FastQCFastQC Report
Wed 25 May 2016
SRR2088132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2563704
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT328411.280998118347516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT312871.2203826962863107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT244520.9537762549810742No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG164200.6404795561422068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125980.491398382964648No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA97530.3804261334381816No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT92340.36018198668801077No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT85690.33424295472488247No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA75410.2941447218555652No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA73570.2869676062447147No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG67650.26387601688806506No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT61070.2382100273666539No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT60160.23466047562433107No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA48970.191012691012691No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47890.18680003619762656No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT40520.15805256769112191No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA40380.15750648280768764No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39320.15337184011882807No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35720.13932965740194656No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.1282129294177487No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA32740.12770585059741685No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT32060.12505343830645035No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30410.11861743789454632No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC29810.11627707410839941No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG29650.11565297709876023No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC28580.11147932834679823No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG28490.11112827377887619No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA26950.1051213400610991No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA25750.10044061248880526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80750.027.560991
ATTGGAC10350.021.2705333
GTATTGG10500.021.1428571
CGCGATA902.1543183E-618.514
GTATCAA130250.017.0299432
TATTGGA13600.016.8676452
TTGGACC17150.016.7201164
TGGACCC17750.015.9464785
GGACCCT17700.015.6779666
TGCGACG1902.7284841E-1115.57894822
TGCGTTA957.0650916E-515.57894837
ACGTGCG1201.937664E-615.4166679
CAATGCG1954.1836756E-1115.17948719
CGTCTGT7100.014.85211334
ATTCGTC1252.9620005E-614.80000117
TACTAGG3300.014.5757572
ACGTTTA1803.3360266E-914.38888826
CGCGAAT908.2789117E-414.38888835
TTTTTAC14600.014.0650681
ATACACT4250.013.9294124
Produced by FastQC (version 0.11.5)