FastQCFastQC Report
Wed 25 May 2016
SRR2088130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716611
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT76791.0715716057944966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74051.0333360777325495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58470.8159238415262954No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG33820.47194363469162487No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28420.3965889443505612No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21580.3011396699185472No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT20420.28495236606750385No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT20260.28271963450184273No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA19800.2763005312505669No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA16920.23611136306866629No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG14440.2015040238009185No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT14400.2009458409095032No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT12100.16885032465312422No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA11000.15350029513920385No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA10060.1403829971909446No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA9730.1357779883367685No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT9100.12698660779697774No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.12210250749709395No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8510.11875341014860223No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA8330.11624158713723345No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC7830.10926430099454237No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG7720.10772929804315032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.030.2930531
TTCCGCC358.8661216E-426.42857231
ATTGGAC1950.021.8205133
TATACTC705.0974995E-621.1428595
TGCGGCG450.003824422420.55555736
GTATTGG2150.019.7906971
TTGGACC3750.019.7333354
GTATTAG1103.8455255E-818.51
GGACCCT3900.018.56
GTATCAA27850.018.0017972
GACCCTC3950.017.7974687
TGCGCAG752.065898E-417.26666610
GACGGAC650.001579149417.0769237
ACTGGTC650.001579149417.0769238
TATTGGA2650.016.7547172
TGGACCC4100.016.6951225
GCTTCGG803.3803433E-416.187533
TTTTTAC3000.016.0333331
CGCGCGT700.00259121615.8571437
ACGGACC700.00259121615.8571438
Produced by FastQC (version 0.11.5)