##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088130_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	716611
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.570546642460137	31.0	31.0	34.0	30.0	34.0
2	31.763771418524136	31.0	31.0	34.0	30.0	34.0
3	31.85606137779074	31.0	31.0	34.0	30.0	34.0
4	35.569956364052466	37.0	35.0	37.0	33.0	37.0
5	35.45362965402429	37.0	35.0	37.0	33.0	37.0
6	35.47121520601833	37.0	35.0	37.0	33.0	37.0
7	35.33198764741261	37.0	35.0	37.0	32.0	37.0
8	35.37839776391934	37.0	35.0	37.0	33.0	37.0
9	36.89979779824758	39.0	37.0	39.0	33.0	39.0
10	36.75178723184545	39.0	35.0	39.0	32.0	39.0
11	36.81272824447294	39.0	37.0	39.0	32.0	39.0
12	36.68983172181281	39.0	35.0	39.0	32.0	39.0
13	36.70477148690154	39.0	35.0	39.0	32.0	39.0
14	37.70795731575429	39.0	37.0	41.0	32.0	41.0
15	37.713396808031135	39.0	37.0	41.0	32.0	41.0
16	37.5978543449654	39.0	36.0	41.0	32.0	41.0
17	37.60884077972568	39.0	36.0	41.0	32.0	41.0
18	37.58833593120954	39.0	36.0	41.0	32.0	41.0
19	37.62376798569935	39.0	37.0	41.0	32.0	41.0
20	37.52831592035288	39.0	36.0	41.0	32.0	41.0
21	37.45690897851136	39.0	36.0	41.0	32.0	41.0
22	37.34301455043252	39.0	36.0	41.0	31.0	41.0
23	37.125346945553446	39.0	36.0	41.0	31.0	41.0
24	36.9724606515948	39.0	36.0	40.0	31.0	41.0
25	36.865934237682644	39.0	36.0	40.0	30.0	41.0
26	37.004659431686086	39.0	36.0	40.0	31.0	41.0
27	36.90283710409134	39.0	36.0	41.0	31.0	41.0
28	36.83493415535067	39.0	35.0	41.0	30.0	41.0
29	36.73607019708042	39.0	35.0	40.0	30.0	41.0
30	36.55186147017001	39.0	35.0	40.0	30.0	41.0
31	36.376050604860936	38.0	35.0	40.0	30.0	41.0
32	36.220458519336155	38.0	35.0	40.0	30.0	41.0
33	36.014483450575	38.0	35.0	40.0	29.0	41.0
34	35.89075663086388	38.0	35.0	40.0	29.0	41.0
35	35.68556022723625	38.0	35.0	40.0	27.0	41.0
36	35.60078061877365	38.0	34.0	40.0	27.0	41.0
37	35.440223496429724	38.0	34.0	40.0	26.0	41.0
38	35.33299377207439	38.0	34.0	40.0	25.0	41.0
39	35.23421493669508	38.0	34.0	40.0	25.0	41.0
40	35.05660672247565	38.0	34.0	40.0	24.0	41.0
41	34.908079836898956	38.0	34.0	40.0	24.0	41.0
42	34.7095760461394	38.0	33.0	40.0	23.0	41.0
43	34.391841598859074	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	0.0
14	1.0
15	0.0
16	18.0
17	24.0
18	63.0
19	116.0
20	274.0
21	566.0
22	1023.0
23	1779.0
24	2825.0
25	4363.0
26	6238.0
27	8848.0
28	11838.0
29	15559.0
30	19819.0
31	24999.0
32	29874.0
33	37511.0
34	45743.0
35	56240.0
36	72506.0
37	99110.0
38	132451.0
39	144819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.72517865341169	22.91717542711457	14.366790350692357	22.99085556878139
2	18.05316971132176	22.66376039441203	36.32626348186115	22.956806412405058
3	19.535145288029348	25.4086247629467	32.13570542456088	22.92052452446306
4	13.641989866189608	16.12757828166188	35.402889433737414	34.827542418411106
5	11.677744271299211	40.21386777484577	34.6118047308791	13.496583222975925
6	31.93210821491716	37.04352849732979	15.159410056502063	15.864953231250986
7	25.350713287962368	34.51314590482145	21.44747987401812	18.68866093319807
8	27.26597833413107	34.896896642669454	19.780327123083513	18.05679790011596
9	25.24005352973929	14.488195129575182	22.745255096558665	37.52649624412687
10	16.753301302938414	27.913749579618507	33.27216579148241	22.060783325960667
11	32.35590857522421	22.372109833647542	23.873342720108955	21.398638871019283
12	19.973877040681764	27.666195467275827	31.174374939820908	21.1855525522215
13	30.78964738191292	21.194483478484145	27.638286322705063	20.37758281689787
14	20.868086032729057	22.605430282259135	27.717548293286036	28.808935391725775
15	23.28808795846003	29.302229522014034	25.52779681026387	21.881885709262068
16	22.300243786377827	28.36420317299065	26.18938308231383	23.146169958317692
17	20.229524804949968	27.412640888850436	27.535580670684652	24.822253635514947
18	21.162527507950617	26.652395790742816	30.83011564154053	21.354961059766037
19	20.91469430416223	25.29922091622931	31.671436804626218	22.114647974982244
20	24.252767540548497	24.568280419920992	30.653590302130446	20.52536173740007
21	23.875435905951765	25.273823594669913	29.395306519157533	21.45543398022079
22	22.468675473862387	24.932215665123756	30.387197517202498	22.211911343811355
23	21.63475023408795	26.550527413059527	31.185259506203504	20.62946284664902
24	20.818407755393093	25.68352983696873	29.824409616933035	23.673652790705138
25	21.718617213523096	26.322230610470676	30.838069747743198	21.121082428263033
26	22.717345951987898	25.6476665861953	29.862505599272133	21.77248186254467
27	21.63544796270222	26.903717637602547	29.360140996998375	22.100693402696862
28	21.62233066475396	26.271017330183323	29.58327460784163	22.523377397221086
29	20.651929708028486	26.104260191373	30.588980632449125	22.654829468149387
30	20.332788639861793	26.481452280246888	31.72809236810487	21.457666711786448
31	21.446642599681	25.65785342396363	31.1813522259636	21.714151750391775
32	22.16893126117238	25.89494160709227	29.497872625455095	22.438254506280256
33	22.804143391602977	25.74297631490446	29.53694542785416	21.915934865638402
34	21.64451843468772	27.6267040277082	29.562203203690707	21.16657433391338
35	20.63825422718881	28.20847014628578	29.7154244073842	21.43785121914121
36	21.067078233518604	25.48663082202199	31.343783447365446	22.10250749709396
37	21.237603106845974	25.607756509459108	30.874212089962338	22.280428293732584
38	21.26160497117683	25.182560691923513	31.743302851895937	21.81253148500372
39	21.974544069237005	24.986498951313894	31.638643559755568	21.40031341969353
40	20.64913879357141	25.41336931752373	31.102090255382624	22.83540163352223
41	21.172156162827534	25.27312586605564	31.01347872137045	22.541239249746376
42	19.441091470825874	26.670397188990957	31.137674414710354	22.750836925472818
43	19.88680050962098	26.483964103258252	30.73675955295132	22.892475834169446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	59.0
1	90.0
2	121.0
3	189.5
4	258.0
5	258.0
6	525.0
7	792.0
8	958.0
9	1124.0
10	1785.0
11	2446.0
12	2446.0
13	4700.5
14	6955.0
15	11147.0
16	15339.0
17	14341.0
18	13343.0
19	13343.0
20	14810.5
21	16278.0
22	13493.0
23	10708.0
24	11954.0
25	13200.0
26	13200.0
27	14715.0
28	16230.0
29	17625.0
30	19020.0
31	20776.5
32	22533.0
33	22533.0
34	24995.0
35	27457.0
36	29867.0
37	32277.0
38	34155.5
39	36034.0
40	36034.0
41	37914.0
42	39794.0
43	44596.0
44	49398.0
45	58707.0
46	68016.0
47	68016.0
48	68795.5
49	69575.0
50	63774.0
51	57973.0
52	54421.0
53	50869.0
54	50869.0
55	45178.0
56	39487.0
57	34215.0
58	28943.0
59	26023.5
60	23104.0
61	23104.0
62	20479.0
63	17854.0
64	15483.5
65	13113.0
66	10906.5
67	8700.0
68	8700.0
69	7329.0
70	5958.0
71	4952.5
72	3947.0
73	3184.5
74	2422.0
75	2422.0
76	1873.5
77	1325.0
78	1085.0
79	845.0
80	644.0
81	443.0
82	443.0
83	353.5
84	264.0
85	211.5
86	159.0
87	130.0
88	101.0
89	101.0
90	97.5
91	94.0
92	59.0
93	24.0
94	21.5
95	19.0
96	19.0
97	12.0
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	716611.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.81137497448202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.71549490394888	50.66950231848908
2	9.073173018968216	10.853579072917222
3	2.424416439019821	4.350230421855389
4	1.1208147284936552	2.681498800114251
5	0.5966995043718942	1.7844708901537474
6	0.3910233002963449	1.4032584742670502
7	0.27205514530349467	1.139039461663916
8	0.20654997431681674	0.9883230371866206
9	0.1515165893058702	0.8156173984045197
>10	0.937957004464493	10.433582553611174
>50	0.06846985007564431	2.8112019450676
>100	0.034351634586426844	4.318612403175557
>500	0.003972638013284998	1.7056128925889935
>1k	0.0028042150682011744	3.1199451764624695
>5k	7.010537670502936E-4	2.925525154042467
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7679	1.0715716057944966	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7405	1.0333360777325495	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5847	0.8159238415262954	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3382	0.47194363469162487	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2842	0.3965889443505612	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2158	0.3011396699185472	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2042	0.28495236606750385	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2026	0.28271963450184273	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1980	0.2763005312505669	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1692	0.23611136306866629	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1444	0.2015040238009185	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1440	0.2009458409095032	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1210	0.16885032465312422	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1100	0.15350029513920385	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1006	0.1403829971909446	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	973	0.1357779883367685	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	910	0.12698660779697774	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	875	0.12210250749709395	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	851	0.11875341014860223	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	833	0.11624158713723345	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	783	0.10926430099454237	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	772	0.10772929804315032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.7909144570764335E-4	0.0
11	0.0	0.0	0.0	8.3727433712293E-4	0.0
12	0.0	0.0	0.0	0.0022327315656611468	0.0
13	0.0	0.0	0.0	0.0027909144570764334	0.0
14	0.0	0.0	0.0	0.0032095516256378984	0.0
15	0.0	0.0	0.0	0.005163191745591402	0.0
16	0.0	0.0	0.0	0.0073959233112525485	0.0
17	0.0	0.0	0.0	0.01339638939396688	0.0
18	0.0	0.0	0.0	0.015210483791066562	0.0
19	0.0	0.0	0.0	0.01660594101960478	0.0
20	0.0	0.0	0.0	0.023164589993734396	0.0
21	0.0	0.0	0.0	0.02944414752215637	0.0
22	0.0	0.0	0.0	0.05302737468445223	0.0
23	0.0	0.0	0.0	0.07870378768955542	0.0
24	0.0	0.0	0.0	0.11442749274013377	0.0
25	0.0	0.0	0.0	0.12475387623131658	0.0
26	0.0	0.0	0.0	0.1384293570709911	0.0
27	0.0	0.0	0.0	0.18657263145555958	0.0
28	0.0	0.0	0.0	0.28885964630741084	0.0
29	0.0	0.0	0.0	0.4256144547041561	0.0
30	0.0	0.0	0.0	0.7023336231232845	0.0
31	0.0	0.0	0.0	1.0565006677262838	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1655	0.0	30.293053	1
TTCCGCC	35	8.8661216E-4	26.428572	31
ATTGGAC	195	0.0	21.820513	3
TATACTC	70	5.0974995E-6	21.142859	5
TGCGGCG	45	0.0038244224	20.555557	36
GTATTGG	215	0.0	19.790697	1
TTGGACC	375	0.0	19.733335	4
GTATTAG	110	3.8455255E-8	18.5	1
GGACCCT	390	0.0	18.5	6
GTATCAA	2785	0.0	18.001797	2
GACCCTC	395	0.0	17.797468	7
TGCGCAG	75	2.065898E-4	17.266666	10
GACGGAC	65	0.0015791494	17.076923	7
ACTGGTC	65	0.0015791494	17.076923	8
TATTGGA	265	0.0	16.754717	2
TGGACCC	410	0.0	16.695122	5
GCTTCGG	80	3.3803433E-4	16.1875	33
TTTTTAC	300	0.0	16.033333	1
CGCGCGT	70	0.002591216	15.857143	7
ACGGACC	70	0.002591216	15.857143	8
>>END_MODULE