FastQCFastQC Report
Wed 25 May 2016
SRR2088129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670065
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80211.1970480475774739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76981.1488437688880928No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63210.9433413176333638No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG53250.7946990217366973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30850.4604030952221053No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29060.4336892689515196No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT27500.4104079454978249No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT24080.35936812100318627No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA24050.3589204032444614No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA21010.31355167036033815No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG19690.2938520889764426No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT18680.27877892443270424No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT18350.2738540290867304No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA14950.2231126830979084No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA14470.21594919895831002No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12480.18625058762955832No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT11490.17147590159163661No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC11430.17058046607418684No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA11260.16804339877474572No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.14685142486176714No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT9590.14312044353905964No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG8950.13356913135292844No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG8720.13013662853603755No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC8530.12730108273077984No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA8370.12491325468424704No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.11924216307373164No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC7760.11580966025684075No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.11521270324520755No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC7700.11491422473939095No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA7600.11342183221030797No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG7080.10566139105907636No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA6930.10342280226545185No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA6920.10327356301254356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCT303.59789E-430.83333415
GGTATCA18900.029.365081
TTGACGG358.865518E-426.42857233
ATTGACG358.865518E-426.42857232
GTATTGG2150.024.9534911
GCATTAT400.00193035123.1250021
ATTGGAC2600.020.6346153
CATGTGG450.003824167420.5555571
TCTATAC759.256315E-619.7333343
TATTGGA2750.018.8363632
ACAACTA1205.162292E-918.58
AGTATGG1002.8704744E-718.52
CGATGCG500.007031894318.533
CGCACAA500.007031894318.533
ATACTCT852.7203727E-517.4117646
TTGGACC4150.017.3855424
GTATCAA31950.017.3708932
TATACTC752.0656452E-417.2666665
TGTTAGT650.001579004217.0769238
ATACTGC1005.870432E-616.6500026
Produced by FastQC (version 0.11.5)