FastQCFastQC Report
Wed 25 May 2016
SRR2088134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724317
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT149650.867879861997533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT144390.8373750302293604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121750.7060766668773781No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG95140.5517546947574026No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62470.3622883727296083No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT47390.2748334557972809No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA45960.26654031712266363No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT41210.23899317816851542No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA38990.22611851533099772No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG38250.22182696105182514No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA34750.20152906919087385No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT31320.18163713516714156No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT29720.1723580988878495No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26550.15397400825950217No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA26320.15264014679435392No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21110.12242528490990925No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT18760.10879670037469907No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC17840.10346125451410618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43200.024.6238421
CGCGCTT450.003826572820.55555517
GCGCTTA555.1445275E-420.18181818
GCGTAAG555.1445275E-420.1818189
GCCGTCA701.2196459E-418.513
ATTGGAC5300.017.8018863
ATAACGC1054.800695E-717.6190473
GTATCAA65350.016.2211192
TTGGACC7650.016.2026144
TATACTG3300.015.696975
GGACCCT7950.015.5911946
TATACCG957.062949E-515.5789485
TGGACCC7950.015.358495
TATTGGA6350.015.1496072
ACCGACC1601.0973963E-815.0312518
GTAATAC2350.014.9574473
CACGGGT750.00410551414.818
GACCGTG1001.0938678E-414.7999997
AAGACCG1152.2110004E-514.4782615
CCCGTAT908.2769745E-414.3888881
Produced by FastQC (version 0.11.5)