FastQCFastQC Report
Wed 25 May 2016
SRR2088137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272207
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT239081.87925392644436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT231181.817157113582931No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT152971.202398666254784No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84730.6660079688289721No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG81460.6403046045179754No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT63540.4994470239512909No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT61210.4811323943351986No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA51650.40598739041681114No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA50460.39663356670730476No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA45360.35654575080942014No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG43500.3419254885407799No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT35590.27975007211876685No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT32910.25868431788223145No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA30820.24225617372015718No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28610.22488478683107388No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24460.19226430918867762No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT24140.18974899524998684No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA22840.17953053237405547No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA20270.15932941730394504No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA20160.15846477813752008No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG19910.1564996891229179No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA19810.155713653517077No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT19310.15178347548787263No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.1509974398820318No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA19020.14950397223093412No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC19020.14950397223093412No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC17580.13818505950682552No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG17510.13763483458273693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC14920.11727651239145831No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA14710.11562583761919247No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC14490.11389655928634254No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT14120.11098822754473131No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC13650.10729386019727923No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC13510.10619341034910199No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.10375669997099528No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC12980.10202742163814536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46750.031.0641731
GTATTGG3600.024.1527771
ATTGGAC3850.023.5454543
TACGTTG656.902873E-519.9230775
GTATCAA74500.019.418792
TTGGACC6600.019.3409084
TATTGGA4650.019.0967732
ATAGGCG609.2375564E-418.55
TTTTTAC9100.018.2967051
CGTCTGT3400.017.95588334
GGACCCT6650.017.804516
CCTAGTA852.7234853E-517.4117642
GTGCTAG650.001580059617.0769231
GTACTCA650.001580059617.0769236
CGTCAAT650.001580059617.07692324
TGGACCC6950.017.035975
ACAGTCC1107.8092125E-716.8181828
CGTGTCT1107.8092125E-716.81818235
AGCACCG803.3829213E-416.18755
CCGTATA700.002592698215.8571422
Produced by FastQC (version 0.11.5)