FastQCFastQC Report
Wed 25 May 2016
SRR2088127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551431
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75521.3695276471580307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72411.3131289318155854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61431.1140106377769838No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG49560.8987525184474576No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29360.5324328882489378No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25280.4584435768029001No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT24160.4381327854255564No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT22550.4089360228206249No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA20740.3761123331840248No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA19800.3590657761351828No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG18270.3313197843429187No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT17480.3169934225678281No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT16830.3052059097149054No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA13800.25025796518512744No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA13460.24409218923129092No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA11120.20165714296076936No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT10750.19494732795218261No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC9790.17753807820017373No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA9680.17554326833275605No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8900.16139825290924886No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT8530.15468843790066208No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG8190.14852266194682562No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG8010.14525842761832394No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC7470.13546572463281897No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA7430.1347403392264853No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.13238283665590073No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC7290.1322014903043173No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.13183879760115044No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA6860.12440359718623002No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG6710.12168340191247862No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC6460.11714974312289297No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA6390.115880318661809No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC6120.11098396716905652No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC6070.11007723541113937No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA5700.10336742040255263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.030.1162781
GTATTGG3250.026.1846141
ATTGGAC3150.025.253973
AATGCGT551.9003319E-523.54545435
TGCGTTA551.9003319E-523.54545437
TCCGGCG400.001929920323.12517
CGTTTAG400.001929920323.12526
TTGGGTA400.001929920323.1254
CGTCTGT1054.0017767E-1122.90476434
TGGACCC4750.022.5894745
TAACACC502.6996317E-422.24
AAATGCG753.7334758E-722.234
ATGCGTT603.721762E-521.58333436
GGACCCT4850.021.3608256
TATTGGA3950.021.0759492
TTGGACC5050.020.881194
GTATTAG450.003823322520.5555551
ACCGCCG450.003823322520.55555512
GGGGTTA450.003823322520.5555556
GTAAGTG450.003823322520.5555553
Produced by FastQC (version 0.11.5)