##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088137_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1272207
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.006329158698232	31.0	31.0	33.0	30.0	34.0
2	31.18540143231408	31.0	31.0	34.0	28.0	34.0
3	31.207036276329244	31.0	31.0	34.0	28.0	34.0
4	34.98456619087931	35.0	35.0	37.0	32.0	37.0
5	34.844820850694894	35.0	35.0	37.0	32.0	37.0
6	34.95286616093136	35.0	35.0	37.0	32.0	37.0
7	34.777682405457604	35.0	35.0	37.0	32.0	37.0
8	34.892223513940735	35.0	35.0	37.0	32.0	37.0
9	36.22601982224591	38.0	35.0	39.0	32.0	39.0
10	36.031017751042086	37.0	35.0	39.0	31.0	39.0
11	36.19306999568467	38.0	35.0	39.0	32.0	39.0
12	36.00534740022653	37.0	35.0	39.0	30.0	39.0
13	36.08483996708083	38.0	35.0	39.0	31.0	39.0
14	36.854374327448284	38.0	36.0	40.0	31.0	41.0
15	36.92334502168279	38.0	36.0	40.0	31.0	41.0
16	36.7317244756553	38.0	36.0	40.0	31.0	41.0
17	36.828406855173725	38.0	36.0	40.0	31.0	41.0
18	36.83170348850462	38.0	36.0	40.0	31.0	41.0
19	36.90155611468888	39.0	36.0	40.0	31.0	41.0
20	36.828498035304	39.0	36.0	40.0	31.0	41.0
21	36.77757864875763	39.0	36.0	40.0	30.0	41.0
22	36.66453808224605	38.0	35.0	40.0	30.0	41.0
23	36.41765058673628	38.0	35.0	40.0	30.0	41.0
24	36.26098347202932	38.0	35.0	40.0	30.0	41.0
25	36.13928236521258	38.0	34.0	40.0	29.0	41.0
26	36.19215819438189	38.0	35.0	40.0	30.0	41.0
27	36.037075727456305	38.0	35.0	40.0	29.0	41.0
28	35.90070405209215	38.0	34.0	40.0	29.0	41.0
29	35.80546797808847	38.0	34.0	40.0	29.0	41.0
30	35.565126587104146	38.0	34.0	40.0	27.0	41.0
31	35.318118042111074	38.0	34.0	40.0	27.0	41.0
32	35.15369118390325	37.0	34.0	40.0	27.0	41.0
33	34.90171017766763	37.0	33.0	40.0	26.0	41.0
34	34.730535989819266	37.0	33.0	40.0	25.0	41.0
35	34.46223688440639	37.0	33.0	40.0	24.0	41.0
36	34.349380250226574	37.0	33.0	40.0	24.0	41.0
37	34.14368259253408	37.0	33.0	40.0	24.0	41.0
38	33.96902626695184	37.0	33.0	40.0	23.0	41.0
39	33.798486409837395	37.0	33.0	40.0	21.0	41.0
40	33.52376303541798	37.0	32.0	40.0	20.0	41.0
41	33.30618602161441	37.0	32.0	40.0	18.0	41.0
42	33.017340731500454	37.0	31.0	40.0	16.0	41.0
43	32.63929219065765	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	4.0
14	6.0
15	14.0
16	30.0
17	71.0
18	190.0
19	482.0
20	902.0
21	1756.0
22	3086.0
23	5170.0
24	8101.0
25	11779.0
26	16816.0
27	22638.0
28	30018.0
29	38115.0
30	46865.0
31	56973.0
32	67896.0
33	81546.0
34	97808.0
35	115999.0
36	147755.0
37	187545.0
38	214496.0
39	116141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.99342402612154	25.897829519881594	16.173311418660642	19.93543503533623
2	19.952020386619473	24.954429585751374	33.33050360515231	21.763046422476844
3	22.175557908422135	25.634271781243147	32.56789186036549	19.622278449969226
4	15.248933546191775	17.36211166893438	32.82382505362728	34.565129731246564
5	10.578860201209395	42.778730190920186	33.348818234768395	13.293591373102018
6	32.20262111433124	36.912860878772086	13.793431414856231	17.091086592040448
7	23.428891681935408	37.05442589138403	20.211962361471052	19.304720065209512
8	28.65398476820203	34.78828523974479	19.244745548483856	17.312984443569324
9	24.82779924965041	13.648093431336253	23.83338560470112	37.69072171431221
10	16.866673426572877	29.813858908180823	32.24019361629043	21.07927404895587
11	32.082043252395245	21.700556591812497	23.86915022476688	22.34824993102538
12	19.18194130357717	29.32415872574196	31.25615564133824	20.23774432934263
13	31.85228504480796	20.8844944258285	29.40189764715962	17.86132288220392
14	20.33277603408879	23.034616222045624	29.34553889422083	27.28706884964475
15	23.43580879526681	29.592747092257788	26.925492470957952	20.045951641517455
16	21.22657712149045	28.26513295399255	27.80766023139316	22.70062969312384
17	18.971047950530064	27.597474310391313	28.878948158593687	24.552529580484936
18	19.78923241264983	25.718220383946957	33.82460558698388	20.66794161641934
19	19.333567571943874	24.338256274332714	35.478581708794245	20.849594444929167
20	23.879604498324568	23.307134766590657	34.224540503235716	18.588720231849063
21	23.50238601108153	23.84525474234932	32.496598430915725	20.15576081565343
22	21.338901609565113	23.740004574727223	33.57896946015861	21.342124355549057
23	20.280268855618623	26.26074215909832	34.676668183715385	18.78232080156767
24	19.060656009595924	24.879441788954157	33.1665365777739	22.893365623676022
25	20.115358585513206	26.135133669284947	34.0685124354763	19.680995309725542
26	21.613542450245912	24.721527235740727	32.82099532544625	20.84393498856711
27	20.66754859861642	26.287781783939252	32.34984558330523	20.6948240341391
28	20.31729113265373	26.05204970574757	32.49887793417266	21.13178122742604
29	19.02984341384696	25.558497948840085	33.62055074370759	21.791107893605364
30	18.402822811067697	25.878807458220244	35.63075820208504	20.087611528627022
31	19.785538045302374	24.994989023012764	34.391808880158656	20.827664051526206
32	20.956102269520606	25.787155706579195	32.341828020125654	20.91491400377454
33	22.26720966006318	25.665477394795026	31.50304942513286	20.56426352000893
34	20.457362677614572	28.464235772952044	31.840494510720347	19.23790703871304
35	18.891658354340134	28.954171766072662	32.10035788201134	20.053811997575867
36	19.87121592633903	24.978718085971856	34.67179476295917	20.478271224729937
37	19.660322573291925	25.76781923067551	33.76101530647135	20.81084288956121
38	20.511284720175254	25.01055252800841	34.21416483323861	20.26399791857772
39	21.6017519161583	24.858061620475283	33.716368484059586	19.823817979306828
40	19.891259834287975	25.55724029187074	32.642015017996286	21.909484855844997
41	20.43951966936198	25.338014961401722	32.61481818603419	21.607647183202104
42	18.18194680582641	26.933274223455772	32.73885460463588	22.145924366081935
43	18.967274979622026	26.731498883436423	32.30716384990807	21.99406228703348
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	97.0
1	150.0
2	203.0
3	316.0
4	429.0
5	429.0
6	1027.0
7	1625.0
8	2022.0
9	2419.0
10	4072.0
11	5725.0
12	5725.0
13	12256.5
14	18788.0
15	32011.5
16	45235.0
17	40248.0
18	35261.0
19	35261.0
20	39885.0
21	44509.0
22	34157.5
23	23806.0
24	25271.5
25	26737.0
26	26737.0
27	28421.0
28	30105.0
29	31353.5
30	32602.0
31	34626.5
32	36651.0
33	36651.0
34	40110.5
35	43570.0
36	47150.0
37	50730.0
38	52979.5
39	55229.0
40	55229.0
41	58948.5
42	62668.0
43	72828.0
44	82988.0
45	107819.0
46	132650.0
47	132650.0
48	134582.5
49	136515.0
50	121974.0
51	107433.0
52	98343.0
53	89253.0
54	89253.0
55	76381.0
56	63509.0
57	52274.5
58	41040.0
59	36194.5
60	31349.0
61	31349.0
62	27318.0
63	23287.0
64	19990.0
65	16693.0
66	14106.5
67	11520.0
68	11520.0
69	9592.0
70	7664.0
71	6294.0
72	4924.0
73	4031.5
74	3139.0
75	3139.0
76	2432.0
77	1725.0
78	1299.5
79	874.0
80	705.0
81	536.0
82	536.0
83	420.5
84	305.0
85	237.5
86	170.0
87	137.0
88	104.0
89	104.0
90	96.5
91	89.0
92	56.5
93	24.0
94	19.5
95	15.0
96	15.0
97	10.5
98	6.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1272207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.665630836053836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.76401387012761	38.0200203913538
2	11.541453867474512	11.002613587167719
3	3.6389027107244205	5.203517797731174
4	1.5530562031037574	2.9610961457914855
5	0.8130808130260998	1.9378004936790292
6	0.503367611324106	1.4396000841720642
7	0.33731849881799175	1.125494932718118
8	0.24560285038176055	0.9365451838863651
9	0.1937386664868216	0.8311208179887175
>10	1.2307288124436802	11.232167864293778
>50	0.10021414174324016	3.292836264398253
>100	0.06265225671293309	5.626174425676968
>500	0.00859608621795314	3.008657918850955
>1k	0.005785827262083844	5.374500152659191
>5k	9.91856102071516E-4	3.0970667443741133
>10k+	4.95928051035758E-4	4.91078719525831
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23908	1.87925392644436	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23118	1.817157113582931	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15297	1.202398666254784	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8473	0.6660079688289721	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8146	0.6403046045179754	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6354	0.4994470239512909	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6121	0.4811323943351986	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5165	0.40598739041681114	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5046	0.39663356670730476	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4536	0.35654575080942014	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4350	0.3419254885407799	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3559	0.27975007211876685	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3291	0.25868431788223145	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3082	0.24225617372015718	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2861	0.22488478683107388	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2446	0.19226430918867762	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2414	0.18974899524998684	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2284	0.17953053237405547	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2027	0.15932941730394504	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2016	0.15846477813752008	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1991	0.1564996891229179	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1981	0.155713653517077	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1931	0.15178347548787263	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1921	0.1509974398820318	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1902	0.14950397223093412	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1902	0.14950397223093412	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	1758	0.13818505950682552	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1751	0.13763483458273693	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1492	0.11727651239145831	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1471	0.11562583761919247	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1449	0.11389655928634254	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT	1412	0.11098822754473131	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1365	0.10729386019727923	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1351	0.10619341034910199	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1320	0.10375669997099528	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1298	0.10202742163814536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	7.860356058408734E-5	0.0	0.0	7.860356058408734E-5	0.0
10	7.860356058408734E-5	0.0	0.0	3.930178029204367E-4	0.0
11	7.860356058408734E-5	0.0	0.0	7.074320452567861E-4	0.0
12	7.860356058408734E-5	0.0	0.0	9.432427270090481E-4	0.0
13	7.860356058408734E-5	0.0	0.0	0.0011004498481772228	0.0
14	1.5720712116817467E-4	0.0	0.0	0.0015720712116817468	0.0
15	1.5720712116817467E-4	0.0	0.0	0.0018078818934340087	0.0
16	1.5720712116817467E-4	0.0	0.0	0.0025153139386907947	0.0
17	1.5720712116817467E-4	0.0	0.0	0.003930178029204367	0.0
18	1.5720712116817467E-4	0.0	0.0	0.004716213635045241	0.0
19	1.5720712116817467E-4	0.0	0.0	0.006052474164974725	0.0
20	1.5720712116817467E-4	0.0	0.0	0.007545941816072385	0.0
21	1.5720712116817467E-4	0.0	0.0	0.009982652194179093	0.0
22	1.5720712116817467E-4	0.0	0.0	0.01855044029784461	0.0
23	1.5720712116817467E-4	0.0	0.0	0.03018376726428954	0.0
24	1.5720712116817467E-4	0.0	0.0	0.044411011730009345	0.0
25	1.5720712116817467E-4	0.0	0.0	0.0496774502891432	0.0
26	1.5720712116817467E-4	0.0	0.0	0.057223392105215584	0.0
27	1.5720712116817467E-4	0.0	0.0	0.09888327921478188	0.0
28	1.5720712116817467E-4	0.0	0.0	0.17127715851272632	0.0
29	1.5720712116817467E-4	0.0	0.0	0.256483418185877	0.0
30	1.5720712116817467E-4	0.0	0.0	0.4448961529059343	0.0
31	1.5720712116817467E-4	0.0	0.0	0.6627852228450244	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4675	0.0	31.064173	1
GTATTGG	360	0.0	24.152777	1
ATTGGAC	385	0.0	23.545454	3
TACGTTG	65	6.902873E-5	19.923077	5
GTATCAA	7450	0.0	19.41879	2
TTGGACC	660	0.0	19.340908	4
TATTGGA	465	0.0	19.096773	2
ATAGGCG	60	9.2375564E-4	18.5	5
TTTTTAC	910	0.0	18.296705	1
CGTCTGT	340	0.0	17.955883	34
GGACCCT	665	0.0	17.80451	6
CCTAGTA	85	2.7234853E-5	17.411764	2
GTGCTAG	65	0.0015800596	17.076923	1
GTACTCA	65	0.0015800596	17.076923	6
CGTCAAT	65	0.0015800596	17.076923	24
TGGACCC	695	0.0	17.03597	5
ACAGTCC	110	7.8092125E-7	16.818182	8
CGTGTCT	110	7.8092125E-7	16.818182	35
AGCACCG	80	3.3829213E-4	16.1875	5
CCGTATA	70	0.0025926982	15.857142	2
>>END_MODULE