FastQCFastQC Report
Wed 25 May 2016
SRR2088126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639706
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81751.2779307994610025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79351.2404135649814134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64861.0139032618108943No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG47550.7433102081268582No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32090.5016366893541722No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25620.40049647806961325No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT23810.37220223039958983No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT22950.3587585547110704No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA20660.32296086014512915No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA19070.2981056923024014No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG17930.2802850059245966No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT16820.2629332849777867No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT16260.2541792635992159No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA14320.2238528323948814No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA13220.20665743325840308No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT12090.18899306869092988No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA11010.17211031317511483No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC10090.15772870662460567No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA9530.1489746852460349No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.1461608926600657No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT8410.13146664248889334No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG8090.12646434455828146No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC8030.12552641369629175No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG8020.12537009188596013No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.1222436556793277No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7680.12005515033468499No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC7540.1178666449900423No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA7480.11692871412805257No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG7110.11114480714578259No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA6670.10426664749119126No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC6460.1009838894742272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.028.9233061
GGCGTCG358.8650786E-426.428578
GTATACG701.9201434E-723.7857131
GTAAGAC551.9009494E-523.5454543
CGCTTAA400.001930256423.12529
GGGCGTC652.6797588E-622.769237
GACCGTC502.7002883E-422.19999922
CGTCTGT1450.021.68965534
ACGGGCG603.7229613E-521.5833345
CGGGCGT603.7229613E-521.5833346
TATACGG705.096088E-621.1428572
GTAGACC450.00382398120.5555573
GTATACT450.00382398120.5555574
GAACCGC450.00382398120.5555576
AGTACGG450.00382398120.55555736
CGTGGTT555.1396684E-420.18181836
AGACCGT555.1396684E-420.18181821
GTATTGG2400.020.0416681
TCGACAA656.896018E-519.92307713
CGAATTA609.230712E-418.515
Produced by FastQC (version 0.11.5)