##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	636956
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.700217911441293	31.0	31.0	34.0	30.0	34.0
2	31.871297232461895	31.0	31.0	34.0	30.0	34.0
3	31.975109740704223	31.0	31.0	34.0	30.0	34.0
4	35.66366279617431	37.0	35.0	37.0	35.0	37.0
5	35.579997676448606	37.0	35.0	37.0	33.0	37.0
6	35.60811107831624	37.0	35.0	37.0	33.0	37.0
7	35.46374474845986	37.0	35.0	37.0	33.0	37.0
8	35.519203838255706	37.0	35.0	37.0	33.0	37.0
9	37.04142672335295	39.0	37.0	39.0	33.0	39.0
10	36.92020013941308	39.0	37.0	39.0	33.0	39.0
11	36.99033057228443	39.0	37.0	39.0	33.0	39.0
12	36.86734719509668	39.0	37.0	39.0	33.0	39.0
13	36.86790139350285	39.0	37.0	39.0	32.0	39.0
14	37.8769789436005	40.0	37.0	41.0	33.0	41.0
15	37.883398539302554	40.0	37.0	41.0	33.0	41.0
16	37.77731114865077	40.0	37.0	41.0	32.0	41.0
17	37.807901958691026	40.0	37.0	41.0	32.0	41.0
18	37.79789969793832	40.0	37.0	41.0	32.0	41.0
19	37.85159414465049	40.0	37.0	41.0	32.0	41.0
20	37.75912778904665	40.0	37.0	41.0	32.0	41.0
21	37.675877140650215	40.0	37.0	41.0	32.0	41.0
22	37.57471159703339	39.0	36.0	41.0	32.0	41.0
23	37.345956392592264	39.0	36.0	41.0	31.0	41.0
24	37.19374179692161	39.0	36.0	41.0	31.0	41.0
25	37.100515577214125	39.0	36.0	41.0	31.0	41.0
26	37.244462725839774	39.0	36.0	41.0	31.0	41.0
27	37.160800746048395	39.0	36.0	41.0	31.0	41.0
28	37.07290770477081	39.0	36.0	41.0	31.0	41.0
29	36.98541971501956	39.0	36.0	41.0	31.0	41.0
30	36.78364753609355	39.0	35.0	41.0	30.0	41.0
31	36.593017414075696	39.0	35.0	40.0	30.0	41.0
32	36.43939769780016	38.0	35.0	40.0	30.0	41.0
33	36.21908577672555	38.0	35.0	40.0	30.0	41.0
34	36.08607658927775	38.0	35.0	40.0	30.0	41.0
35	35.860244663681634	38.0	35.0	40.0	29.0	41.0
36	35.76630881881951	38.0	35.0	40.0	28.0	41.0
37	35.60566506948675	38.0	35.0	40.0	27.0	41.0
38	35.474057862709515	38.0	34.0	40.0	26.0	41.0
39	35.35083742048116	38.0	34.0	40.0	25.0	41.0
40	35.129340174203556	38.0	34.0	40.0	24.0	41.0
41	34.968754513655576	38.0	34.0	40.0	24.0	41.0
42	34.74010763694824	38.0	33.0	40.0	23.0	41.0
43	34.416595494822246	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	3.0
16	9.0
17	16.0
18	38.0
19	90.0
20	178.0
21	364.0
22	754.0
23	1369.0
24	2174.0
25	3296.0
26	4989.0
27	7076.0
28	9593.0
29	12753.0
30	16161.0
31	20169.0
32	25327.0
33	31813.0
34	39765.0
35	49554.0
36	64616.0
37	90685.0
38	119182.0
39	136979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.98571957874641	22.642537318119306	13.860769032711836	22.510974070422446
2	17.727912132078195	22.813977731585855	36.436111756542054	23.021998379793896
3	19.711408637331306	25.88781642688035	32.25717317993708	22.143601755851268
4	12.878126589591746	15.556490558217522	35.45472528714699	36.11065756504374
5	11.051783796682974	41.04993123543855	34.42325686546637	13.475028102412098
6	31.733903126746586	36.507388265437484	15.420060412336174	16.338648195479752
7	25.25684662676857	35.10760554889192	20.83095221647963	18.804595607859884
8	28.348111957497853	33.823058421617816	19.739668046144473	18.089161574739858
9	25.63772065888382	13.861240022858723	23.07208033207945	37.42895898617801
10	16.34116014292981	28.12454863444257	32.93524199473747	22.599049227890152
11	31.880224065712547	21.852058854928753	24.604054283184396	21.663662796174304
12	20.077524978177458	27.55370857641658	31.718517448614975	20.65024899679099
13	31.321158761358713	20.898617800915606	28.26113577703954	19.51908766068614
14	20.746173990040127	22.349267453324874	28.02925162805594	28.875306928579054
15	23.080558154723406	29.00922512701034	26.519885204001532	21.39033151426472
16	21.94358794014029	28.893518547591984	26.190820088043758	22.972073424223964
17	19.588480208994028	27.13703929313799	27.52937408549413	25.745106412373854
18	20.93080212761949	26.790704538461057	31.4539779827806	20.824515351138853
19	20.092910656309073	24.717562908583954	32.722825438491824	22.466700996615153
20	25.0043959080376	23.939487185928073	31.289602421517344	19.766514484516982
21	23.897726059570836	24.945365142961208	30.064871042897785	21.092037754570175
22	22.275008006832497	24.332763958577985	31.353029094631342	22.039198939958176
23	21.1714780926783	26.465407343678372	32.361575995830165	20.001538567813164
24	19.93701291768976	25.43613687601655	30.789410885524276	23.83743932076941
25	21.119669176520826	26.64249963890755	31.615056613015657	20.62277457155596
26	22.805813902373163	25.347590728401965	30.382475398614663	21.464119970610216
27	21.2349047657923	26.957592047174373	29.92530096270386	21.88220222432947
28	21.080576994329277	26.095052091510247	30.1333216109119	22.691049303248576
29	19.975791106450053	26.16146170222119	31.385527414766486	22.477219776562272
30	19.398985173230177	26.53495688870189	33.214382155125314	20.85167578294262
31	20.751511878371502	25.92298369118118	32.05339144305101	21.272112987396305
32	22.10576554738475	25.896451246239927	30.01133516286839	21.98644804350693
33	23.045547887138202	25.734273638995468	29.668297339219663	21.551881134646663
34	20.986064971520797	28.6644603394897	29.88322584291537	20.46624884607414
35	19.77703326446411	29.46247464503043	30.146038344877823	20.614453745627642
36	20.668617612519547	25.7163760134138	32.27978070698761	21.335225667079044
37	20.829225252607717	26.231011247244705	31.383015467316422	21.556748032831152
38	21.06801725707898	25.563932202538325	32.238961560924146	21.129088979458547
39	22.01659141290764	25.084778226439504	32.17349393050697	20.72513643014588
40	20.32809173632088	25.784983578143546	31.14893336431402	22.73799132122156
41	21.329102795169526	25.392020798924886	31.025376949114225	22.253499456791364
42	19.013558236361693	27.018349776122687	31.676913318973366	22.291178668542255
43	19.78770904112686	26.820062924283622	30.85456452251019	22.537663512079327
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30.0
1	59.0
2	88.0
3	202.5
4	317.0
5	317.0
6	535.5
7	754.0
8	915.5
9	1077.0
10	1712.0
11	2347.0
12	2347.0
13	4699.0
14	7051.0
15	11356.0
16	15661.0
17	14594.5
18	13528.0
19	13528.0
20	14725.0
21	15922.0
22	12923.5
23	9925.0
24	11291.0
25	12657.0
26	12657.0
27	13850.0
28	15043.0
29	16097.5
30	17152.0
31	18329.5
32	19507.0
33	19507.0
34	21286.5
35	23066.0
36	24844.0
37	26622.0
38	28202.5
39	29783.0
40	29783.0
41	31144.0
42	32505.0
43	37186.5
44	41868.0
45	53244.0
46	64620.0
47	64620.0
48	65416.5
49	66213.0
50	59933.5
51	53654.0
52	50466.5
53	47279.0
54	47279.0
55	40996.0
56	34713.0
57	29315.5
58	23918.0
59	20964.5
60	18011.0
61	18011.0
62	15827.0
63	13643.0
64	11908.5
65	10174.0
66	8567.5
67	6961.0
68	6961.0
69	5883.0
70	4805.0
71	4043.0
72	3281.0
73	2657.0
74	2033.0
75	2033.0
76	1581.0
77	1129.0
78	883.0
79	637.0
80	534.0
81	431.0
82	431.0
83	326.5
84	222.0
85	175.0
86	128.0
87	110.0
88	92.0
89	92.0
90	78.5
91	65.0
92	44.0
93	23.0
94	19.5
95	16.0
96	16.0
97	9.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	636956.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.52027288325275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.58440786393612	48.65318222001956
2	9.288697564522758	10.685768372827086
3	2.5421218221013078	4.3867062272921675
4	1.0210939452888133	2.349344094897988
5	0.5506143837291282	1.583574480277175
6	0.36774378808936287	1.2691633825212736
7	0.25875359249901825	1.0418504075045858
8	0.2089848316331997	0.9616691635201825
9	0.14062934400736596	0.7280134418426865
>10	0.9124764717558015	9.87725469139947
>50	0.07206529060692698	2.864280655137397
>100	0.042584020237026066	5.310112970827193
>500	0.004913540796578667	2.060045600912036
>1k	0.0040946173304822215	4.7913331944992965
>5k	8.189234660964445E-4	3.43770109652196
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8030	1.2606836264985337	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7513	1.1795163245184912	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6351	0.9970861409579312	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4922	0.7727378343245059	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3142	0.49328368050540383	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2537	0.39830066755003485	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2380	0.37365218319632754	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2188	0.34350881379561543	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2062	0.32372722762639805	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2040	0.3202732998825665	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1795	0.2818091045535327	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1734	0.27223230490018147	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1584	0.24868279755587513	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1435	0.2252902869271975	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1325	0.2080206482080395	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1220	0.19153599306702504	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1135	0.17819127223858477	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1016	0.1595086630787684	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	986	0.15479876160990713	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	943	0.1480479028378726	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	901	0.14145404078146684	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	831	0.13046427068745722	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	822	0.12905130024679884	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	820	0.1287373068155414	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	808	0.1268533462279969	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	766	0.12025948417159113	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	744	0.11680555642775953	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	721	0.11319463196829924	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	641	0.1006348947180025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.139934312574181E-4	0.0
11	0.0	0.0	0.0	3.139934312574181E-4	0.0
12	0.0	0.0	0.0	9.419802937722543E-4	0.0
13	0.0	0.0	0.0	0.0012559737250296724	0.0
14	0.0	0.0	0.0	0.0015699671562870905	0.0
15	0.0	0.0	0.0	0.002040957303173218	0.0
16	0.0	0.0	0.0	0.004081914606346436	0.0
17	0.0	0.0	0.0	0.005965875193890944	0.0
18	0.0	0.0	0.0	0.006750858772034489	0.0
19	0.0	0.0	0.0	0.007535842350178034	0.0
20	0.0	0.0	0.0	0.00989079308460867	0.0
21	0.0	0.0	0.0	0.015856668278499613	0.0
22	0.0	0.0	0.0	0.025276471216222157	0.0
23	0.0	0.0	0.0	0.03956317233843468	0.0
24	0.0	0.0	0.0	0.0573038012044788	0.0
25	0.0	0.0	0.0	0.06499664027028555	0.0
26	0.0	0.0	0.0	0.0726894793360923	0.0
27	0.0	0.0	0.0	0.11806153015278921	0.0
28	0.0	0.0	0.0	0.18494213101061927	0.0
29	0.0	0.0	0.0	0.28180910455353275	0.0
30	0.0	0.0	0.0	0.45089456728565236	0.0
31	0.0	0.0	0.0	0.6427445537839348	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1755	0.0	28.566952	1
TCGTATA	35	8.865037E-4	26.42857	13
CGCAAGA	50	2.7002697E-4	22.2	2
CGGACCA	50	2.7002697E-4	22.2	9
ACTTCGA	60	3.72293E-5	21.583332	7
GCGCAAG	60	3.72293E-5	21.583332	1
TATAGTG	70	5.0960316E-6	21.142857	5
AAGACGG	90	9.461655E-8	20.555555	5
CGTCTGT	105	2.2546374E-8	19.380953	34
GTATTGG	330	0.0	18.500002	1
TAGACAG	60	9.230654E-4	18.5	5
ATTACTC	90	2.1491524E-6	18.5	3
GTCTAAC	60	9.230654E-4	18.5	1
GACGGAC	60	9.230654E-4	18.5	7
CCGAATT	80	1.6148266E-5	18.5	14
ATAGTGT	70	1.2181148E-4	18.5	6
TAAGCTA	50	0.0070315227	18.5	33
TAGTAGT	50	0.0070315227	18.5	4
CGAAGCT	60	9.230654E-4	18.5	11
ATTGGAC	335	0.0	18.223883	3
>>END_MODULE