FastQCFastQC Report
Wed 25 May 2016
SRR2088133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260668
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT163371.2959002687464107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT160501.2731345604076567No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130531.035403452772657No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG101880.8081429845129724No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65050.5159962813365613No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT51560.4089895198418616No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT47060.3732941583351049No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA45800.363299457113213No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA40990.32514508181376856No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA40830.32387591340463945No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG37800.2998410366567566No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT35180.2790584039572671No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT34280.27191933165591575No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27090.21488607627067557No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26110.20711241976475964No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21820.17308284179498487No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT20490.16253287939409902No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.15023781043066056No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG17990.14270212300145638No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC17380.13786341844165156No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA16830.13350065203527017No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG16560.1313589303448648No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16200.12850330142432426No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT16030.12715480998962456No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15890.12604428763163658No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15640.1240612119923723No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC14620.11597026338417411No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA14030.11129020487551043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.027.820971
CGTTTAG358.869873E-426.42857226
TACCGTC1107.4578566E-1121.8636367
GACGTTA603.7266556E-521.5833327
GTATTGG5150.021.5533961
TACCCCG705.102569E-621.1428595
TTGGACC7500.019.4866664
ATTGGAC5150.019.3980583
CGTCTGT3550.018.76056334
CGTATAC801.6168728E-518.53
GGTCTAG902.152552E-618.51
TGGACCC8150.018.3865035
TCTCGAC1451.546141E-1017.86206811
GGACCCT8100.017.586426
ACGGACC953.6069468E-617.5263168
TGCGACG650.001580048717.07692322
GTATCAA63750.017.034512
TAGTACT2300.016.8913044
TATTGGA6650.016.6917292
GTCTAAC904.4465043E-516.4444451
Produced by FastQC (version 0.11.5)