##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088135_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1558073
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.590750240842375	31.0	31.0	34.0	30.0	34.0
2	31.759810355483985	31.0	31.0	34.0	30.0	34.0
3	31.852372128905387	31.0	31.0	34.0	30.0	34.0
4	35.5590880529988	37.0	35.0	37.0	33.0	37.0
5	35.47701808580214	37.0	35.0	37.0	33.0	37.0
6	35.51325451374871	37.0	35.0	37.0	33.0	37.0
7	35.35828873229945	37.0	35.0	37.0	33.0	37.0
8	35.435801146672844	37.0	35.0	37.0	33.0	37.0
9	36.907200111933136	39.0	37.0	39.0	33.0	39.0
10	36.798933682824874	39.0	37.0	39.0	32.0	39.0
11	36.876844024638125	39.0	37.0	39.0	33.0	39.0
12	36.75186400123743	39.0	35.0	39.0	32.0	39.0
13	36.75511994624129	39.0	35.0	39.0	32.0	39.0
14	37.71683419197945	39.0	37.0	41.0	32.0	41.0
15	37.70950077435396	39.0	37.0	41.0	32.0	41.0
16	37.59346577471017	39.0	36.0	41.0	32.0	41.0
17	37.66601629063593	39.0	37.0	41.0	32.0	41.0
18	37.6643520553915	39.0	37.0	41.0	32.0	41.0
19	37.724614957065555	39.0	37.0	41.0	32.0	41.0
20	37.65064281326998	39.0	37.0	41.0	32.0	41.0
21	37.57326967350053	39.0	36.0	41.0	32.0	41.0
22	37.48629685515377	39.0	36.0	41.0	32.0	41.0
23	37.25156202565605	39.0	36.0	41.0	31.0	41.0
24	37.09485948347735	39.0	36.0	41.0	31.0	41.0
25	36.99861174668966	39.0	36.0	41.0	31.0	41.0
26	37.12238001685415	39.0	36.0	41.0	31.0	41.0
27	37.010169613362145	39.0	36.0	41.0	31.0	41.0
28	36.92150688703289	39.0	35.0	41.0	31.0	41.0
29	36.82982761398215	39.0	35.0	40.0	30.0	41.0
30	36.62066026431368	39.0	35.0	40.0	30.0	41.0
31	36.40366080408299	38.0	35.0	40.0	30.0	41.0
32	36.24439612264637	38.0	35.0	40.0	30.0	41.0
33	36.015179006375185	38.0	35.0	40.0	30.0	41.0
34	35.87046434923139	38.0	35.0	40.0	29.0	41.0
35	35.64048218536615	38.0	35.0	40.0	27.0	41.0
36	35.53422272255536	38.0	34.0	40.0	27.0	41.0
37	35.36175583557382	38.0	34.0	40.0	26.0	41.0
38	35.20953703709647	38.0	34.0	40.0	25.0	41.0
39	35.07182847016796	38.0	34.0	40.0	25.0	41.0
40	34.817975794458924	38.0	33.0	40.0	24.0	41.0
41	34.61930731101816	38.0	33.0	40.0	23.0	41.0
42	34.357738052068164	38.0	33.0	40.0	21.0	41.0
43	34.01482279713466	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	5.0
14	6.0
15	7.0
16	15.0
17	45.0
18	105.0
19	236.0
20	543.0
21	1065.0
22	2027.0
23	3557.0
24	5808.0
25	8841.0
26	13290.0
27	18914.0
28	25385.0
29	33543.0
30	42510.0
31	52887.0
32	65501.0
33	81748.0
34	101414.0
35	124599.0
36	161746.0
37	224590.0
38	286053.0
39	303631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53937139017235	23.403717284106715	14.359853485683919	21.697057840037022
2	17.828368760642153	23.035441856703763	36.451886400701376	22.684302981952705
3	19.754851024310156	26.090626048972034	32.82779433312816	21.326728593589646
4	12.815253200588161	15.528476521960139	35.21503806304326	36.44123221440844
5	10.485773131297442	41.9377654320433	34.147116341788866	13.429345094870396
6	31.264838040322886	36.63948993404032	15.139983813338656	16.95568821229814
7	24.654878173230653	36.025205494222675	20.39269020129352	18.927226131253157
8	28.788766636736536	33.634560126515254	19.46526253904663	18.111410697701583
9	25.423840859831344	13.643070639180577	23.6223206486474	37.31076785234068
10	16.464440369610408	28.59320455460046	32.662975354813284	22.279379720975847
11	31.282680593271305	21.826448439835616	24.974824671244544	21.916046295648535
12	20.09854480502518	28.19476365998256	31.484211587005227	20.222479947987033
13	31.701659678333428	21.009991187832664	28.7280506112358	18.560298522598107
14	20.741005074858496	22.780383204124583	28.525685253515082	27.952926467501843
15	23.083257331331716	29.176168253990664	27.0371799010701	20.703394513607513
16	21.56811651315439	28.88856940592642	26.79508598120884	22.748228099710346
17	19.017209078136904	27.30597346850886	27.838939510536413	25.837877942817826
18	20.243082320276393	26.623720454689863	32.70989228360931	20.42330494142444
19	19.412633426033313	24.597563785522244	34.04461793510317	21.945184853341274
20	24.917895374606967	23.551848982685662	32.50297001488377	19.027285627823602
21	23.836944738789516	24.636907256591954	31.12299616256748	20.403151842051045
22	21.875932642437164	24.051568828931636	32.395016151361325	21.67748237726987
23	20.686963961252136	26.45864474899443	33.63218539824514	19.222205891508292
24	19.18080860139416	25.40586994319265	31.665846208746316	23.747475246666877
25	20.568227547746478	26.630973003190476	32.88972981368652	19.911069635376517
26	22.45190052070731	25.202926948865684	31.199565103817346	21.145607426609665
27	20.631639210743014	27.112593569107478	30.839633316282356	21.41613390386715
28	20.460081138688622	26.28952558705529	31.094242695945567	22.156150578310516
29	19.24640244712539	26.230735016908707	32.46927454618621	22.053587989779682
30	18.58609962434366	26.42026400560179	34.5983788949555	20.39525747509905
31	20.139236094842797	25.930363981661962	33.215452677762855	20.714947245732386
32	21.588911430979167	26.077404588873566	30.926920625670302	21.406763354476972
33	22.807724670153455	25.960529448876912	30.364110025653485	20.86763585531615
34	20.429594762247984	29.293813576128976	30.515258270953925	19.761333390669115
35	18.95411832436606	30.197814864900423	30.847527683234354	20.000539127499163
36	20.0979029865738	25.78768773991976	33.45677641548246	20.657632858023984
37	20.190517389108212	26.37649198721754	32.37807214424485	21.054918479429396
38	20.537484443925287	25.711888980811555	33.304922169885494	20.44570440537767
39	21.752639317926693	25.31068826685271	33.00962150040467	19.92705091481593
40	19.738933926715884	26.07708367964787	31.897093396779226	22.286888996857016
41	20.869304583289743	25.596040750337117	31.878416479844013	21.656238186529126
42	18.12642924946392	27.525475378881474	32.32197721159407	22.026118160060538
43	19.031008174841617	27.222986342745177	31.780603347853404	21.965402134559806
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	165.0
2	245.0
3	520.5
4	796.0
5	796.0
6	1521.5
7	2247.0
8	2620.0
9	2993.0
10	4883.5
11	6774.0
12	6774.0
13	13439.0
14	20104.0
15	33084.0
16	46064.0
17	42967.5
18	39871.0
19	39871.0
20	43115.0
21	46359.0
22	36623.5
23	26888.0
24	30005.0
25	33122.0
26	33122.0
27	35466.0
28	37810.0
29	39484.5
30	41159.0
31	43712.5
32	46266.0
33	46266.0
34	50602.5
35	54939.0
36	58844.0
37	62749.0
38	65623.0
39	68497.0
40	68497.0
41	72056.5
42	75616.0
43	87787.5
44	99959.0
45	131801.0
46	163643.0
47	163643.0
48	165806.5
49	167970.0
50	151080.5
51	134191.0
52	125082.5
53	115974.0
54	115974.0
55	99821.5
56	83669.0
57	68200.0
58	52731.0
59	45692.0
60	38653.0
61	38653.0
62	33715.0
63	28777.0
64	24825.0
65	20873.0
66	17522.0
67	14171.0
68	14171.0
69	11866.0
70	9561.0
71	7913.0
72	6265.0
73	5149.0
74	4033.0
75	4033.0
76	3138.5
77	2244.0
78	1678.0
79	1112.0
80	883.0
81	654.0
82	654.0
83	534.0
84	414.0
85	314.5
86	215.0
87	169.0
88	123.0
89	123.0
90	142.0
91	161.0
92	107.0
93	53.0
94	41.5
95	30.0
96	30.0
97	18.0
98	6.0
99	6.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1558073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.57850577586831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7488804992784	34.65928918404377
2	12.374415413091292	11.032658979309678
3	4.0638840046741365	5.434856297244744
4	1.7839050372500518	3.1809528402660803
5	0.9736457915561717	2.1701837271268336
6	0.5758975777136898	1.5403592096650973
7	0.3934409979852103	1.2277308260802962
8	0.2698905867851695	0.9625055265484131
9	0.20827495141766458	0.8356127512266948
>10	1.376469528607106	11.95881039138257
>50	0.13743743040430448	4.2084757191744915
>100	0.07809467180689074	6.560971229014079
>500	0.007086348275821195	2.3088335413644305
>1k	0.007086348275821195	5.852509113591796
>5k	0.0010123354679744566	2.96616095844068
>10k+	5.784774102711181E-4	5.100089705520306
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24298	1.559490473167817	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22718	1.4580831578494717	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18978	1.2180430570326295	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	13115	0.8417448989874031	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9034	0.5798187889784369	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6976	0.44773255168403536	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6683	0.4289272710585447	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6326	0.40601435234420985	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6193	0.39747816694083016	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5674	0.36416778931410787	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5123	0.3288035926429635	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4849	0.3112177670750985	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4606	0.2956215787065176	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3716	0.2384997365335257	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3651	0.23432791659954316	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3313	0.2126344529428339	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3123	0.20043990236657716	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2764	0.17739861996196585	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2641	0.16950425300996808	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2451	0.15730970243371137	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2313	0.14845260780464073	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2270	0.14569278846369843	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2260	0.14505097001231648	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2234	0.14338224203872346	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2201	0.1412642411491631	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2196	0.14094333192347214	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2164	0.13888951287904996	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1937	0.12432023403268011	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1790	0.11488550279736573	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1780	0.1142436843459838	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1769	0.11353768404946366	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	1679	0.10776131798702629	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1588	0.10192077007945072	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1577	0.10121476978293058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.2836369027638628E-4	0.0
10	0.0	0.0	0.0	1.9254553541457942E-4	0.0
11	0.0	0.0	0.0	3.8509107082915884E-4	0.0
12	0.0	0.0	0.0	8.343639867965108E-4	0.0
13	0.0	0.0	0.0	0.0010269095222110902	0.0
14	6.418184513819314E-5	0.0	0.0	0.001283636902763863	0.0
15	6.418184513819314E-5	0.0	0.0	0.0020538190444221805	0.0
16	6.418184513819314E-5	0.0	0.0	0.003594183327738816	0.0
17	6.418184513819314E-5	0.0	0.0	0.006225638978404734	0.0
18	6.418184513819314E-5	0.0	0.0	0.006931639274924859	0.0
19	6.418184513819314E-5	0.0	0.0	0.008022730642274142	0.0
20	1.2836369027638628E-4	0.0	0.0	0.010461640757525481	0.0
21	1.2836369027638628E-4	0.0	0.0	0.014440915156093456	0.0
22	1.2836369027638628E-4	0.0	0.0	0.023618919010855076	0.0
23	1.2836369027638628E-4	0.0	0.0	0.03844492523777769	0.0
24	1.2836369027638628E-4	0.0	0.0	0.05923984306255227	0.0
25	1.2836369027638628E-4	0.0	0.0	0.06572220942150978	0.0
26	1.9254553541457942E-4	0.0	0.0	0.07380912190892211	0.0
27	1.9254553541457942E-4	0.0	0.0	0.11892895904107188	0.0
28	1.9254553541457942E-4	0.0	0.0	0.19690990088397656	0.0
29	1.9254553541457942E-4	0.0	0.0	0.29324685043640447	0.0
30	1.9254553541457942E-4	0.0	0.0	0.48001601978854647	0.0
31	1.9254553541457942E-4	0.0	0.0	0.7069630241971975	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCACG	25	0.0054969373	29.599998	17
GGTATCA	5510	0.0	28.53902	1
GTATTGG	740	0.0	20.75	1
CATACCG	50	0.0070359795	18.499998	17
CGTCTGT	445	0.0	18.292135	34
ATTGGAC	820	0.0	18.27439	3
TTGGACC	1155	0.0	17.939394	4
AGTACCG	75	2.0678605E-4	17.266666	5
GGACCCT	1160	0.0	17.224138	6
TTTTTAC	1110	0.0	17.0	1
CGGTATA	120	1.04188075E-7	16.958334	26
GTATCAA	9345	0.0	16.807384	2
TATTGGA	895	0.0	16.536314	2
GGACTTT	360	0.0	16.444445	3
AACGTGA	80	3.3835307E-4	16.1875	6
ACCGTGT	80	3.3835307E-4	16.1875	8
TGGACCC	1220	0.0	16.07377	5
TAAACGT	70	0.002593048	15.857143	4
GGCGTCG	95	7.0622504E-5	15.578948	8
GGTCGGT	145	5.3489202E-8	15.310345	23
>>END_MODULE