##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551431
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.636284503410216	31.0	31.0	34.0	30.0	34.0
2	31.80321382004276	31.0	31.0	34.0	30.0	34.0
3	31.90428539563427	31.0	31.0	34.0	30.0	34.0
4	35.604218841523235	37.0	35.0	37.0	33.0	37.0
5	35.52176246892177	37.0	35.0	37.0	33.0	37.0
6	35.543106934503136	37.0	35.0	37.0	33.0	37.0
7	35.39237003360348	37.0	35.0	37.0	33.0	37.0
8	35.456856796226546	37.0	35.0	37.0	33.0	37.0
9	36.95021679956332	39.0	37.0	39.0	33.0	39.0
10	36.840402516361976	39.0	37.0	39.0	32.0	39.0
11	36.90867760426962	39.0	37.0	39.0	33.0	39.0
12	36.798272131962115	39.0	37.0	39.0	32.0	39.0
13	36.792632260427865	39.0	37.0	39.0	32.0	39.0
14	37.766184708512945	40.0	37.0	41.0	32.0	41.0
15	37.77382483030515	40.0	37.0	41.0	32.0	41.0
16	37.64798859694141	39.0	36.0	41.0	32.0	41.0
17	37.71783233079025	39.0	37.0	41.0	32.0	41.0
18	37.70781475832879	39.0	37.0	41.0	32.0	41.0
19	37.76465958569612	40.0	37.0	41.0	32.0	41.0
20	37.676853495722945	39.0	37.0	41.0	32.0	41.0
21	37.59528027985369	39.0	37.0	41.0	32.0	41.0
22	37.515999281868446	39.0	36.0	41.0	32.0	41.0
23	37.265199091092086	39.0	36.0	41.0	31.0	41.0
24	37.11669637724393	39.0	36.0	41.0	31.0	41.0
25	36.991369727128145	39.0	36.0	41.0	31.0	41.0
26	37.13247350983169	39.0	36.0	41.0	31.0	41.0
27	37.048441237434965	39.0	36.0	41.0	31.0	41.0
28	36.9571206551681	39.0	35.0	41.0	31.0	41.0
29	36.87115341719998	39.0	35.0	41.0	31.0	41.0
30	36.66118335748262	39.0	35.0	40.0	30.0	41.0
31	36.44793092880161	38.0	35.0	40.0	30.0	41.0
32	36.28958292152599	38.0	35.0	40.0	30.0	41.0
33	36.0615815940707	38.0	35.0	40.0	30.0	41.0
34	35.906546784638515	38.0	35.0	40.0	29.0	41.0
35	35.673518536317324	38.0	35.0	40.0	27.0	41.0
36	35.58530804398012	38.0	35.0	40.0	27.0	41.0
37	35.41615904800419	38.0	34.0	40.0	26.0	41.0
38	35.26994311164951	38.0	34.0	40.0	25.0	41.0
39	35.13086315422963	38.0	34.0	40.0	25.0	41.0
40	34.89002794547278	38.0	34.0	40.0	24.0	41.0
41	34.71770538834414	38.0	33.0	40.0	23.0	41.0
42	34.46305340106015	38.0	33.0	40.0	22.0	41.0
43	34.13889861106829	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	4.0
14	3.0
15	3.0
16	7.0
17	19.0
18	37.0
19	69.0
20	210.0
21	331.0
22	710.0
23	1173.0
24	1940.0
25	3150.0
26	4574.0
27	6569.0
28	9034.0
29	11728.0
30	14878.0
31	18397.0
32	23054.0
33	28466.0
34	35429.0
35	43608.0
36	56773.0
37	78424.0
38	100427.0
39	112413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.701737842087226	22.98909564387929	14.055793018528156	22.25337349550533
2	17.728419330795695	22.72124708259057	36.61056415036514	22.939769436248596
3	19.680431459239685	25.951206950642963	32.47496060250512	21.893400987612228
4	12.898440602722733	15.417704118919684	35.28655443745455	36.39730084090303
5	10.718113417635207	41.40572437893408	34.348268414361904	13.527893789068804
6	31.526156491020636	36.22901142663361	15.295295331600872	16.94953675074488
7	24.889061369418837	35.475335989452894	20.717369897593716	18.91823274353455
8	28.68518454711469	33.72770119924342	19.738462291746384	17.848651961895502
9	25.46574276745413	13.674784333851377	23.477098675990288	37.3823742227042
10	16.47259584608047	28.118658544768067	32.75006301785717	22.658682591294287
11	31.43747812509634	21.65347976446736	24.985718974812805	21.923323135623495
12	19.910559979399054	27.650059572276497	31.708772267065143	20.730608181259306
13	31.492426069626116	20.96853459453676	28.757541741396476	18.781497594440648
14	20.666955611853524	22.395367688795154	28.247233108040714	28.69044359131061
15	22.98111640440962	28.9410642491989	27.061772007739865	21.016047338651617
16	21.89285694855748	28.884121494801708	26.347811421555917	22.8752101350849
17	19.24556290814263	27.080632028304542	27.531459058340936	26.14234600521189
18	20.573743587139642	26.703612963362595	32.134936193286194	20.587707256211566
19	19.716700729556372	24.529451554228906	33.309153819788875	22.444693896425843
20	25.12934528526688	23.466761933950032	31.90897863921325	19.494914141569843
21	23.736423958754585	24.824683414606724	30.636833982855517	20.802058643783177
22	22.048814810919225	23.87207102973899	32.01434086948321	22.064773289858568
23	20.816022312855097	26.354158543861335	33.29446476531062	19.535354377972947
24	19.548411315286955	25.150200115698972	31.2934891219391	24.007899447074973
25	20.797887677696757	26.544572213023933	32.404235525387584	20.25330458389173
26	22.794148315927107	25.059708286258843	30.703569440238216	21.442573957575835
27	20.922291274882987	27.234051041744117	30.1851002210612	21.658557462311695
28	20.56032395712247	26.185869129591914	30.634476480284935	22.619330433000684
29	19.548411315286955	25.998538348406235	31.96301985198511	22.490030484321704
30	18.86110864278577	26.297941174870477	34.12938336800071	20.711566814343048
31	20.349780842934113	25.811207567220556	32.731747036347244	21.10726455349808
32	22.009643998977207	25.788176580569466	30.384218515099803	21.817960905353527
33	23.012670669585134	25.76296943769937	29.996681361766026	21.227678530949476
34	20.734053761939393	29.089586911145727	30.078287219978566	20.098072106936314
35	19.285459105490986	29.91181126922498	30.442068001254917	20.360661624029117
36	20.260558437955066	25.556053250542675	33.07159009921459	21.111798212287667
37	20.55524625927813	26.116413476935463	31.95993696400819	21.368403299778212
38	20.754183206965152	25.29509585061413	32.97638326463329	20.97433767778743
39	21.915343896153825	24.90429446295185	32.8001146108942	20.380247030000127
40	20.05853860229113	25.772218101630123	31.51491301722246	22.654330278856285
41	21.309465735513598	25.347867638924903	31.30981029358161	22.032856331979886
42	18.560799084563616	27.25853279920788	31.833719903306125	22.346948212922378
43	19.544603041903702	26.90653953078445	31.278256028406094	22.270601398905757
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33.0
1	50.5
2	68.0
3	170.0
4	272.0
5	272.0
6	519.5
7	767.0
8	853.5
9	940.0
10	1580.5
11	2221.0
12	2221.0
13	4436.0
14	6651.0
15	10609.5
16	14568.0
17	13754.5
18	12941.0
19	12941.0
20	14058.0
21	15175.0
22	12150.5
23	9126.0
24	10165.5
25	11205.0
26	11205.0
27	11981.5
28	12758.0
29	13482.5
30	14207.0
31	15173.0
32	16139.0
33	16139.0
34	17491.5
35	18844.0
36	20712.0
37	22580.0
38	23468.0
39	24356.0
40	24356.0
41	25638.5
42	26921.0
43	31497.0
44	36073.0
45	47880.5
46	59688.0
47	59688.0
48	59654.5
49	59621.0
50	53512.5
51	47404.0
52	44451.5
53	41499.0
54	41499.0
55	35649.0
56	29799.0
57	24428.0
58	19057.0
59	16466.5
60	13876.0
61	13876.0
62	12251.5
63	10627.0
64	9293.5
65	7960.0
66	6769.0
67	5578.0
68	5578.0
69	4765.5
70	3953.0
71	3268.0
72	2583.0
73	2143.0
74	1703.0
75	1703.0
76	1315.0
77	927.0
78	731.5
79	536.0
80	437.0
81	338.0
82	338.0
83	252.5
84	167.0
85	131.0
86	95.0
87	83.5
88	72.0
89	72.0
90	68.5
91	65.0
92	40.0
93	15.0
94	15.0
95	15.0
96	15.0
97	9.5
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	551431.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.98223907788547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.49098837934524	46.45503723000003
2	9.246985179837678	10.168398998149978
3	2.3944515620309663	3.9495692473200865
4	1.0654611692961533	2.34325762953778
5	0.6332672908553876	1.7409226793007875
6	0.37375004474643964	1.2329768589371481
7	0.27356675454343055	1.0528918891447674
8	0.20622767608684806	0.9071087512867042
9	0.15968380877737898	0.7901796015958676
>10	1.01205537218906	10.324468024763702
>50	0.07938954617590704	3.1177119648699967
>100	0.051933994485300436	6.145941095377845
>500	0.0066157954758344515	2.631922158830871
>1k	0.004631056833084116	5.331865801139231
>5k	9.923693213751677E-4	3.8077480697452226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7552	1.3695276471580307	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7241	1.3131289318155854	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6143	1.1140106377769838	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4956	0.8987525184474576	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2936	0.5324328882489378	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2528	0.4584435768029001	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2416	0.4381327854255564	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2255	0.4089360228206249	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2074	0.3761123331840248	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1980	0.3590657761351828	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1827	0.3313197843429187	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1748	0.3169934225678281	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1683	0.3052059097149054	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1380	0.25025796518512744	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1346	0.24409218923129092	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1112	0.20165714296076936	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1075	0.19494732795218261	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	979	0.17753807820017373	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	968	0.17554326833275605	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	890	0.16139825290924886	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	853	0.15468843790066208	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	819	0.14852266194682562	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	801	0.14525842761832394	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	747	0.13546572463281897	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	743	0.1347403392264853	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	730	0.13238283665590073	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	729	0.1322014903043173	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	727	0.13183879760115044	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	686	0.12440359718623002	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	671	0.12168340191247862	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	646	0.11714974312289297	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	639	0.115880318661809	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	612	0.11098396716905652	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	607	0.11007723541113937	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	570	0.10336742040255263	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	3.626927031668513E-4	0.0
12	0.0	0.0	0.0	0.001088078109500554	0.0
13	0.0	0.0	0.0	0.0012694244610839797	0.0
14	0.0	0.0	0.0	0.0023575025705845336	0.0
15	0.0	0.0	0.0	0.003264234328501662	0.0
16	0.0	0.0	0.0	0.004715005141169067	0.0
17	0.0	0.0	0.0	0.007797893118087304	0.0
18	0.0	0.0	0.0	0.008885971227587858	0.0
19	0.0	0.0	0.0	0.011424820149755818	0.0
20	0.0	0.0	0.0	0.013963669071923776	0.0
21	0.0	0.0	0.0	0.018134635158342568	0.0
22	0.0	0.0	0.0	0.031372918823932644	0.0
23	0.0	0.0	0.0	0.05059563209177576	0.0
24	0.0	0.0	0.0	0.07217584793020342	0.0
25	0.0	0.0	0.0	0.08106181915779127	0.0
26	0.0	0.0	0.0	0.08922240497904543	0.0
27	0.0	0.0	0.0	0.1383672662581538	0.0
28	0.0	0.0	0.0	0.2127192704073583	0.0
29	0.0	0.0	0.0	0.3059312951212391	0.0
30	0.0	0.0	0.0	0.4932620763069178	0.0
31	0.0	0.0	0.0	0.7074321175269436	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1720	0.0	30.116278	1
GTATTGG	325	0.0	26.184614	1
ATTGGAC	315	0.0	25.25397	3
AATGCGT	55	1.9003319E-5	23.545454	35
TGCGTTA	55	1.9003319E-5	23.545454	37
TCCGGCG	40	0.0019299203	23.125	17
CGTTTAG	40	0.0019299203	23.125	26
TTGGGTA	40	0.0019299203	23.125	4
CGTCTGT	105	4.0017767E-11	22.904764	34
TGGACCC	475	0.0	22.589474	5
TAACACC	50	2.6996317E-4	22.2	4
AAATGCG	75	3.7334758E-7	22.2	34
ATGCGTT	60	3.721762E-5	21.583334	36
GGACCCT	485	0.0	21.360825	6
TATTGGA	395	0.0	21.075949	2
TTGGACC	505	0.0	20.88119	4
GTATTAG	45	0.0038233225	20.555555	1
ACCGCCG	45	0.0038233225	20.555555	12
GGGGTTA	45	0.0038233225	20.555555	6
GTAAGTG	45	0.0038233225	20.555555	3
>>END_MODULE