##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088134_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1724317
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52411708519953	31.0	31.0	34.0	30.0	34.0
2	31.701521819943782	31.0	31.0	34.0	30.0	34.0
3	31.79984422817846	31.0	31.0	34.0	30.0	34.0
4	35.5297755575106	37.0	35.0	37.0	33.0	37.0
5	35.40176951221846	37.0	35.0	37.0	33.0	37.0
6	35.42280450752385	37.0	35.0	37.0	33.0	37.0
7	35.30017972333393	37.0	35.0	37.0	32.0	37.0
8	35.31683907309387	37.0	35.0	37.0	32.0	37.0
9	36.84005609177431	39.0	37.0	39.0	33.0	39.0
10	36.68237278876216	39.0	35.0	39.0	32.0	39.0
11	36.77400327202017	39.0	35.0	39.0	32.0	39.0
12	36.658293689617395	39.0	35.0	39.0	32.0	39.0
13	36.678671033226486	39.0	35.0	39.0	32.0	39.0
14	37.69000595598141	39.0	37.0	41.0	32.0	41.0
15	37.6954144742527	39.0	37.0	41.0	32.0	41.0
16	37.57700701205173	39.0	36.0	41.0	32.0	41.0
17	37.585711328021475	39.0	36.0	41.0	32.0	41.0
18	37.5652249557361	39.0	36.0	41.0	32.0	41.0
19	37.5907324465281	39.0	37.0	41.0	32.0	41.0
20	37.513101709256475	39.0	36.0	41.0	32.0	41.0
21	37.43081927511009	39.0	36.0	41.0	32.0	41.0
22	37.35815978152509	39.0	36.0	41.0	32.0	41.0
23	37.158628604833105	39.0	36.0	40.0	31.0	41.0
24	37.034707075323155	39.0	36.0	40.0	31.0	41.0
25	36.94024126654206	39.0	36.0	40.0	31.0	41.0
26	37.04477482968619	39.0	36.0	40.0	31.0	41.0
27	36.951882397494195	39.0	36.0	40.0	31.0	41.0
28	36.872987971469286	39.0	36.0	40.0	30.0	41.0
29	36.779468044448905	39.0	35.0	40.0	30.0	41.0
30	36.62438635123356	39.0	35.0	40.0	30.0	41.0
31	36.49685991612911	39.0	35.0	40.0	30.0	41.0
32	36.36073761379143	38.0	35.0	40.0	30.0	41.0
33	36.19957641199385	38.0	35.0	40.0	30.0	41.0
34	36.07858473818909	38.0	35.0	40.0	29.0	41.0
35	35.90416147378933	38.0	35.0	40.0	29.0	41.0
36	35.80848533071355	38.0	35.0	40.0	28.0	41.0
37	35.6782859532209	38.0	35.0	40.0	27.0	41.0
38	35.590209920797626	38.0	34.0	40.0	27.0	41.0
39	35.52608424089074	38.0	34.0	40.0	26.0	41.0
40	35.360241765290255	38.0	34.0	40.0	25.0	41.0
41	35.24443823264516	38.0	34.0	40.0	25.0	41.0
42	35.08755350669279	38.0	34.0	40.0	24.0	41.0
43	34.775759329636024	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	3.0
14	5.0
15	13.0
16	23.0
17	52.0
18	108.0
19	238.0
20	524.0
21	1038.0
22	1948.0
23	3565.0
24	5887.0
25	9201.0
26	13611.0
27	19609.0
28	26718.0
29	35808.0
30	46656.0
31	59043.0
32	73481.0
33	90811.0
34	111845.0
35	138267.0
36	175559.0
37	236243.0
38	324001.0
39	350058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.41101491199124	22.672919190612863	13.494096503137184	23.421969394258713
2	17.628835069189712	22.712935034567312	36.29837205107877	23.3598578451642
3	19.379731221115374	26.072642095391974	31.84356472736742	22.704061956125237
4	13.202386800106941	16.089732920338893	36.312464587427954	34.395415692126214
5	11.42881500327376	40.404461592618986	35.1754346793542	12.991288724753048
6	31.47240327619573	37.410812513012395	15.798603157076105	15.31818105371576
7	25.508128725750545	34.29862374493785	22.01654336180644	18.17670416750516
8	27.26714403442059	34.88320303053325	20.147629467203537	17.70202346784263
9	25.57302398572884	14.09050655998868	22.485076699933945	37.85139275434853
10	15.625607124444056	28.166688607721202	34.435605518010895	21.772098749823844
11	32.232182365539515	22.29798813095272	24.418537890654676	21.051291612853092
12	19.604979826795187	27.25589320293194	32.28327505905237	20.855851911220498
13	30.30365066284216	21.191637036577383	27.905715712366113	20.598996588214348
14	20.671663041076556	22.30581731781337	28.102373287510357	28.920146353599712
15	23.115587215111837	29.23899723774689	25.932296671667682	21.713118875473594
16	22.04339457303964	28.878912636133613	26.358726382677894	22.718966408148848
17	20.027291965456467	27.665446666709197	27.83931260899243	24.467948758841906
18	20.95183194273443	27.136715580719788	30.775025705830195	21.136426770715595
19	20.605201943726126	25.860790098340384	31.57273285596558	21.96127510196791
20	23.989440456714163	25.044292899739435	30.77258995880688	20.193676684739522
21	23.197938662090557	25.86606755022423	29.681897238152843	21.254096549532367
22	22.099996694343325	25.3225480001647	30.72816657261977	21.849288732872203
23	21.34827876776718	26.80191635296758	31.230974351003905	20.618830528261334
24	20.532187527003444	26.215597248069816	29.942638157600953	23.309577067325787
25	21.542674577818346	26.8684934382715	30.587589172988494	21.001242810921656
26	22.35685201734948	26.193965494743715	29.716635630223443	21.73254685768336
27	21.42564273274578	27.42111804268009	29.355275161121764	21.79796406345237
28	21.441533082374065	26.581945199171614	29.665832906594318	22.31068881186
29	20.522618520840425	26.64121504340559	30.762151042992674	22.07401539276131
30	20.29725392720712	26.721826671081946	31.858585167344522	21.12233423436642
31	21.155390801111395	26.54030552386829	30.908411852345015	21.3958918226753
32	21.817276057708646	26.51014865596059	29.806004348388377	21.866570937942384
33	22.54388259235396	26.432784690981993	29.419996439169825	21.60333627749422
34	21.33528811697617	28.223522704931863	29.519862067125707	20.921327110966256
35	20.467292267025147	28.80671013508537	29.775093558782984	20.9509040391065
36	21.24632535664846	26.25822282097781	31.056122511115998	21.439329311257733
37	21.33714392423203	26.31354907479309	30.58271767894187	21.766589322033013
38	21.302057568300956	26.075599788205995	31.273831899818884	21.34851074367416
39	21.966842523735487	25.75500908475646	31.24715467051592	21.03099372099214
40	20.83972958568523	26.11944323462565	30.549835094127126	22.490992085561995
41	21.49714930607307	25.81190117594387	30.474210948450892	22.21673856953217
42	19.76092563026404	27.14495072541766	30.74533278973646	22.348790854581843
43	20.475759387630003	26.73011980975656	30.33340157291264	22.460719229700803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	146.5
2	196.0
3	398.0
4	600.0
5	600.0
6	1199.5
7	1799.0
8	2043.0
9	2287.0
10	3639.0
11	4991.0
12	4991.0
13	9802.0
14	14613.0
15	22614.0
16	30615.0
17	30104.0
18	29593.0
19	29593.0
20	33329.0
21	37065.0
22	32650.5
23	28236.0
24	32208.5
25	36181.0
26	36181.0
27	40397.0
28	44613.0
29	48747.5
30	52882.0
31	57836.5
32	62791.0
33	62791.0
34	68649.0
35	74507.0
36	80970.5
37	87434.0
38	90362.0
39	93290.0
40	93290.0
41	96863.5
42	100437.0
43	108689.5
44	116942.0
45	137580.5
46	158219.0
47	158219.0
48	160679.0
49	163139.0
50	149504.0
51	135869.0
52	126420.5
53	116972.0
54	116972.0
55	104364.0
56	91756.0
57	79042.5
58	66329.0
59	58856.5
60	51384.0
61	51384.0
62	45403.0
63	39422.0
64	33782.5
65	28143.0
66	23970.0
67	19797.0
68	19797.0
69	16575.0
70	13353.0
71	11039.0
72	8725.0
73	6996.0
74	5267.0
75	5267.0
76	3996.5
77	2726.0
78	2251.0
79	1776.0
80	1394.0
81	1012.0
82	1012.0
83	763.5
84	515.0
85	410.0
86	305.0
87	251.5
88	198.0
89	198.0
90	173.0
91	148.0
92	96.0
93	44.0
94	38.5
95	33.0
96	33.0
97	21.5
98	10.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1724317.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.98654867914297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76504027148529	46.02520899003938
2	11.75018366717448	13.392048270766185
3	3.480489430408394	5.950232410596316
4	1.374930195523622	3.1341010607052175
5	0.6913470337166809	1.9698740695538368
6	0.38872786274431675	1.3291355563930927
7	0.25422148408075784	1.0141043484496455
8	0.18440317336805756	0.8406800332581803
9	0.1336447699603108	0.6854358770150504
>10	0.8517147319314393	9.289534486197116
>50	0.07586940063577614	2.9791254612027087
>100	0.04086767720743389	4.3534997530901265
>500	0.004993509362014158	1.947281102882322
>1k	0.0030572506298045866	3.759772326554664
>5k	2.0381670865363914E-4	0.915305164251835
>10k+	3.0572506298045865E-4	2.4146610890442894
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14965	0.867879861997533	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14439	0.8373750302293604	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12175	0.7060766668773781	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9514	0.5517546947574026	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6247	0.3622883727296083	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4739	0.2748334557972809	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4596	0.26654031712266363	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4121	0.23899317816851542	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3899	0.22611851533099772	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3825	0.22182696105182514	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3475	0.20152906919087385	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3132	0.18163713516714156	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2972	0.1723580988878495	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2655	0.15397400825950217	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2632	0.15264014679435392	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2111	0.12242528490990925	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1876	0.10879670037469907	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1784	0.10346125451410618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1598795349115041E-4	0.0
2	0.0	0.0	0.0	2.3197590698230083E-4	0.0
3	0.0	0.0	0.0	2.3197590698230083E-4	0.0
4	0.0	0.0	0.0	2.8996988372787604E-4	0.0
5	0.0	0.0	0.0	2.8996988372787604E-4	0.0
6	0.0	0.0	0.0	2.8996988372787604E-4	0.0
7	0.0	0.0	0.0	2.8996988372787604E-4	0.0
8	0.0	0.0	0.0	2.8996988372787604E-4	0.0
9	0.0	0.0	0.0	3.4796386047345124E-4	0.0
10	0.0	0.0	0.0	4.6395181396460166E-4	0.0
11	0.0	0.0	0.0	7.539216976924776E-4	0.0
12	0.0	0.0	0.0	0.001101885558165929	0.0
13	0.0	0.0	0.0	0.0013338614651482296	0.0
14	0.0	0.0	0.0	0.0016238313488761058	0.0
15	0.0	0.0	0.0	0.001971795209349557	0.0
16	5.799397674557521E-5	0.0	0.0	0.0031896687210066364	0.0
17	5.799397674557521E-5	0.0	0.0	0.004581524162900441	0.0
18	5.799397674557521E-5	0.0	0.0	0.005393439837338494	0.0
19	5.799397674557521E-5	0.0	0.0	0.005915385628048671	0.0
20	5.799397674557521E-5	0.0	0.0	0.007307241069942476	0.0
21	5.799397674557521E-5	0.0	0.0	0.010438915814203536	0.0
22	5.799397674557521E-5	0.0	0.0	0.016876247232962386	0.0
23	5.799397674557521E-5	0.0	0.0	0.026561241349473443	0.0
24	5.799397674557521E-5	0.0	0.0	0.03908794032651769	0.0
25	5.799397674557521E-5	0.0	0.0	0.044655362094092904	0.0
26	5.799397674557521E-5	0.0	0.0	0.04964284409421237	0.0
27	5.799397674557521E-5	0.0	0.0	0.07892980235072786	0.0
28	5.799397674557521E-5	0.0	0.0	0.1349519838869535	0.0
29	5.799397674557521E-5	0.0	0.0	0.20216700293507517	0.0
30	5.799397674557521E-5	0.0	0.0	0.3199527697053384	0.0
31	5.799397674557521E-5	0.0	0.0	0.4832058142441326	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4320	0.0	24.623842	1
CGCGCTT	45	0.0038265728	20.555555	17
GCGCTTA	55	5.1445275E-4	20.181818	18
GCGTAAG	55	5.1445275E-4	20.181818	9
GCCGTCA	70	1.2196459E-4	18.5	13
ATTGGAC	530	0.0	17.801886	3
ATAACGC	105	4.800695E-7	17.619047	3
GTATCAA	6535	0.0	16.221119	2
TTGGACC	765	0.0	16.202614	4
TATACTG	330	0.0	15.69697	5
GGACCCT	795	0.0	15.591194	6
TATACCG	95	7.062949E-5	15.578948	5
TGGACCC	795	0.0	15.35849	5
TATTGGA	635	0.0	15.149607	2
ACCGACC	160	1.0973963E-8	15.031251	8
GTAATAC	235	0.0	14.957447	3
CACGGGT	75	0.004105514	14.8	18
GACCGTG	100	1.0938678E-4	14.799999	7
AAGACCG	115	2.2110004E-5	14.478261	5
CCCGTAT	90	8.2769745E-4	14.388888	1
>>END_MODULE