##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088139_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	909876
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57441013940361	31.0	31.0	34.0	30.0	34.0
2	31.750156065222075	31.0	31.0	34.0	30.0	34.0
3	31.825803735893683	31.0	31.0	34.0	30.0	34.0
4	35.54669207672254	37.0	35.0	37.0	33.0	37.0
5	35.43999841736676	37.0	35.0	37.0	33.0	37.0
6	35.47304687671727	37.0	35.0	37.0	33.0	37.0
7	35.33405870690072	37.0	35.0	37.0	33.0	37.0
8	35.37326185106542	37.0	35.0	37.0	33.0	37.0
9	36.88647024429702	39.0	37.0	39.0	33.0	39.0
10	36.74829757021836	39.0	35.0	39.0	32.0	39.0
11	36.828362326295014	39.0	37.0	39.0	32.0	39.0
12	36.71294440121511	39.0	35.0	39.0	32.0	39.0
13	36.739426031679045	39.0	35.0	39.0	32.0	39.0
14	37.70458062417296	39.0	37.0	41.0	32.0	41.0
15	37.72335900716141	39.0	37.0	41.0	32.0	41.0
16	37.60241285625734	39.0	36.0	41.0	32.0	41.0
17	37.651839371518754	39.0	37.0	41.0	32.0	41.0
18	37.65407813811992	39.0	37.0	41.0	32.0	41.0
19	37.693079056926436	39.0	37.0	41.0	32.0	41.0
20	37.622500208819666	39.0	37.0	41.0	32.0	41.0
21	37.55648352083141	39.0	36.0	41.0	32.0	41.0
22	37.46755052336802	39.0	36.0	41.0	32.0	41.0
23	37.27504846814291	39.0	36.0	41.0	31.0	41.0
24	37.13623834456563	39.0	36.0	41.0	31.0	41.0
25	37.03564221937934	39.0	36.0	40.0	31.0	41.0
26	37.09851122570548	39.0	36.0	41.0	31.0	41.0
27	36.98734333029995	39.0	36.0	41.0	31.0	41.0
28	36.88503158672171	39.0	35.0	41.0	31.0	41.0
29	36.76786177457148	39.0	35.0	40.0	30.0	41.0
30	36.575146503479594	39.0	35.0	40.0	30.0	41.0
31	36.39161819852375	38.0	35.0	40.0	30.0	41.0
32	36.211234278077455	38.0	35.0	40.0	30.0	41.0
33	36.00001868386462	38.0	35.0	40.0	30.0	41.0
34	35.86472442398745	38.0	35.0	40.0	29.0	41.0
35	35.665469800280476	38.0	35.0	40.0	28.0	41.0
36	35.53067231139188	38.0	34.0	40.0	27.0	41.0
37	35.37059005842554	38.0	34.0	40.0	26.0	41.0
38	35.20945601378649	38.0	34.0	40.0	25.0	41.0
39	35.07602354606562	38.0	34.0	40.0	25.0	41.0
40	34.8519941178798	38.0	34.0	40.0	24.0	41.0
41	34.67607893822895	38.0	33.0	40.0	23.0	41.0
42	34.45785359763308	38.0	33.0	40.0	22.0	41.0
43	34.08526436569379	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	7.0
16	10.0
17	29.0
18	59.0
19	160.0
20	331.0
21	607.0
22	1234.0
23	2051.0
24	3359.0
25	5056.0
26	7695.0
27	10746.0
28	14969.0
29	19513.0
30	25321.0
31	31579.0
32	38373.0
33	48618.0
34	59643.0
35	73776.0
36	94592.0
37	128474.0
38	163416.0
39	180255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.65213281809829	22.775740870184507	14.499008656124571	22.07311765559263
2	18.206766636332862	23.52968975992333	35.47186649609397	22.791677107649832
3	19.7644514197539	25.771973323837532	32.40287687553029	22.060698380878275
4	13.58031204252008	16.39586053484211	34.64450100892869	35.37932641370912
5	11.418479001534275	41.0321846053748	33.81592656581776	13.733409827273166
6	31.365812484338523	36.497830473603	15.485956328115039	16.65040071394344
7	24.814700025058357	35.41020974286606	21.110019387257164	18.665070844818416
8	28.19296255753531	33.910225129578095	19.700926280064536	18.195886032822056
9	25.787579846044956	13.9313488871011	23.209426339413284	37.07164492744066
10	16.86966136044912	29.167161239553526	32.34748471220254	21.615692687794823
11	31.925559087172317	22.90509915636856	23.544087326185107	21.625254430274016
12	20.10482747099605	28.973178762820428	30.841235509014414	20.08075825716911
13	30.900364445265073	21.69614321072322	27.815438587236063	19.588053756775647
14	20.66853065692468	23.58211448592995	28.894486721267516	26.854868135877858
15	23.631681679701412	29.662283651838273	26.138177070282104	20.567857598178215
16	21.66624902733999	28.50839015426278	27.147875095067896	22.677485723329333
17	19.58025049567194	28.411673678611148	28.070858007025134	23.937217818691778
18	20.162417736043153	27.138862878018543	31.79697013659004	20.90174924934826
19	20.05427113145088	26.25841323433083	33.301021238058816	20.386294396159478
20	23.489684308631066	25.030773424070972	32.08722946863089	19.39231279866707
21	22.873666301781782	25.954745481801915	30.864315577067647	20.307272639348657
22	21.84319621574808	25.620084495030092	31.651016182424858	20.88570310679697
23	20.73865010177211	27.24206375374227	32.2085646835393	19.810721460946326
24	19.44385828398595	27.002470666332556	31.24161973719496	22.312051312486535
25	20.66314530771226	27.500010990508596	31.971389508020877	19.865454193758268
26	21.55634394137223	26.568455481845877	30.949052398348787	20.926148178433106
27	20.575441049109987	27.73169091172863	30.724186592458754	20.96868144670263
28	20.051083883957816	27.66332994825669	30.82046344776651	21.465122720018993
29	19.2913100246627	27.378675775600193	31.983149352219424	21.346864847517683
30	19.0666640289446	27.66212099231104	33.268049712268486	20.00316526647587
31	20.17868368876638	27.365157450026157	32.004141223639266	20.452017637568197
32	20.925159032659398	27.701137297829593	30.905639889391523	20.46806378011949
33	21.488202788072222	27.45374094931617	30.367654493579344	20.690401769032263
34	20.177035112476865	28.92833748774558	30.743090267245204	20.151537132532347
35	19.267130905749795	29.384883214855652	30.951580215326047	20.39640566406851
36	19.620146041878233	26.901248082156247	32.63554594252403	20.84305993344148
37	19.708180015738407	26.97785192707578	32.07074370573573	21.24322435145009
38	19.973710703436513	26.788265653781394	32.55949162303435	20.678532019747745
39	20.70040313185533	26.328972299522135	32.68972914990614	20.280895418716398
40	19.409348086992072	26.605603400902982	32.260989409545914	21.72405910255903
41	19.689606056209858	26.28797770245616	32.24725127379995	21.77516496753404
42	18.041799102295258	27.465720603686655	32.22614949729414	22.26633079672395
43	18.629241786792925	26.997524937464007	32.14141267601299	22.231820599730074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	113.0
1	177.0
2	241.0
3	443.0
4	645.0
5	645.0
6	1195.0
7	1745.0
8	1951.0
9	2157.0
10	3489.0
11	4821.0
12	4821.0
13	8770.0
14	12719.0
15	20197.0
16	27675.0
17	25264.5
18	22854.0
19	22854.0
20	24531.5
21	26209.0
22	21023.5
23	15838.0
24	17699.0
25	19560.0
26	19560.0
27	21628.5
28	23697.0
29	24680.0
30	25663.0
31	27822.5
32	29982.0
33	29982.0
34	32693.5
35	35405.0
36	38382.0
37	41359.0
38	43531.5
39	45704.0
40	45704.0
41	47663.5
42	49623.0
43	55287.5
44	60952.0
45	72879.0
46	84806.0
47	84806.0
48	85764.0
49	86722.0
50	77979.0
51	69236.0
52	62882.5
53	56529.0
54	56529.0
55	51013.5
56	45498.0
57	39651.0
58	33804.0
59	30045.0
60	26286.0
61	26286.0
62	22886.0
63	19486.0
64	16686.0
65	13886.0
66	11712.0
67	9538.0
68	9538.0
69	8027.0
70	6516.0
71	5449.0
72	4382.0
73	3532.5
74	2683.0
75	2683.0
76	2109.0
77	1535.0
78	1209.0
79	883.0
80	715.0
81	547.0
82	547.0
83	397.5
84	248.0
85	193.5
86	139.0
87	113.5
88	88.0
89	88.0
90	77.5
91	67.0
92	42.0
93	17.0
94	15.0
95	13.0
96	13.0
97	8.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	909876.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.52588322991281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01904865935904	42.03071206050204
2	11.434622004276449	12.012272403496324
3	3.6151924762717136	5.69673533586922
4	1.4957264749507824	3.1425741666861557
5	0.8125676155078637	2.134041584428737
6	0.5087330597000072	1.6032991973399302
7	0.34203151110614977	1.2575855049318574
8	0.23755609434941644	0.9982274937881277
9	0.20123321877096556	0.951295729602891
>10	1.1699562640292946	11.697684290247693
>50	0.09564656322994335	3.4246348041595582
>100	0.057175800937683756	5.460210166546188
>500	0.006516359656582487	2.2443469954161333
>1k	0.0033632824033974127	3.531200157790072
>5k	2.102051502123383E-4	1.0335677780336707
>10k+	4.204103004246766E-4	2.781612331161443
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13188	1.4494282737427957	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12005	1.3194105570429377	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9361	1.0288215097441848	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4839	0.5318307109979822	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4017	0.44148873033248487	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2396	0.26333258597874876	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2389	0.2625632503769744	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2071	0.22761343303922735	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1887	0.20739089722115978	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1717	0.1887070326066409	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1681	0.1847504495118016	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1631	0.1792551952134137	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1606	0.17650756806421974	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1406	0.15452655087066808	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1375	0.1511194932056676	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1346	0.1479322457126026	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1237	0.13595259134211696	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1209	0.13287524893501973	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1175	0.12913847601211595	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	998	0.10968527579582274	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	943	0.10364049606759604	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.198101719355165E-4	0.0
10	0.0	0.0	0.0	3.2971525790327476E-4	0.0
11	0.0	0.0	0.0	3.2971525790327476E-4	0.0
12	0.0	0.0	0.0	5.495254298387912E-4	0.0
13	1.0990508596775825E-4	0.0	0.0	5.495254298387912E-4	0.0
14	1.0990508596775825E-4	0.0	0.0	5.495254298387912E-4	0.0
15	1.0990508596775825E-4	0.0	0.0	7.693356017743077E-4	0.0
16	1.0990508596775825E-4	0.0	0.0	0.001758481375484132	0.0
17	1.0990508596775825E-4	0.0	0.0	0.0025278169772584395	0.0
18	1.0990508596775825E-4	0.0	0.0	0.003516962750968264	0.0
19	1.0990508596775825E-4	0.0	0.0	0.004176393266774814	0.0
20	1.0990508596775825E-4	0.0	0.0	0.004945728868549121	0.0
21	1.0990508596775825E-4	0.0	0.0	0.006814115330001011	0.0
22	1.0990508596775825E-4	0.0	0.0	0.013518325574034264	0.0
23	1.0990508596775825E-4	0.0	0.0	0.025278169772584395	0.0
24	1.0990508596775825E-4	0.0	0.0	0.04066488180807055	0.0
25	1.0990508596775825E-4	0.0	0.0	0.048907763255652416	0.0
26	1.0990508596775825E-4	0.0	0.0	0.06319542443146099	0.0
27	1.0990508596775825E-4	0.0	0.0	0.12825923532437386	0.0
28	1.0990508596775825E-4	0.0	0.0	0.23838413146406764	0.0
29	1.0990508596775825E-4	0.0	0.0	0.37301786177457147	0.0
30	1.0990508596775825E-4	0.0	0.0	0.6184359187405757	0.0
31	1.0990508596775825E-4	0.0	0.0	0.936501237531268	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAC	50	9.08837E-6	25.900002	28
GGTATCA	3640	0.0	23.684065	1
CCGTTTA	55	1.9020943E-5	23.545454	27
TTACGGC	65	2.6818252E-6	22.76923	18
ACCGTTA	65	2.6818252E-6	22.76923	8
GTATACG	55	5.141963E-4	20.181818	1
GACCGTT	75	9.262292E-6	19.733334	7
ATCACGT	85	1.2447235E-6	19.588236	23
AACCGTG	60	9.234802E-4	18.5	7
AATTACG	50	0.0070337835	18.5	16
CCGCCGT	70	1.2188429E-4	18.5	24
TTCTGCG	50	0.0070337835	18.5	2
CTAGACA	85	2.722105E-5	17.411764	4
TAAACGT	85	2.722105E-5	17.411764	4
AACGTGA	75	2.06667E-4	17.266666	6
CGCCGTT	75	2.06667E-4	17.266666	25
TGTTACG	65	0.0015795915	17.076923	16
ACGTTTA	90	4.444317E-5	16.444445	26
GCTGCGT	70	0.0025919368	15.857143	8
ACGGCTC	70	0.0025919368	15.857143	5
>>END_MODULE