FastQCFastQC Report
Wed 25 May 2016
SRR2088135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558073
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT242981.559490473167817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT227181.4580831578494717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT189781.2180430570326295No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG131150.8417448989874031No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90340.5798187889784369No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT69760.44773255168403536No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA66830.4289272710585447No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT63260.40601435234420985No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA61930.39747816694083016No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA56740.36416778931410787No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG51230.3288035926429635No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT48490.3112177670750985No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT46060.2956215787065176No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37160.2384997365335257No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA36510.23432791659954316No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA33130.2126344529428339No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT31230.20043990236657716No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.17739861996196585No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA26410.16950425300996808No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC24510.15730970243371137No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG23130.14845260780464073No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT22700.14569278846369843No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG22600.14505097001231648No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.14338224203872346No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC22010.1412642411491631No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21960.14094333192347214No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21640.13888951287904996No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC19370.12432023403268011No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC17900.11488550279736573No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG17800.1142436843459838No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA17690.11353768404946366No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC16790.10776131798702629No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA15880.10192077007945072No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC15770.10121476978293058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCACG250.005496937329.59999817
GGTATCA55100.028.539021
GTATTGG7400.020.751
CATACCG500.007035979518.49999817
CGTCTGT4450.018.29213534
ATTGGAC8200.018.274393
TTGGACC11550.017.9393944
AGTACCG752.0678605E-417.2666665
GGACCCT11600.017.2241386
TTTTTAC11100.017.01
CGGTATA1201.04188075E-716.95833426
GTATCAA93450.016.8073842
TATTGGA8950.016.5363142
GGACTTT3600.016.4444453
AACGTGA803.3835307E-416.18756
ACCGTGT803.3835307E-416.18758
TGGACCC12200.016.073775
TAAACGT700.00259304815.8571434
GGCGTCG957.0622504E-515.5789488
GGTCGGT1455.3489202E-815.31034523
Produced by FastQC (version 0.11.5)