##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088131_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2534424
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.663084787707188	31.0	31.0	34.0	30.0	34.0
2	31.85464665738645	31.0	31.0	34.0	30.0	34.0
3	31.956954716337915	31.0	31.0	34.0	30.0	34.0
4	35.65464144910244	37.0	35.0	37.0	33.0	37.0
5	35.53952811368579	37.0	35.0	37.0	33.0	37.0
6	35.55163263921112	37.0	35.0	37.0	33.0	37.0
7	35.42983612844575	37.0	35.0	37.0	33.0	37.0
8	35.45725853290531	37.0	35.0	37.0	33.0	37.0
9	37.00646774178275	39.0	37.0	39.0	33.0	39.0
10	36.8514056842896	39.0	37.0	39.0	33.0	39.0
11	36.91447011234111	39.0	37.0	39.0	33.0	39.0
12	36.79820148483442	39.0	37.0	39.0	32.0	39.0
13	36.806755696757925	39.0	37.0	39.0	32.0	39.0
14	37.860679191800585	40.0	37.0	41.0	33.0	41.0
15	37.86168336474086	40.0	37.0	41.0	33.0	41.0
16	37.75284798439409	40.0	37.0	41.0	32.0	41.0
17	37.72433933706436	39.0	37.0	41.0	32.0	41.0
18	37.69492594767095	39.0	37.0	41.0	32.0	41.0
19	37.71222376366386	40.0	37.0	41.0	32.0	41.0
20	37.62483033620263	39.0	37.0	41.0	32.0	41.0
21	37.537659049945866	39.0	36.0	41.0	32.0	41.0
22	37.42459983017837	39.0	36.0	41.0	32.0	41.0
23	37.210052461624414	39.0	36.0	41.0	31.0	41.0
24	37.0580932787884	39.0	36.0	41.0	31.0	41.0
25	36.94518833470643	39.0	36.0	40.0	31.0	41.0
26	37.12673925120659	39.0	36.0	41.0	31.0	41.0
27	37.04631111447808	39.0	36.0	41.0	31.0	41.0
28	36.98005700703592	39.0	36.0	41.0	31.0	41.0
29	36.89777598381328	39.0	36.0	41.0	30.0	41.0
30	36.7226655839749	39.0	35.0	40.0	30.0	41.0
31	36.568217472688076	39.0	35.0	40.0	30.0	41.0
32	36.42698183098014	39.0	35.0	40.0	30.0	41.0
33	36.237513533647096	38.0	35.0	40.0	30.0	41.0
34	36.11990416757417	38.0	35.0	40.0	29.0	41.0
35	35.92513131188783	38.0	35.0	40.0	28.0	41.0
36	35.840533391413594	38.0	35.0	40.0	28.0	41.0
37	35.6999476015063	38.0	35.0	40.0	27.0	41.0
38	35.61647616973324	38.0	35.0	40.0	27.0	41.0
39	35.536309630906274	38.0	34.0	40.0	26.0	41.0
40	35.36939675444992	38.0	34.0	40.0	25.0	41.0
41	35.23781143170993	38.0	34.0	40.0	25.0	41.0
42	35.072684365362704	38.0	34.0	40.0	24.0	41.0
43	34.76292088458758	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	8.0
13	10.0
14	11.0
15	18.0
16	59.0
17	110.0
18	197.0
19	427.0
20	927.0
21	1805.0
22	3205.0
23	5682.0
24	9121.0
25	13767.0
26	20035.0
27	28617.0
28	38519.0
29	50565.0
30	64991.0
31	81782.0
32	101207.0
33	126711.0
34	156944.0
35	195773.0
36	252381.0
37	346753.0
38	485956.0
39	548842.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.87066726009539	22.131064099771784	13.217677862899023	23.7805907772338
2	18.31366811551658	22.427226067934964	35.54180358140548	23.717302235142977
3	19.31937986698358	25.414532059355498	31.526098237706083	23.739989835954837
4	13.611179502719356	15.86940464578934	35.66123900341853	34.85817684807277
5	11.990101103840557	39.77751946793433	34.81702351303492	13.415355915190197
6	32.44855635836782	37.00398986120712	15.328177132160997	15.219276648264064
7	26.36259757641184	33.588184139670396	21.532427091915167	18.5167911920026
8	27.513075949407046	34.45800702644861	19.858674002455786	18.170243021688556
9	25.796157233359533	14.072309921307562	21.872820017487207	38.2587128278457
10	16.22822384889032	27.892452091678425	33.629810955073026	22.24951310435823
11	33.16481378017254	22.257956837529946	23.557897179003987	21.01933220329353
12	20.123507353150064	27.07569846245143	31.236367711164352	21.564426473234157
13	30.563433742736024	20.983821175935834	27.051787704030584	21.400957377297562
14	21.11935493035104	22.026622222643095	27.152520651635243	29.701502195370626
15	23.442446883394414	29.220761798341556	24.86967452959726	22.467116788666775
16	22.924538277730953	28.254822397515174	25.536255969798265	23.284383354955605
17	20.8389756410135	27.33323232418885	26.989327752578102	24.83846428221955
18	21.75646221784516	26.807156182233122	29.63493874742348	21.80144285249824
19	21.4810544723377	25.438837384746986	30.449522258311944	22.630585884603367
20	24.436005972165667	25.05969798265799	29.345997354823027	21.158298690353313
21	23.916203445043134	25.588220439831694	28.57154130484875	21.924034810276417
22	22.918185749503632	25.16855900985786	29.391372556446747	22.521882684191752
23	22.177228435336787	26.430897119029805	30.011986944568076	21.37988750106533
24	21.44885780753339	25.87013854035473	28.844818388714756	23.836185263397127
25	22.379799118063907	26.4114449673772	29.506822852056324	21.701933062502565
26	23.041843038102545	26.020429099471908	28.620349239117054	22.317378623308493
27	22.171625584353684	26.937915676303568	28.363525597926788	22.52693314141596
28	22.15939400826381	26.31213246086685	28.65901680223988	22.869456728629466
29	21.35546380558265	26.26119386495709	29.516253002654647	22.86708932680562
30	21.011559233971898	26.592511750204384	30.40189802495557	21.99403099086814
31	21.983930076419732	26.056295237103182	29.85384450273514	22.105930183741947
32	22.653352398809353	26.119307582314562	28.562663548009333	22.664676470866755
33	23.19477719592302	25.959271219022543	28.455380788692025	22.390570796362407
34	22.17466375002762	27.51599574498979	28.551260562557808	21.75807994242479
35	21.35846251455952	28.120945824376665	28.80559843183303	21.714993229230785
36	21.90667386356821	25.707616405147675	30.038738585177537	22.34697114610657
37	21.940843363225724	25.73531500648668	29.703632857012085	22.620208773275504
38	21.949721120065153	25.497351666493056	30.439736997440047	22.113190216001744
39	22.55285619138708	25.197086201835212	30.384260881367915	21.865796725409798
40	21.477384999510736	25.404983538665988	30.041303270486708	23.07632819133657
41	21.887497908795055	25.13865083348327	29.995651871983537	22.97819938573814
42	20.36549527624423	26.356205591487452	30.30408487293365	22.974214259334666
43	20.81893163890493	25.97813152021919	29.99588861216592	23.207048228709954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	147.0
1	258.0
2	369.0
3	669.5
4	970.0
5	970.0
6	1735.0
7	2500.0
8	2798.5
9	3097.0
10	5033.5
11	6970.0
12	6970.0
13	13468.0
14	19966.0
15	30703.5
16	41441.0
17	39697.0
18	37953.0
19	37953.0
20	42613.5
21	47274.0
22	41042.5
23	34811.0
24	40421.0
25	46031.0
26	46031.0
27	51473.0
28	56915.0
29	62855.5
30	68796.0
31	76065.5
32	83335.0
33	83335.0
34	91976.0
35	100617.0
36	109757.0
37	118897.0
38	125818.5
39	132740.0
40	132740.0
41	139093.5
42	145447.0
43	158375.0
44	171303.0
45	198492.0
46	225681.0
47	225681.0
48	227776.5
49	229872.0
50	213961.5
51	198051.0
52	187941.5
53	177832.0
54	177832.0
55	161613.5
56	145395.0
57	127458.0
58	109521.0
59	100522.5
60	91524.0
61	91524.0
62	81575.0
63	71626.0
64	62820.5
65	54015.0
66	45999.0
67	37983.0
68	37983.0
69	32280.0
70	26577.0
71	22746.0
72	18915.0
73	15393.5
74	11872.0
75	11872.0
76	9345.0
77	6818.0
78	5312.0
79	3806.0
80	3065.5
81	2325.0
82	2325.0
83	1812.0
84	1299.0
85	976.0
86	653.0
87	555.5
88	458.0
89	458.0
90	417.5
91	377.0
92	256.0
93	135.0
94	101.0
95	67.0
96	67.0
97	45.0
98	23.0
99	21.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2534424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.74452272749993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68133342486598	38.14476333328245
2	12.884202252501197	12.818369835505033
3	4.434411740581958	6.617630868374152
4	2.0059623348666227	3.9914255582912608
5	1.0687070182113112	2.658116027822562
6	0.6203050532220394	1.8514067290792076
7	0.3852967890739157	1.3416483416644098
8	0.285949922625926	1.1379553939993776
9	0.20531596641699748	0.9192010281974047
>10	1.2295320381883208	11.59425966299674
>50	0.12449673214550346	4.288199158952007
>100	0.0661291930425205	5.9996963434601955
>500	0.004377756039809892	1.5752645409044634
>1k	0.0032634181387666402	3.131523753678875
>5k	3.9797782180080974E-4	1.2384346942415962
>10k+	3.1838225744064777E-4	2.6921047295503104
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20331	0.8021941080103407	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19771	0.7800983576544414	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16300	0.6431441621449292	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11590	0.4573031189729895	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8256	0.3257544909612598	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6270	0.24739349059194515	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5854	0.23097950461327701	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5515	0.21760368430854504	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5383	0.2123954002960831	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4764	0.1879717048134014	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4490	0.17716056981783634	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4087	0.16125952090100157	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3809	0.1502905591171801	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3370	0.1329690691060375	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3007	0.118646288071767	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2883	0.11375365763581784	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2658	0.10487590079639396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.9456697064106086E-5	0.0
3	0.0	0.0	0.0	7.891339412821217E-5	0.0
4	0.0	0.0	0.0	7.891339412821217E-5	0.0
5	0.0	0.0	0.0	7.891339412821217E-5	0.0
6	0.0	0.0	0.0	7.891339412821217E-5	0.0
7	0.0	0.0	0.0	1.1837009119231826E-4	0.0
8	0.0	0.0	0.0	1.1837009119231826E-4	0.0
9	0.0	0.0	0.0	1.5782678825642434E-4	0.0
10	0.0	0.0	0.0	1.9728348532053043E-4	0.0
11	0.0	0.0	0.0	2.761968794487426E-4	0.0
12	0.0	0.0	0.0	3.9456697064106086E-4	0.0
13	0.0	0.0	0.0	4.3402366770516694E-4	0.0
14	0.0	0.0	0.0	4.7348036476927303E-4	0.0
15	0.0	0.0	0.0	7.891339412821217E-4	0.0
16	3.9456697064106086E-5	0.0	0.0	0.0014993544884360313	0.0
17	3.9456697064106086E-5	0.0	0.0	0.0022490317326540467	0.0
18	3.9456697064106086E-5	0.0	0.0	0.002761968794487426	0.0
19	3.9456697064106086E-5	0.0	0.0	0.003274905856320805	0.0
20	3.9456697064106086E-5	0.0	0.0	0.004616433556500412	0.0
21	3.9456697064106086E-5	0.0	0.0	0.006470898318513398	0.0
22	3.9456697064106086E-5	0.0	0.0	0.010968961783821491	0.0
23	3.9456697064106086E-5	0.0	0.0	0.01937323825847609	0.0
24	3.9456697064106086E-5	0.0	0.0	0.02994763307165652	0.0
25	3.9456697064106086E-5	0.0	0.0	0.03555048405475958	0.0
26	3.9456697064106086E-5	0.0	0.0	0.040956051552542116	0.0
27	3.9456697064106086E-5	0.0	0.0	0.08005763834307125	0.0
28	3.9456697064106086E-5	0.0	0.0	0.1446482514370129	0.0
29	3.9456697064106086E-5	0.0	0.0	0.22841481930411012	0.0
30	3.9456697064106086E-5	0.0	0.0	0.3689201175493919	0.0
31	3.9456697064106086E-5	0.0	0.0	0.5627314135282809	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4540	0.0	29.5837	1
ATTGGAC	805	0.0	19.763975	3
GTATTGG	875	0.0	18.605715	1
GTATCAA	7830	0.0	17.082375	2
TTGGACC	1240	0.0	16.858871	4
ACGTTTA	135	2.222805E-8	16.444445	26
GGACCCT	1295	0.0	16.428572	6
AACCGTG	85	5.367321E-4	15.235293	7
TATTGGA	1060	0.0	15.183963	2
GGCGCTA	110	1.4531628E-5	15.136364	10
GTACTAG	245	0.0	15.10204	1
TTTACAC	445	0.0	14.966291	3
TGGACCC	1345	0.0	14.855019	5
CGGTCGT	75	0.00410622	14.8	22
GAACCGA	90	8.2788646E-4	14.388889	6
CGACTAT	90	8.2788646E-4	14.388889	36
GTCGTAA	130	4.4487624E-6	14.230769	1
GACCCTC	1495	0.0	13.983278	7
GTACTGT	390	0.0	13.756409	6
TAACGCC	220	2.8558134E-10	13.454545	4
>>END_MODULE