FastQCFastQC Report
Wed 25 May 2016
SRR2088139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909876
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT131881.4494282737427957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT120051.3194105570429377No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93611.0288215097441848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48390.5318307109979822No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG40170.44148873033248487No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT23960.26333258597874876No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT23890.2625632503769744No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA20710.22761343303922735No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT18870.20739089722115978No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT17170.1887070326066409No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG16810.1847504495118016No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA16310.1792551952134137No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.17650756806421974No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT14060.15452655087066808No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.1511194932056676No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA13460.1479322457126026No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12370.13595259134211696No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA12090.13287524893501973No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11750.12913847601211595No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC9980.10968527579582274No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9430.10364049606759604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAC509.08837E-625.90000228
GGTATCA36400.023.6840651
CCGTTTA551.9020943E-523.54545427
TTACGGC652.6818252E-622.7692318
ACCGTTA652.6818252E-622.769238
GTATACG555.141963E-420.1818181
GACCGTT759.262292E-619.7333347
ATCACGT851.2447235E-619.58823623
AACCGTG609.234802E-418.57
AATTACG500.007033783518.516
CCGCCGT701.2188429E-418.524
TTCTGCG500.007033783518.52
CTAGACA852.722105E-517.4117644
TAAACGT852.722105E-517.4117644
AACGTGA752.06667E-417.2666666
CGCCGTT752.06667E-417.26666625
TGTTACG650.001579591517.07692316
ACGTTTA904.444317E-516.44444526
GCTGCGT700.002591936815.8571438
ACGGCTC700.002591936815.8571435
Produced by FastQC (version 0.11.5)