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	774	0.11663635226589472	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	771	0.11618427338114319	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	770	0.11603358041955933	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	705	0.10623853791660953	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	695	0.10473160830077109	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	691	0.10412883645443571	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	1.506929615838433E-4	0.0
7	0.0	0.0	0.0	1.506929615838433E-4	0.0
8	0.0	0.0	0.0	1.506929615838433E-4	0.0
9	0.0	0.0	0.0	4.520788847515299E-4	0.0
10	0.0	0.0	0.0	7.534648079192165E-4	0.0
11	0.0	0.0	0.0	7.534648079192165E-4	0.0
12	0.0	0.0	0.0	0.0012055436926707464	0.0
13	0.0	0.0	0.0	0.0013562366542545898	0.0
14	0.0	0.0	0.0	0.001506929615838433	0.0
15	0.0	0.0	0.0	0.002561780346925336	0.0
16	0.0	0.0	0.0	0.0036166310780122394	0.0
17	0.0	0.0	0.0	0.0061784114249375756	0.0
18	0.0	0.0	0.0	0.006781183271272949	0.0
19	0.0	0.0	0.0	0.008589498810279069	0.0
20	0.0	0.0	0.0	0.010397814349285188	0.0
21	0.0	0.0	0.0	0.014315831350465115	0.0
22	0.0	0.0	0.0	0.024562952738166458	0.0
23	0.0	0.0	0.0	0.03797462631912851	0.0
24	0.0	0.0	0.0	0.05861956205611504	0.0
25	0.0	0.0	0.0	0.06690767494322643	0.0
26	0.0	0.0	0.0	0.07474370894558628	0.0
27	0.0	0.0	0.0	0.11949951853598774	0.0
28	0.0	0.0	0.0	0.19831193744433778	0.0
29	0.0	0.0	0.0	0.29249503843423985	0.0
30	0.0	0.0	0.0	0.4638329357550697	0.0
31	0.0	0.0	0.0	0.6796252567431333	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1695	0.0	27.831856	1
AAGACCG	40	0.0019303311	23.125002	5
ATTGGAC	315	0.0	22.90476	3
GTATTGG	285	0.0	22.7193	1
ATTACGT	50	2.700435E-4	22.199999	17
GTGTTAC	45	0.0038241292	20.555557	1
CTGTGCG	45	0.0038241292	20.555557	9
ACGTTAA	55	5.139947E-4	20.181818	20
AATTACG	55	5.139947E-4	20.181818	16
CCGCTAG	65	6.8965135E-5	19.923077	36
CTTAACG	60	9.2312077E-4	18.5	30
CACCTCG	50	0.0070318263	18.499998	31
ACCGTGG	50	0.0070318263	18.499998	8
TAGTACT	100	2.8703835E-7	18.499998	4
TAGCACT	85	2.7203074E-5	17.411764	4
TATTGGA	385	0.0	17.2987	2
TACCCTA	75	2.0656086E-4	17.266666	5
GCCGCTA	75	2.0656086E-4	17.266666	35
TAAACAG	140	1.8644641E-9	17.178572	5
TTGGACC	485	0.0	17.16495	4
>>END_MODULE