##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088129_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	670065
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.451002514681413	31.0	31.0	34.0	30.0	34.0
2	31.638991739607352	31.0	31.0	34.0	30.0	34.0
3	31.733900442494384	31.0	31.0	34.0	30.0	34.0
4	35.47875952332983	37.0	35.0	37.0	33.0	37.0
5	35.37012528635282	37.0	35.0	37.0	33.0	37.0
6	35.3814958250319	37.0	35.0	37.0	33.0	37.0
7	35.23085820032385	37.0	35.0	37.0	32.0	37.0
8	35.26292523859625	37.0	35.0	37.0	32.0	37.0
9	36.75524016326774	39.0	36.0	39.0	32.0	39.0
10	36.625849730996244	39.0	35.0	39.0	32.0	39.0
11	36.71184138852201	39.0	35.0	39.0	32.0	39.0
12	36.58898315835031	39.0	35.0	39.0	32.0	39.0
13	36.59709878892346	39.0	35.0	39.0	32.0	39.0
14	37.55234193697626	39.0	36.0	41.0	32.0	41.0
15	37.563500555916214	39.0	36.0	41.0	32.0	41.0
16	37.437460544872515	39.0	36.0	41.0	32.0	41.0
17	37.48653339601382	39.0	36.0	41.0	32.0	41.0
18	37.474509189406994	39.0	36.0	41.0	32.0	41.0
19	37.53198271809451	39.0	36.0	41.0	32.0	41.0
20	37.44353607485841	39.0	36.0	41.0	32.0	41.0
21	37.33902681083178	39.0	36.0	41.0	31.0	41.0
22	37.28064292270153	39.0	36.0	41.0	31.0	41.0
23	37.043222672427305	39.0	36.0	40.0	31.0	41.0
24	36.9160797833046	39.0	36.0	40.0	31.0	41.0
25	36.8140807235119	39.0	35.0	40.0	30.0	41.0
26	36.8799026960071	39.0	36.0	40.0	31.0	41.0
27	36.78295389253282	39.0	35.0	40.0	30.0	41.0
28	36.65949124338684	39.0	35.0	40.0	30.0	41.0
29	36.561090341981746	39.0	35.0	40.0	30.0	41.0
30	36.386893808809596	38.0	35.0	40.0	30.0	41.0
31	36.20952295672808	38.0	35.0	40.0	30.0	41.0
32	36.07624633431085	38.0	35.0	40.0	30.0	41.0
33	35.866462208890184	38.0	35.0	40.0	29.0	41.0
34	35.712778611030274	38.0	34.0	40.0	28.0	41.0
35	35.4905912113004	38.0	34.0	40.0	27.0	41.0
36	35.38417019244402	38.0	34.0	40.0	26.0	41.0
37	35.236108437241164	38.0	34.0	40.0	26.0	41.0
38	35.102355741607155	38.0	34.0	40.0	25.0	41.0
39	35.0169595487005	38.0	34.0	40.0	25.0	41.0
40	34.78348070709558	38.0	33.0	40.0	24.0	41.0
41	34.651139814794085	38.0	33.0	40.0	23.0	41.0
42	34.4268839590189	38.0	33.0	40.0	22.0	41.0
43	34.09529075537448	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	1.0
15	6.0
16	7.0
17	26.0
18	53.0
19	121.0
20	265.0
21	535.0
22	905.0
23	1625.0
24	2571.0
25	4132.0
26	6113.0
27	8653.0
28	11790.0
29	15675.0
30	19819.0
31	24447.0
32	30085.0
33	36734.0
34	45608.0
35	54969.0
36	69626.0
37	93533.0
38	119451.0
39	123313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.508756613164394	22.906732928894957	14.01923693969988	22.565273518240765
2	17.89751740502787	22.65377239521539	36.12649519076507	23.322215008991666
3	19.843746502205008	25.755710266914406	32.10927298097946	22.291270249901128
4	13.209166274913628	15.390894913180064	35.16658831605889	36.23335049584742
5	11.230253781349571	40.87857148187116	34.30562706603091	13.58554767074836
6	31.89287606426242	36.10246767104684	15.364181086909479	16.640475177781262
7	25.17218478804295	35.025706461313455	20.91215031377553	18.889958436868064
8	28.325460962742422	33.80089991269504	19.830016490937446	18.043622633625095
9	25.51200256691515	13.747770738659682	23.145963451306965	37.594263243118206
10	16.48974353234388	27.80297433831046	33.070075291203096	22.637206838142568
11	31.873027243625618	21.596113809854266	24.80968264272869	21.721176303791424
12	19.91582906135972	27.40555020781566	31.645735861446276	21.032884869378343
13	31.26980218337027	20.79275891144889	28.440822905240537	19.496615999940303
14	20.79887772081813	22.18784744763568	27.896995067642692	29.116279763903503
15	23.170289449531015	28.748554244737452	26.56279614664249	21.518360159089045
16	22.11472021371061	28.56872094498295	26.194324431211896	23.122234410094546
17	19.683314305328587	26.789639811063104	27.449725026676518	26.07732085693179
18	20.88200398468805	26.713229313574054	31.604396588390678	20.800370113347213
19	20.210875064359428	24.514039682717346	32.65190690455403	22.623178348369187
20	25.191138173162305	23.699491840343846	31.178020042831665	19.931349943662184
21	24.118406423257447	24.745360524725214	29.833971331139512	21.302261720877823
22	22.201129741144516	24.193026049711595	31.272040772163894	22.33380343697999
23	21.223164916836428	26.336847917739327	32.445359778528946	19.9946273868953
24	19.96492877556655	25.238745494840053	30.611060121033034	24.185265608560364
25	21.092729809794573	26.584435838314192	31.5849954855126	20.737838866378635
26	22.934342190682994	25.29859043525628	30.07409728906897	21.692970084991757
27	21.240924387932512	27.051405460664263	29.695029586681887	22.012640564721334
28	21.123025378134958	26.14955265533941	29.986643086864706	22.74077887966093
29	20.0989456246782	25.960914239663317	31.209808003701134	22.730332131957347
30	19.36871796019789	26.217158036906866	33.240208039518556	21.17391596337669
31	20.91916455866222	25.743771126681743	31.96346623088805	21.37359808376799
32	22.209934857066106	25.6531829001664	29.818151970331236	22.31873027243626
33	23.12402528112944	25.749292979039346	29.47266309984852	21.654018639982688
34	21.128696469745474	28.726317596054113	29.52131509629663	20.623670837903784
35	19.77718579540791	29.531761844000208	29.75039734951087	20.940655011081013
36	20.741868326207157	25.566176415720864	32.203293710311684	21.488661547760294
37	20.946923059703163	26.127912963667704	31.111907053793285	21.813256922835844
38	21.050345861968616	25.462305895696684	32.24597613664346	21.24137210569124
39	22.25888533202003	24.83520255497601	31.956601225254268	20.949310887749697
40	20.502488564542247	25.586323714863486	30.95535507749248	22.95583264310179
41	21.385537224000657	25.261728339787933	30.852678471491572	22.500055964719838
42	19.089192839500647	27.09125234119078	31.254281301067806	22.565273518240765
43	19.78942341414639	26.66472655637886	30.874467402416183	22.67138262705857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40.0
1	57.0
2	74.0
3	201.5
4	329.0
5	329.0
6	594.0
7	859.0
8	995.5
9	1132.0
10	1876.0
11	2620.0
12	2620.0
13	4989.5
14	7359.0
15	11746.0
16	16133.0
17	15139.5
18	14146.0
19	14146.0
20	15233.5
21	16321.0
22	13157.5
23	9994.0
24	11025.5
25	12057.0
26	12057.0
27	13305.0
28	14553.0
29	15462.5
30	16372.0
31	17620.0
32	18868.0
33	18868.0
34	21026.0
35	23184.0
36	25566.0
37	27948.0
38	29712.5
39	31477.0
40	31477.0
41	32875.5
42	34274.0
43	39422.5
44	44571.0
45	57515.5
46	70460.0
47	70460.0
48	70938.0
49	71416.0
50	64785.0
51	58154.0
52	54591.0
53	51028.0
54	51028.0
55	44419.5
56	37811.0
57	31139.5
58	24468.0
59	21479.0
60	18490.0
61	18490.0
62	16360.0
63	14230.0
64	12532.5
65	10835.0
66	9180.0
67	7525.0
68	7525.0
69	6364.0
70	5203.0
71	4296.0
72	3389.0
73	2708.5
74	2028.0
75	2028.0
76	1601.5
77	1175.0
78	902.0
79	629.0
80	515.0
81	401.0
82	401.0
83	304.0
84	207.0
85	165.0
86	123.0
87	100.0
88	77.0
89	77.0
90	73.5
91	70.0
92	44.5
93	19.0
94	14.5
95	10.0
96	10.0
97	7.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	670065.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.09198130100591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.22563990643565	47.243830186970456
2	9.28850733174073	10.420215591325146
3	2.611800403705033	4.395031782197472
4	1.103886649811825	2.4767675727869976
5	0.6204919702582827	1.7402311996575954
6	0.39988576456194463	1.3458230897008232
7	0.2692539953331954	1.0572093050015507
8	0.21215217617315132	0.9520028719097691
9	0.1539895866037468	0.7773822911094295
>10	0.9799524177437824	10.189608804577384
>50	0.07575577641121266	2.9558008974240093
>100	0.04721396252818586	5.461982671926253
>500	0.0064018932241545826	2.560346458894848
>1k	0.004001183265096614	4.329337586963676
>5k	0.001066982204025764	4.094429689554553
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8021	1.1970480475774739	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7698	1.1488437688880928	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6321	0.9433413176333638	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5325	0.7946990217366973	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3085	0.4604030952221053	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2906	0.4336892689515196	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2750	0.4104079454978249	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2408	0.35936812100318627	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2405	0.3589204032444614	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2101	0.31355167036033815	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1969	0.2938520889764426	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1868	0.27877892443270424	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1835	0.2738540290867304	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1495	0.2231126830979084	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1447	0.21594919895831002	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1248	0.18625058762955832	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1149	0.17147590159163661	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1143	0.17058046607418684	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1126	0.16804339877474572	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	984	0.14685142486176714	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	959	0.14312044353905964	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	895	0.13356913135292844	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	872	0.13013662853603755	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	853	0.12730108273077984	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	837	0.12491325468424704	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	799	0.11924216307373164	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	776	0.11580966025684075	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	772	0.11521270324520755	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	770	0.11491422473939095	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	760	0.11342183221030797	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	708	0.10566139105907636	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	693	0.10342280226545185	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	692	0.10327356301254356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.4923925290829995E-4	0.0
11	0.0	0.0	0.0	5.969570116331998E-4	0.0
12	0.0	0.0	0.0	5.969570116331998E-4	0.0
13	0.0	0.0	0.0	7.461962645414997E-4	0.0
14	0.0	0.0	0.0	0.0013431532761746996	0.0
15	0.0	0.0	0.0	0.0017908710348995994	0.0
16	0.0	0.0	0.0	0.0037309813227074987	0.0
17	0.0	0.0	0.0	0.006268048622148597	0.0
18	0.0	0.0	0.0	0.006715766380873497	0.0
19	0.0	0.0	0.0	0.006865005633781797	0.0
20	0.0	0.0	0.0	0.008954355174497997	0.0
21	0.0	0.0	0.0	0.011939140232663995	0.0
22	0.0	0.0	0.0	0.023729041212419692	0.0
23	0.0	0.0	0.0	0.039100684261974585	0.0
24	0.0	0.0	0.0	0.06044189742786148	0.0
25	0.0	0.0	0.0	0.06775462082036818	0.0
26	0.0	0.0	0.0	0.07805212927104087	0.0
27	0.0	0.0	0.0	0.12282390514353085	0.0
28	0.0	0.0	0.0	0.19654809608023102	0.0
29	0.0	0.0	0.0	0.2862408870781193	0.0
30	0.0	0.0	0.0	0.4750285420071187	0.0
31	0.0	0.0	0.0	0.689634587689254	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTCT	30	3.59789E-4	30.833334	15
GGTATCA	1890	0.0	29.36508	1
TTGACGG	35	8.865518E-4	26.428572	33
ATTGACG	35	8.865518E-4	26.428572	32
GTATTGG	215	0.0	24.953491	1
GCATTAT	40	0.001930351	23.125002	1
ATTGGAC	260	0.0	20.634615	3
CATGTGG	45	0.0038241674	20.555557	1
TCTATAC	75	9.256315E-6	19.733334	3
TATTGGA	275	0.0	18.836363	2
ACAACTA	120	5.162292E-9	18.5	8
AGTATGG	100	2.8704744E-7	18.5	2
CGATGCG	50	0.0070318943	18.5	33
CGCACAA	50	0.0070318943	18.5	33
ATACTCT	85	2.7203727E-5	17.411764	6
TTGGACC	415	0.0	17.385542	4
GTATCAA	3195	0.0	17.370893	2
TATACTC	75	2.0656452E-4	17.266666	5
TGTTAGT	65	0.0015790042	17.076923	8
ATACTGC	100	5.870432E-6	16.650002	6
>>END_MODULE