FastQCFastQC Report
Wed 25 May 2016
SRR2088131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534424
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT203310.8021941080103407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT197710.7800983576544414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT163000.6431441621449292No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG115900.4573031189729895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82560.3257544909612598No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA62700.24739349059194515No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT58540.23097950461327701No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT55150.21760368430854504No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA53830.2123954002960831No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA47640.1879717048134014No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG44900.17716056981783634No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT40870.16125952090100157No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT38090.1502905591171801No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33700.1329690691060375No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA30070.118646288071767No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA28830.11375365763581784No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT26580.10487590079639396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45400.029.58371
ATTGGAC8050.019.7639753
GTATTGG8750.018.6057151
GTATCAA78300.017.0823752
TTGGACC12400.016.8588714
ACGTTTA1352.222805E-816.44444526
GGACCCT12950.016.4285726
AACCGTG855.367321E-415.2352937
TATTGGA10600.015.1839632
GGCGCTA1101.4531628E-515.13636410
GTACTAG2450.015.102041
TTTACAC4450.014.9662913
TGGACCC13450.014.8550195
CGGTCGT750.0041062214.822
GAACCGA908.2788646E-414.3888896
CGACTAT908.2788646E-414.38888936
GTCGTAA1304.4487624E-614.2307691
GACCCTC14950.013.9832787
GTACTGT3900.013.7564096
TAACGCC2202.8558134E-1013.4545454
Produced by FastQC (version 0.11.5)