##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1260668
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55549835484045	31.0	31.0	34.0	30.0	34.0
2	31.7204712105011	31.0	31.0	34.0	30.0	34.0
3	31.811738697262086	31.0	31.0	34.0	30.0	34.0
4	35.5285761199618	37.0	35.0	37.0	33.0	37.0
5	35.430456710252024	37.0	35.0	37.0	33.0	37.0
6	35.45796990167118	37.0	35.0	37.0	33.0	37.0
7	35.31216228221864	37.0	35.0	37.0	32.0	37.0
8	35.37802498358013	37.0	35.0	37.0	33.0	37.0
9	36.85909930290925	39.0	37.0	39.0	33.0	39.0
10	36.72890404134951	39.0	35.0	39.0	32.0	39.0
11	36.805393648446696	39.0	37.0	39.0	32.0	39.0
12	36.673210551866156	39.0	35.0	39.0	32.0	39.0
13	36.693069071317744	39.0	35.0	39.0	32.0	39.0
14	37.66685122490616	39.0	36.0	41.0	32.0	41.0
15	37.67124810021354	39.0	36.0	41.0	32.0	41.0
16	37.54430984208372	39.0	36.0	41.0	32.0	41.0
17	37.59523760419079	39.0	36.0	41.0	32.0	41.0
18	37.576833869028164	39.0	36.0	41.0	32.0	41.0
19	37.623325887545334	39.0	37.0	41.0	32.0	41.0
20	37.53744998683238	39.0	36.0	41.0	32.0	41.0
21	37.454260756995495	39.0	36.0	41.0	32.0	41.0
22	37.36293377796533	39.0	36.0	41.0	31.0	41.0
23	37.129850206398515	39.0	36.0	41.0	31.0	41.0
24	36.98156453562714	39.0	36.0	40.0	31.0	41.0
25	36.866160638645546	39.0	36.0	40.0	30.0	41.0
26	36.99607113054349	39.0	36.0	40.0	31.0	41.0
27	36.90249930988968	39.0	35.0	40.0	31.0	41.0
28	36.81497904285664	39.0	35.0	40.0	30.0	41.0
29	36.74144025231068	39.0	35.0	40.0	30.0	41.0
30	36.531930690713175	39.0	35.0	40.0	30.0	41.0
31	36.340835176271625	38.0	35.0	40.0	30.0	41.0
32	36.180370248154155	38.0	35.0	40.0	30.0	41.0
33	35.97216237740626	38.0	35.0	40.0	29.0	41.0
34	35.82740340835176	38.0	35.0	40.0	29.0	41.0
35	35.60622146354155	38.0	34.0	40.0	27.0	41.0
36	35.51381410490311	38.0	34.0	40.0	27.0	41.0
37	35.34132777226042	38.0	34.0	40.0	26.0	41.0
38	35.21341860029762	38.0	34.0	40.0	25.0	41.0
39	35.08952000050767	38.0	34.0	40.0	25.0	41.0
40	34.87016803789737	38.0	33.0	40.0	24.0	41.0
41	34.69468488134862	38.0	33.0	40.0	23.0	41.0
42	34.4599053834951	38.0	33.0	40.0	22.0	41.0
43	34.1281169982898	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	4.0
15	4.0
16	19.0
17	43.0
18	85.0
19	215.0
20	488.0
21	984.0
22	1706.0
23	3024.0
24	4817.0
25	7519.0
26	11201.0
27	15655.0
28	21360.0
29	27864.0
30	35565.0
31	44064.0
32	54412.0
33	66785.0
34	82384.0
35	100688.0
36	130281.0
37	177574.0
38	231111.0
39	242810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.59387562784175	22.922291991230047	13.927378183629632	22.556454197298574
2	18.016876766920394	22.806718342973724	35.973626680458295	23.202778209647583
3	19.796250876519434	25.99447277157824	32.097110420824514	22.11216593107781
4	13.161276402669062	15.686762890784886	35.045229989180335	36.106730717365714
5	11.017968251752245	41.2891419469678	34.17910187297528	13.513787928304676
6	31.754196981282938	36.46035276535932	15.217170579407108	16.568279673950638
7	25.37456332674423	35.20427265544933	20.63588510218392	18.78527891562251
8	28.59484019583269	34.01593440937661	19.440169814733142	17.949055580057557
9	25.72985115827482	13.7032113133672	22.75047831784419	37.81645921051379
10	16.505217868622033	28.19933559033782	32.75263590413971	22.542810636900438
11	32.05126171204473	21.682314455510888	24.540719681946396	21.72570415049799
12	20.187630684684628	27.591086630262684	31.493779488334756	20.72750319671793
13	31.65163230922019	20.71163859160382	28.28230747508464	19.354421624091355
14	20.8941608734417	22.211637005143306	27.99261978570091	28.90158233571408
15	23.543549927498756	28.61601944366003	26.509993114761382	21.330437514079836
16	22.03569853442778	28.497352197406457	26.424165601094025	23.042783667071742
17	19.758572439373413	27.101425593415556	27.43323380937725	25.706768157833782
18	20.827767501039133	26.483102609092956	31.621965497656795	21.06716439221111
19	20.277265703579374	24.65764182163742	32.783730530163375	22.28136194461984
20	24.994764680312343	23.581387010695916	31.528840265636948	19.895008043354792
21	23.92215872854709	24.79534659402793	30.2944946647333	20.988000012691685
22	22.423032868288875	23.967690145224594	31.60253135639201	22.00674563009452
23	21.303784977488124	26.06094546700638	32.604857107501736	20.030412448003755
24	20.095219359894916	25.109148483185106	30.867603524480675	23.92802863243931
25	21.263964818651697	26.316603578420327	31.870405213743823	20.549026389184146
26	22.85867492472245	25.035536715455613	30.553880958349065	21.55190740147287
27	21.44910476033341	26.739395304711472	29.934606097719623	21.876893837235496
28	21.089216193319732	25.778000234796156	30.47931731431273	22.653466257571385
29	20.120920020179778	25.710972278189026	31.73460419396701	22.43350350766419
30	19.516795857434314	26.051268057886773	33.493988901122265	20.937947183556656
31	20.90399692861245	25.552484873099026	32.17167406486085	21.371844133427675
32	22.251695133056444	25.714700460390837	30.145526022711767	21.88807838384095
33	23.252672392731473	25.55621305530084	29.695605821675493	21.495508730292194
34	21.202965411987932	28.350207984973046	29.89970396646857	20.547122636570453
35	19.900402009093593	29.139075474272367	30.243648605342564	20.716873911291476
36	20.874488763100196	25.336329628419218	32.48381017048105	21.305371437999536
37	20.9787985417255	25.80742907728284	31.496159179101873	21.71761320188979
38	21.148153201318664	25.240904028657823	32.38965373912878	21.221289030894734
39	22.310394171978665	24.645029460571696	32.22942122747623	20.81515513997341
40	20.54974029641428	25.494420418381363	31.10517598606453	22.85066329913982
41	21.581653536061836	25.155076514990466	31.00491168174333	22.258358267204372
42	19.154924214781367	26.897803386775898	31.40826926676968	22.53900313167305
43	20.085779919852015	26.463113206649176	30.977307268844772	22.47379960465404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	125.5
2	183.0
3	345.0
4	507.0
5	507.0
6	1051.0
7	1595.0
8	1784.5
9	1974.0
10	3368.0
11	4762.0
12	4762.0
13	9548.5
14	14335.0
15	22768.5
16	31202.0
17	29227.0
18	27252.0
19	27252.0
20	29692.5
21	32133.0
22	25329.5
23	18526.0
24	20659.5
25	22793.0
26	22793.0
27	24951.0
28	27109.0
29	29261.0
30	31413.0
31	34300.5
32	37188.0
33	37188.0
34	40909.0
35	44630.0
36	48484.0
37	52338.0
38	55554.5
39	58771.0
40	58771.0
41	61820.0
42	64869.0
43	74334.5
44	83800.0
45	107185.0
46	130570.0
47	130570.0
48	131172.5
49	131775.0
50	119727.0
51	107679.0
52	100622.0
53	93565.0
54	93565.0
55	81491.5
56	69418.0
57	58155.5
58	46893.0
59	41442.5
60	35992.0
61	35992.0
62	31830.5
63	27669.0
64	24178.5
65	20688.0
66	17766.0
67	14844.0
68	14844.0
69	12389.5
70	9935.0
71	8338.5
72	6742.0
73	5414.5
74	4087.0
75	4087.0
76	3168.0
77	2249.0
78	1789.5
79	1330.0
80	1045.5
81	761.0
82	761.0
83	604.5
84	448.0
85	341.0
86	234.0
87	192.5
88	151.0
89	151.0
90	136.0
91	121.0
92	79.5
93	38.0
94	28.0
95	18.0
96	18.0
97	13.0
98	8.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1260668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.472210677242316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.58402947463999	40.16781902187892
2	11.820940950668675	11.93258044130781
3	3.6441555727448947	5.517857634246809
4	1.5326726891538383	3.094295154649122
5	0.819843848856998	2.0689665730975806
6	0.4915396599918162	1.4885455965196204
7	0.33975856492634454	1.200385610785198
8	0.2446952777051976	0.9880249286450439
9	0.19235844396598906	0.8737880318458119
>10	1.1697715521086263	11.229775840590012
>50	0.0958781894197013	3.359194134730047
>100	0.05145315575376217	4.89537738781427
>500	0.007238055157974628	2.681374115497198
>1k	0.004720470755200845	5.158089252359906
>5k	3.1469805034672297E-4	0.9260878346359275
>10k+	6.293961006934459E-4	4.417838441396739
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16337	1.2959002687464107	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16050	1.2731345604076567	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13053	1.035403452772657	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10188	0.8081429845129724	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6505	0.5159962813365613	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5156	0.4089895198418616	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4706	0.3732941583351049	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4580	0.363299457113213	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4099	0.32514508181376856	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4083	0.32387591340463945	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3780	0.2998410366567566	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3518	0.2790584039572671	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3428	0.27191933165591575	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2709	0.21488607627067557	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2611	0.20711241976475964	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2182	0.17308284179498487	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2049	0.16253287939409902	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1894	0.15023781043066056	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1799	0.14270212300145638	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1738	0.13786341844165156	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1683	0.13350065203527017	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1656	0.1313589303448648	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1620	0.12850330142432426	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1603	0.12715480998962456	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1589	0.12604428763163658	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1564	0.1240612119923723	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1462	0.11597026338417411	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1403	0.11129020487551043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.932302557057052E-5	0.0
5	0.0	0.0	0.0	7.932302557057052E-5	0.0
6	7.932302557057052E-5	0.0	0.0	7.932302557057052E-5	0.0
7	7.932302557057052E-5	0.0	0.0	7.932302557057052E-5	0.0
8	7.932302557057052E-5	0.0	0.0	7.932302557057052E-5	0.0
9	7.932302557057052E-5	0.0	0.0	7.932302557057052E-5	0.0
10	2.3796907671171157E-4	0.0	0.0	3.966151278528526E-4	0.0
11	2.3796907671171157E-4	0.0	0.0	4.7593815342342313E-4	0.0
12	2.3796907671171157E-4	0.0	0.0	9.518763068468463E-4	0.0
13	2.3796907671171157E-4	0.0	0.0	0.0010311993324174167	0.0
14	2.3796907671171157E-4	0.0	0.0	0.00150713748584084	0.0
15	2.3796907671171157E-4	0.0	0.0	0.0023796907671171158	0.0
16	2.3796907671171157E-4	0.0	0.0	0.0037281822018168146	0.0
17	2.3796907671171157E-4	0.0	0.0	0.00610787296893393	0.0
18	3.172921022822821E-4	0.0	0.0	0.0068217801990690646	0.0
19	3.172921022822821E-4	0.0	0.0	0.007456364403633629	0.0
20	3.172921022822821E-4	0.0	0.0	0.010074024247462456	0.0
21	3.172921022822821E-4	0.0	0.0	0.014436790653843835	0.0
22	3.172921022822821E-4	0.0	0.0	0.026335244489429414	0.0
23	3.172921022822821E-4	0.0	0.0	0.042993079859249224	0.0
24	3.172921022822821E-4	0.0	0.0	0.06425165071216213	0.0
25	4.7593815342342313E-4	0.0	0.0	0.07258056839707203	0.0
26	4.7593815342342313E-4	0.0	0.0	0.08114745515869365	0.0
27	4.7593815342342313E-4	0.0	0.0	0.12041235281612606	7.932302557057052E-5
28	4.7593815342342313E-4	0.0	0.0	0.19370682844333323	7.932302557057052E-5
29	4.7593815342342313E-4	0.0	0.0	0.2831038782613662	7.932302557057052E-5
30	4.7593815342342313E-4	0.0	0.0	0.4540449983659457	7.932302557057052E-5
31	4.7593815342342313E-4	0.0	0.0	0.6491003182439786	7.932302557057052E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3910	0.0	27.82097	1
CGTTTAG	35	8.869873E-4	26.428572	26
TACCGTC	110	7.4578566E-11	21.863636	7
GACGTTA	60	3.7266556E-5	21.583332	7
GTATTGG	515	0.0	21.553396	1
TACCCCG	70	5.102569E-6	21.142859	5
TTGGACC	750	0.0	19.486666	4
ATTGGAC	515	0.0	19.398058	3
CGTCTGT	355	0.0	18.760563	34
CGTATAC	80	1.6168728E-5	18.5	3
GGTCTAG	90	2.152552E-6	18.5	1
TGGACCC	815	0.0	18.386503	5
TCTCGAC	145	1.546141E-10	17.862068	11
GGACCCT	810	0.0	17.58642	6
ACGGACC	95	3.6069468E-6	17.526316	8
TGCGACG	65	0.0015800487	17.076923	22
GTATCAA	6375	0.0	17.03451	2
TAGTACT	230	0.0	16.891304	4
TATTGGA	665	0.0	16.691729	2
GTCTAAC	90	4.4465043E-5	16.444445	1
>>END_MODULE