##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088138_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3482448
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.576942139552408	31.0	31.0	34.0	30.0	34.0
2	31.756647622591924	31.0	31.0	34.0	30.0	34.0
3	31.849691079378644	31.0	31.0	34.0	30.0	34.0
4	35.56438459382595	37.0	35.0	37.0	33.0	37.0
5	35.44952200291289	37.0	35.0	37.0	33.0	37.0
6	35.46631162906094	37.0	35.0	37.0	33.0	37.0
7	35.342702317450254	37.0	35.0	37.0	33.0	37.0
8	35.383794388315344	37.0	35.0	37.0	33.0	37.0
9	36.89368656760991	39.0	37.0	39.0	33.0	39.0
10	36.74829143177443	39.0	35.0	39.0	32.0	39.0
11	36.8106986809279	39.0	37.0	39.0	32.0	39.0
12	36.70296842910504	39.0	35.0	39.0	32.0	39.0
13	36.72136669377404	39.0	35.0	39.0	32.0	39.0
14	37.735603230830726	40.0	37.0	41.0	32.0	41.0
15	37.74585492733847	40.0	37.0	41.0	32.0	41.0
16	37.624216068696505	39.0	36.0	41.0	32.0	41.0
17	37.63160426228906	39.0	36.0	41.0	32.0	41.0
18	37.61148852761046	39.0	36.0	41.0	32.0	41.0
19	37.64200614050806	39.0	37.0	41.0	32.0	41.0
20	37.55504346367842	39.0	36.0	41.0	32.0	41.0
21	37.47325157475431	39.0	36.0	41.0	32.0	41.0
22	37.37014221030723	39.0	36.0	41.0	31.0	41.0
23	37.169199942109685	39.0	36.0	41.0	31.0	41.0
24	37.02397566309676	39.0	36.0	40.0	31.0	41.0
25	36.92024748108227	39.0	36.0	40.0	31.0	41.0
26	37.05367345040041	39.0	36.0	41.0	31.0	41.0
27	36.96039510137696	39.0	36.0	41.0	31.0	41.0
28	36.87456237681079	39.0	35.0	41.0	30.0	41.0
29	36.80300150928313	39.0	35.0	40.0	30.0	41.0
30	36.61916904430446	39.0	35.0	40.0	30.0	41.0
31	36.452803889677604	39.0	35.0	40.0	30.0	41.0
32	36.306143551892234	38.0	35.0	40.0	30.0	41.0
33	36.10784827224987	38.0	35.0	40.0	30.0	41.0
34	35.995016723867806	38.0	35.0	40.0	29.0	41.0
35	35.79254507174264	38.0	35.0	40.0	28.0	41.0
36	35.70883097177617	38.0	35.0	40.0	27.0	41.0
37	35.555914402741976	38.0	34.0	40.0	27.0	41.0
38	35.45563063683937	38.0	34.0	40.0	26.0	41.0
39	35.3607804050484	38.0	34.0	40.0	25.0	41.0
40	35.18032889507611	38.0	34.0	40.0	25.0	41.0
41	35.03750522620869	38.0	34.0	40.0	24.0	41.0
42	34.84686519367985	38.0	33.0	40.0	24.0	41.0
43	34.5313003955838	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	9.0
13	13.0
14	13.0
15	20.0
16	75.0
17	135.0
18	268.0
19	673.0
20	1351.0
21	2598.0
22	4683.0
23	8088.0
24	12998.0
25	20195.0
26	29379.0
27	41336.0
28	55916.0
29	74186.0
30	94287.0
31	118666.0
32	145717.0
33	179360.0
34	221267.0
35	272527.0
36	351100.0
37	475761.0
38	651081.0
39	720743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.32508166668964	22.71359112899891	13.766407998051944	23.194919206259502
2	18.22683353778721	22.724589139593757	35.653080821307306	23.395496501311722
3	19.573271445833505	25.560697532310606	31.769519602302747	23.096511419553142
4	13.512132844481814	15.97987967085223	35.31989565960497	35.188091825060994
5	11.69855228276201	40.32088921356471	34.53317895916895	13.447379544504326
6	32.16091094540393	36.7196294101161	15.273480034734188	15.84597960974579
7	25.851498715845867	34.3681800848139	21.191874221811783	18.58844697752845
8	27.724146921935372	34.36022591004948	19.730086421965236	18.185540746049906
9	25.663785934492054	14.027862009712708	22.453113442038475	37.85523861375676
10	16.366705260207763	28.11189140512651	33.323656232627165	22.197747102038566
11	32.75221912861298	22.080501991702388	23.940343114958214	21.226935764726424
12	20.137788130648325	27.476648610402798	31.259677100706167	21.125886158242707
13	30.786446775371807	21.00737756888258	27.564690126026292	20.641485529719326
14	20.99724102125861	22.285846048526782	27.75403968702476	28.962873243189847
15	23.572498426394304	29.106019673517018	25.446754696696118	21.874727203392556
16	22.550803342935772	28.278699351720398	26.032492086026842	23.138005219316987
17	20.380002802626198	27.38059548915016	27.30694040514029	24.932461303083347
18	21.233683891331616	26.71540249847234	30.519450685265078	21.53146292493097
19	20.978173974169895	25.216772798904678	31.572158435675135	22.232894791250292
20	24.430544260818827	24.552584848359547	30.498000257290276	20.518870633531357
21	23.823471305242748	25.268431861724856	29.427201784491828	21.480895048540567
22	22.550832058368137	24.853292856059873	30.44774825065586	22.14812683491613
23	21.76296674063762	26.318440361492833	31.015251340436382	20.903341557433162
24	20.729268606451555	25.863903782626473	29.79249079957547	23.6143368113465
25	21.769284135757374	26.45773892388343	30.59617832053774	21.17679861982146
26	22.77334794374532	25.67383633581894	29.648339329115608	21.90447639132013
27	21.712140425355955	26.835777590936033	29.28864982334266	22.16343216036535
28	21.5566463591129	26.261153073929606	29.568740150606697	22.613460416350797
29	20.755945243116336	26.219343404409774	30.437468125870076	22.587243226603814
30	20.302873151300467	26.47697826356632	31.750509986078757	21.46963859905446
31	21.557507822083778	25.892131052638835	30.888128121367497	21.662233003909893
32	22.23892503204642	25.97572167624613	29.46904016944402	22.31631312226342
33	22.937485355129496	25.874901793221323	29.21312823622923	21.974484615419957
34	21.668722691623824	27.909792192159077	29.26564876202028	21.15583635419682
35	20.775471737122853	28.42198935920938	29.507748572268707	21.29479033139906
36	21.386564853229682	25.69787115270637	31.118770474103275	21.796793519960673
37	21.372896307425123	25.777355469485837	30.610593467583726	22.23915475550532
38	21.435409803678333	25.48207467850202	31.39817163099061	21.684343886829037
39	22.280390116377905	25.05272153381759	31.201643211901516	21.46524513790299
40	20.916923956940636	25.61970774581559	30.709489416640253	22.75387888060353
41	21.48276155164413	25.356444661915983	30.5379721391389	22.622821647300977
42	19.66455780531396	26.670233123366096	30.896656604779167	22.76855246654078
43	20.263676586125623	26.25383063867716	30.55818780352212	22.924304971675095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	241.0
1	406.5
2	572.0
3	1016.5
4	1461.0
5	1461.0
6	2707.5
7	3954.0
8	4442.0
9	4930.0
10	7978.5
11	11027.0
12	11027.0
13	21665.5
14	32304.0
15	51058.5
16	69813.0
17	66653.0
18	63493.0
19	63493.0
20	69830.5
21	76168.0
22	63014.0
23	49860.0
24	56206.0
25	62552.0
26	62552.0
27	70500.0
28	78448.0
29	85290.0
30	92132.0
31	100948.5
32	109765.0
33	109765.0
34	122795.0
35	135825.0
36	147550.5
37	159276.0
38	168419.5
39	177563.0
40	177563.0
41	186822.0
42	196081.0
43	216414.0
44	236747.0
45	282755.0
46	328763.0
47	328763.0
48	330411.0
49	332059.0
50	306890.0
51	281721.0
52	264458.0
53	247195.0
54	247195.0
55	221890.5
56	196586.0
57	168801.5
58	141017.0
59	127361.5
60	113706.0
61	113706.0
62	100682.5
63	87659.0
64	75413.5
65	63168.0
66	53903.0
67	44638.0
68	44638.0
69	37814.0
70	30990.0
71	26322.5
72	21655.0
73	17500.5
74	13346.0
75	13346.0
76	10435.0
77	7524.0
78	5847.0
79	4170.0
80	3326.0
81	2482.0
82	2482.0
83	1908.0
84	1334.0
85	1077.0
86	820.0
87	670.5
88	521.0
89	521.0
90	549.0
91	577.0
92	367.0
93	157.0
94	127.5
95	98.0
96	98.0
97	61.0
98	24.0
99	25.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3482448.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.13456357166511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3097682046202	31.621848570462234
2	13.628524337126205	11.757208988155107
3	5.275584587629146	6.826801163183581
4	2.566477004582563	4.428154620375329
5	1.4105943311599975	3.0422685425625673
6	0.8450698749649379	2.187103214650452
7	0.5584124036245591	1.6860812726344903
8	0.37320422481082777	1.287840108825332
9	0.2747260797123653	1.066517059513262
>10	1.4970315110232686	12.082062492480324
>50	0.1515710871861146	4.597660709887568
>100	0.09803730690920519	7.872200359651854
>500	0.005399531992658465	1.6211439491889519
>1k	0.004599601326293719	3.9811476871203824
>5k	6.666088878686549E-4	2.18819928452034
>10k+	3.3330444393432743E-4	3.7537619767882333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35286	1.0132527463439511	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34740	0.9975741202740143	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28116	0.8073630963046684	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	18601	0.5341357573752716	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13805	0.3964165437646162	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9991	0.2868958847339573	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9242	0.2653880258944283	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9135	0.26231547463163846	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8645	0.24824491277400268	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7890	0.22656476134029854	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7538	0.21645692914869083	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6686	0.19199138077582206	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6646	0.19084276348132118	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5257	0.15095702792977814	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5071	0.14561595751034906	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4701	0.1349912475362159	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4432	0.12726679623069748	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4208	0.12083453938149255	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3634	0.10435188120540492	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3609	0.10363399539634187	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3531	0.10139419167206516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.8715432362521997E-5	0.0
4	0.0	0.0	0.0	2.8715432362521997E-5	0.0
5	0.0	0.0	0.0	5.7430864725043995E-5	0.0
6	0.0	0.0	0.0	5.7430864725043995E-5	0.0
7	0.0	0.0	0.0	1.1486172945008799E-4	0.0
8	0.0	0.0	0.0	1.4357716181260998E-4	0.0
9	0.0	0.0	0.0	2.0100802653765396E-4	0.0
10	0.0	0.0	0.0	2.5843889126269797E-4	0.0
11	0.0	0.0	0.0	3.4458518835026394E-4	0.0
12	0.0	0.0	0.0	4.307314854378299E-4	0.0
13	0.0	0.0	0.0	5.455932148879179E-4	0.0
14	0.0	0.0	0.0	6.317395119754839E-4	0.0
15	0.0	0.0	0.0	8.614629708756598E-4	0.0
16	0.0	0.0	0.0	0.0015219179152136657	0.0
17	0.0	0.0	0.0	0.0022398037242767157	0.0
18	0.0	0.0	0.0	0.0028715432362521995	0.0
19	0.0	0.0	0.0	0.0035032827482276837	0.0
20	0.0	0.0	0.0	0.004651900042728563	0.0
21	0.0	0.0	0.0	0.006547118578655015	0.0
22	0.0	0.0	0.0	0.011199018621383578	0.0
23	0.0	0.0	0.0	0.018377876712014077	0.0
24	0.0	0.0	0.0	0.02908873298323478	0.0
25	0.0	0.0	0.0	0.033654486728875777	0.0
26	0.0	0.0	0.0	0.04040261333406845	0.0
27	0.0	0.0	0.0	0.07965660937363601	0.0
28	0.0	0.0	0.0	0.15302453905987973	0.0
29	0.0	0.0	0.0	0.24258797259858583	0.0
30	0.0	0.0	0.0	0.4025903617225584	0.0
31	0.0	0.0	0.0	0.622004980404589	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7990	0.0	29.93805	1
ATTGGAC	980	0.0	18.122448	3
GTATCAA	13540	0.0	17.666544	2
GTATTGG	1100	0.0	17.659092	1
TTGGACC	1640	0.0	16.92073	4
GGACCCT	1605	0.0	16.59813	6
CGAACGT	105	9.353915E-6	15.857144	4
TAACGGA	140	3.479545E-8	15.857142	28
TGGACCC	1720	0.0	15.703488	5
CGTCTGT	890	0.0	15.589889	34
TCGAACG	115	2.2122815E-5	14.478261	3
AATCGGC	115	2.2122815E-5	14.478261	25
GACCCTC	1840	0.0	14.076087	7
CGTTTAG	185	4.9094524E-9	14.000001	26
GTATGCG	80	0.006302009	13.875	33
TATACCG	160	1.7865204E-7	13.875	5
TATTGGA	1435	0.0	13.665505	2
TAACGCC	300	0.0	13.566667	4
CTAACGG	150	1.3075642E-6	13.566667	27
ACGGACC	260	1.8189894E-12	13.519231	8
>>END_MODULE