FastQCFastQC Report
Wed 25 May 2016
SRR2088128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636956
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80301.2606836264985337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75131.1795163245184912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63510.9970861409579312No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG49220.7727378343245059No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31420.49328368050540383No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25370.39830066755003485No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT23800.37365218319632754No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT21880.34350881379561543No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA20620.32372722762639805No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA20400.3202732998825665No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG17950.2818091045535327No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT17340.27223230490018147No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT15840.24868279755587513No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA14350.2252902869271975No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA13250.2080206482080395No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12200.19153599306702504No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT11350.17819127223858477No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC10160.1595086630787684No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA9860.15479876160990713No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.1480479028378726No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC9010.14145404078146684No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG8310.13046427068745722No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT8220.12905130024679884No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG8200.1287373068155414No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.1268533462279969No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC7660.12025948417159113No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA7440.11680555642775953No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.11319463196829924No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC6410.1006348947180025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.028.5669521
TCGTATA358.865037E-426.4285713
CGCAAGA502.7002697E-422.22
CGGACCA502.7002697E-422.29
ACTTCGA603.72293E-521.5833327
GCGCAAG603.72293E-521.5833321
TATAGTG705.0960316E-621.1428575
AAGACGG909.461655E-820.5555555
CGTCTGT1052.2546374E-819.38095334
GTATTGG3300.018.5000021
TAGACAG609.230654E-418.55
ATTACTC902.1491524E-618.53
GTCTAAC609.230654E-418.51
GACGGAC609.230654E-418.57
CCGAATT801.6148266E-518.514
ATAGTGT701.2181148E-418.56
TAAGCTA500.007031522718.533
TAGTAGT500.007031522718.54
CGAAGCT609.230654E-418.511
ATTGGAC3350.018.2238833
Produced by FastQC (version 0.11.5)