FastQCFastQC Report
Wed 25 May 2016
SRR2088136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679982
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT113031.6622498830851404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112511.6546026218341072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76921.131206414287437No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG49740.7314899512045907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42550.6257518581374213No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT33270.48927765734975337No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT31120.4576591733310587No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29340.43148200981790696No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA26110.383980752431682No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA25010.36780385363141965No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG23890.35133282939842525No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT18650.27427196602263No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT18040.2653011403243027No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA16720.24588886176398786No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13630.20044648240688723No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA12820.1885344023812395No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT12180.17912238853381413No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12150.17868120038471608No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.17456344432646745No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA11580.17029862555185285No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG10770.1583865455262051No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT10600.15588647934798275No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA10030.14750390451511952No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA10010.14720977908238747No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG9910.14573915191872724No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.14279789759140685No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC9310.13691538893676597No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC8900.1308858175657591No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC8200.1205914274201376No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7960.1170619222273531No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC7420.10912053554358792No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA7140.10500277948533931No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA7100.10441452861987524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.030.1803911
CCGATAA250.00549478329.69
GCGATAT250.00549478329.630
TGCGTGC502.700529E-422.28
GTATTGG2400.020.81251
ATCGCAG450.00382422620.55555511
TCACGTT450.00382422620.55555524
ATTGGAC2550.019.5882363
GTATCAA40250.019.0745352
CAGGTCG500.00703199818.537
TACCCCA701.2182683E-418.55
ACTGCGC609.2315266E-418.58
ACGATCT609.2315266E-418.532
GCCTATA650.001579036917.0769231
ATTATAG700.00259103415.8571421
CGATCTG700.00259103415.85714233
TAGTCAA700.00259103415.85714226
ACAGTGT1302.58422E-715.6538468
TATTGGA3450.015.5507242
TTTTTAC4700.015.3510651
Produced by FastQC (version 0.11.5)