##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2563704
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.471397634048238	31.0	31.0	34.0	30.0	34.0
2	31.64881905243351	31.0	31.0	34.0	30.0	34.0
3	31.734793096238878	31.0	31.0	34.0	30.0	34.0
4	35.46229166861697	37.0	35.0	37.0	33.0	37.0
5	35.356453787176676	37.0	35.0	37.0	33.0	37.0
6	35.39007779369225	37.0	35.0	37.0	33.0	37.0
7	35.24749230020314	37.0	35.0	37.0	32.0	37.0
8	35.31023784337037	37.0	35.0	37.0	32.0	37.0
9	36.77546978902401	39.0	37.0	39.0	32.0	39.0
10	36.629076913715465	38.0	35.0	39.0	32.0	39.0
11	36.709187566115276	39.0	35.0	39.0	32.0	39.0
12	36.58428703157619	38.0	35.0	39.0	32.0	39.0
13	36.597046694938264	38.0	35.0	39.0	32.0	39.0
14	37.55271942470738	39.0	36.0	41.0	32.0	41.0
15	37.56764548481416	39.0	36.0	41.0	32.0	41.0
16	37.439796091904526	39.0	36.0	41.0	32.0	41.0
17	37.48181420319975	39.0	36.0	41.0	32.0	41.0
18	37.46420296570899	39.0	36.0	41.0	32.0	41.0
19	37.50948510436462	39.0	36.0	41.0	32.0	41.0
20	37.430245847414525	39.0	36.0	41.0	32.0	41.0
21	37.35198330228451	39.0	36.0	41.0	31.0	41.0
22	37.23893202959468	39.0	36.0	41.0	31.0	41.0
23	37.018513447730314	39.0	36.0	40.0	31.0	41.0
24	36.85615266036953	39.0	36.0	40.0	30.0	41.0
25	36.75396262595058	39.0	35.0	40.0	30.0	41.0
26	36.877009202310404	39.0	36.0	40.0	31.0	41.0
27	36.7639431073166	39.0	35.0	40.0	30.0	41.0
28	36.66916071434144	39.0	35.0	40.0	30.0	41.0
29	36.579906650689786	39.0	35.0	40.0	30.0	41.0
30	36.37221457703385	38.0	35.0	40.0	30.0	41.0
31	36.1859321512936	38.0	35.0	40.0	30.0	41.0
32	36.024687717458804	38.0	35.0	40.0	29.0	41.0
33	35.80175324452433	38.0	35.0	40.0	29.0	41.0
34	35.65961866112468	38.0	34.0	40.0	27.0	41.0
35	35.439973959552276	38.0	34.0	40.0	27.0	41.0
36	35.34054672458287	38.0	34.0	40.0	26.0	41.0
37	35.17379346445612	38.0	34.0	40.0	25.0	41.0
38	35.03835232148485	38.0	34.0	40.0	25.0	41.0
39	34.91688159007436	38.0	33.0	40.0	24.0	41.0
40	34.69261935075188	38.0	33.0	40.0	24.0	41.0
41	34.51419664672677	38.0	33.0	40.0	23.0	41.0
42	34.29605796925074	38.0	33.0	40.0	22.0	41.0
43	33.96156849620705	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	10.0
13	13.0
14	8.0
15	16.0
16	53.0
17	129.0
18	252.0
19	576.0
20	1165.0
21	2224.0
22	4063.0
23	6629.0
24	11042.0
25	16895.0
26	24182.0
27	34203.0
28	45913.0
29	59789.0
30	75076.0
31	93302.0
32	113708.0
33	139440.0
34	170685.0
35	207683.0
36	267550.0
37	360938.0
38	475442.0
39	452714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.078924868081494	23.393613303251858	14.27075824666186	22.256703582004786
2	18.174172993450103	23.318682656032053	35.70065030908404	22.8064940414338
3	19.953278537615887	26.028355847632955	32.29389976377928	21.724465850971875
4	13.538341399787182	16.08610042344982	35.1377148063895	35.23784337037349
5	11.116962020576478	41.20916455253805	34.26655339305942	13.407320033826059
6	31.627247139295335	36.8102167800963	15.193095614782361	16.369440465826006
7	24.874088428305296	35.29639147109027	21.011122968954293	18.818397131650144
8	28.18004730655333	34.296080982827974	19.729071686903012	17.794800023715688
9	25.47450875764129	13.90913303563906	23.016853739745304	37.599504466974345
10	16.6129943238377	28.379368289006845	32.83464081656853	22.172996570586932
11	32.106631654824426	21.97738116412815	24.305224004019184	21.610763177028236
12	19.926949445021734	27.816939865132635	31.38895909980247	20.867151590043157
13	31.13514664719484	21.000669344042837	28.335642492269	19.528541516493323
14	20.716900234972524	22.579361735988243	28.075160002870845	28.628578026168388
15	23.35413916739218	29.230324561649862	26.114091174332138	21.30144509662582
16	21.98065767342876	28.600025587977395	26.458709741842274	22.960606996751576
17	19.716706764899534	27.286457406939334	27.70690376112063	25.2899320670405
18	20.783795633193222	26.601588950986542	31.64702321328827	20.96759220253196
19	20.17584713367846	25.009517479396997	32.71668648174672	22.097948905177823
20	24.604010447383942	24.07879380770947	31.453514134237025	19.863681610669563
21	23.891720729070126	24.912314370145697	30.27408780420829	20.92187709657589
22	22.287401353666414	24.397941416013705	31.295773615050727	22.018883615269157
23	21.153651123530643	26.407572793114966	32.31262267406846	20.12615340928594
24	20.098303080230792	25.590941856002097	30.808821923279755	23.501933140487356
25	21.2008094538215	26.550998087142663	31.540146600387565	20.70804585864827
26	22.55252556457376	25.50212505031782	30.415718819333275	21.529630565775143
27	21.37235811934607	26.913910498247844	29.98665212520634	21.727079257199737
28	21.01853412094376	26.340716400957366	30.275023949722744	22.36572552837613
29	20.033396991228315	26.190777094391553	31.47106686263313	22.304759051747002
30	19.502953539098115	26.403048089795078	33.07152463778969	21.022473733317106
31	20.816989792893406	25.805904269759694	32.09719218755364	21.279913749793266
32	21.93736094338504	26.03955058774336	30.0831921313849	21.9398963374867
33	22.805011811035907	25.92733014419761	29.765799795920277	21.5018582488462
34	21.137931680100355	28.44848703282438	29.953301941253745	20.460279345821515
35	20.009135219978592	29.06926852709985	30.209220721268913	20.71237553165264
36	20.587556129724803	25.6436000411904	32.36754321091671	21.401300618168086
37	20.768115195826038	26.032022417564587	31.526260441923093	21.673601944686283
38	21.019782314963038	25.332253645506658	32.38064924811913	21.267314791411177
39	22.051804732527625	24.936459123206113	32.27876541129553	20.73297073297073
40	20.41257493064722	25.541521174051297	31.45402121305736	22.591882682244126
41	21.145225813900513	25.325700626905444	31.26020008550129	22.26887347369275
42	19.071429462995727	26.8239235106705	31.660909371752744	22.443737654581028
43	19.654453088188028	26.54561525043453	31.135068635068635	22.664863026308808
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	259.0
2	369.0
3	747.0
4	1125.0
5	1125.0
6	2131.0
7	3137.0
8	3832.0
9	4527.0
10	7238.5
11	9950.0
12	9950.0
13	19636.0
14	29322.0
15	47016.5
16	64711.0
17	59917.0
18	55123.0
19	55123.0
20	60689.0
21	66255.0
22	53719.5
23	41184.0
24	45817.0
25	50450.0
26	50450.0
27	55644.0
28	60838.0
29	64524.0
30	68210.0
31	73518.0
32	78826.0
33	78826.0
34	84737.5
35	90649.0
36	99284.0
37	107919.0
38	114426.5
39	120934.0
40	120934.0
41	127437.0
42	133940.0
43	151260.0
44	168580.0
45	211651.5
46	254723.0
47	254723.0
48	258819.0
49	262915.0
50	239284.5
51	215654.0
52	202907.5
53	190161.0
54	190161.0
55	165203.5
56	140246.0
57	117985.5
58	95725.0
59	84417.5
60	73110.0
61	73110.0
62	64129.0
63	55148.0
64	47664.5
65	40181.0
66	34225.5
67	28270.0
68	28270.0
69	23775.5
70	19281.0
71	16164.0
72	13047.0
73	10530.5
74	8014.0
75	8014.0
76	6236.0
77	4458.0
78	3538.5
79	2619.0
80	2089.5
81	1560.0
82	1560.0
83	1240.5
84	921.0
85	700.5
86	480.0
87	435.5
88	391.0
89	391.0
90	376.5
91	362.0
92	241.5
93	121.0
94	97.5
95	74.0
96	74.0
97	47.0
98	20.0
99	22.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2563704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.98520440003989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.88346511300857	33.37749723578978
2	12.883439621549744	11.33361450258876
3	4.689826913404343	6.188489861606948
4	2.1800386171073183	3.835577766937828
5	1.1314069137763807	2.4882582181036206
6	0.6759395629094198	1.7838803901986673
7	0.4405045131184612	1.3562976734058902
8	0.3153381656391145	1.109617093661607
9	0.2144445182494373	0.8489147370902626
>10	1.3323187576637283	11.17280070961642
>50	0.14367085823742076	4.449541378049413
>100	0.0964348676207285	7.996415635821838
>500	0.006229800270784798	1.9333608973863419
>1k	0.005784814537157265	5.121024369719568
>5k	7.11977173803971E-4	2.401267978041108
>10k+	4.4498573362748185E-4	4.603441551981973
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32841	1.280998118347516	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31287	1.2203826962863107	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24452	0.9537762549810742	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16420	0.6404795561422068	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12598	0.491398382964648	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9753	0.3804261334381816	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9234	0.36018198668801077	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8569	0.33424295472488247	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7541	0.2941447218555652	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7357	0.2869676062447147	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6765	0.26387601688806506	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6107	0.2382100273666539	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6016	0.23466047562433107	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4897	0.191012691012691	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4789	0.18680003619762656	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4052	0.15805256769112191	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4038	0.15750648280768764	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3932	0.15337184011882807	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3572	0.13932965740194656	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3287	0.1282129294177487	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3274	0.12770585059741685	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3206	0.12505343830645035	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3041	0.11861743789454632	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2981	0.11627707410839941	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2965	0.11565297709876023	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2858	0.11147932834679823	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2849	0.11112827377887619	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2695	0.1051213400610991	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2575	0.10044061248880526	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.9006063102448646E-5	0.0
11	0.0	0.0	0.0	1.5602425240979459E-4	0.0
12	0.0	0.0	0.0	4.6807275722938373E-4	0.0
13	0.0	0.0	0.0	7.021091358440756E-4	0.0
14	0.0	0.0	0.0	8.971394513563188E-4	0.0
15	0.0	0.0	0.0	0.001755272839610189	0.0
16	0.0	0.0	0.0	0.0025744001647616107	0.0
17	0.0	0.0	0.0	0.004524703319884043	0.0
18	0.0	0.0	0.0	0.005226812455728118	0.0
19	0.0	0.0	0.0	0.006436000411904026	0.0
20	0.0	0.0	0.0	0.008269285377719112	0.0
21	0.0	0.0	0.0	0.011896849246246836	0.0
22	0.0	0.0	0.0	0.02106327407532227	0.0
23	0.0	0.0	0.0	0.033857262772925424	0.0
24	0.0	0.0	0.0	0.05070788203318324	0.0
25	0.0	0.0	0.0	0.05729990669749706	0.0
26	0.0	0.0	0.0	0.06572521632762597	0.0
27	0.0	0.0	0.0	0.11144032228369578	0.0
28	0.0	0.0	0.0	0.1913247395175106	0.0
29	0.0	0.0	0.0	0.292038394448033	0.0
30	0.0	0.0	0.0	0.4684628178604082	0.0
31	0.0	0.0	0.0	0.6980134992183185	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8075	0.0	27.56099	1
ATTGGAC	1035	0.0	21.270533	3
GTATTGG	1050	0.0	21.142857	1
CGCGATA	90	2.1543183E-6	18.5	14
GTATCAA	13025	0.0	17.029943	2
TATTGGA	1360	0.0	16.867645	2
TTGGACC	1715	0.0	16.720116	4
TGGACCC	1775	0.0	15.946478	5
GGACCCT	1770	0.0	15.677966	6
TGCGACG	190	2.7284841E-11	15.578948	22
TGCGTTA	95	7.0650916E-5	15.578948	37
ACGTGCG	120	1.937664E-6	15.416667	9
CAATGCG	195	4.1836756E-11	15.179487	19
CGTCTGT	710	0.0	14.852113	34
ATTCGTC	125	2.9620005E-6	14.800001	17
TACTAGG	330	0.0	14.575757	2
ACGTTTA	180	3.3360266E-9	14.388888	26
CGCGAAT	90	8.2789117E-4	14.388888	35
TTTTTAC	1460	0.0	14.065068	1
ATACACT	425	0.0	13.929412	4
>>END_MODULE