FastQCFastQC Report
Wed 25 May 2016
SRR2088138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3482448
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT352861.0132527463439511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT347400.9975741202740143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT281160.8073630963046684No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG186010.5341357573752716No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138050.3964165437646162No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT99910.2868958847339573No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA92420.2653880258944283No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT91350.26231547463163846No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA86450.24824491277400268No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA78900.22656476134029854No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG75380.21645692914869083No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT66860.19199138077582206No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT66460.19084276348132118No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52570.15095702792977814No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA50710.14561595751034906No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA47010.1349912475362159No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT44320.12726679623069748No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42080.12083453938149255No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG36340.10435188120540492No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA36090.10363399539634187No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35310.10139419167206516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79900.029.938051
ATTGGAC9800.018.1224483
GTATCAA135400.017.6665442
GTATTGG11000.017.6590921
TTGGACC16400.016.920734
GGACCCT16050.016.598136
CGAACGT1059.353915E-615.8571444
TAACGGA1403.479545E-815.85714228
TGGACCC17200.015.7034885
CGTCTGT8900.015.58988934
TCGAACG1152.2122815E-514.4782613
AATCGGC1152.2122815E-514.47826125
GACCCTC18400.014.0760877
CGTTTAG1854.9094524E-914.00000126
GTATGCG800.00630200913.87533
TATACCG1601.7865204E-713.8755
TATTGGA14350.013.6655052
TAACGCC3000.013.5666674
CTAACGG1501.3075642E-613.56666727
ACGGACC2601.8189894E-1213.5192318
Produced by FastQC (version 0.11.5)