##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088126_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639706
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.527101512257193	31.0	31.0	34.0	30.0	34.0
2	31.70266341100443	31.0	31.0	34.0	30.0	34.0
3	31.790858613175427	31.0	31.0	34.0	30.0	34.0
4	35.522186754540364	37.0	35.0	37.0	33.0	37.0
5	35.42562520908042	37.0	35.0	37.0	33.0	37.0
6	35.439948976561105	37.0	35.0	37.0	33.0	37.0
7	35.297338152213676	37.0	35.0	37.0	32.0	37.0
8	35.333106145635654	37.0	35.0	37.0	32.0	37.0
9	36.828421180980015	39.0	37.0	39.0	32.0	39.0
10	36.694289251624966	39.0	35.0	39.0	32.0	39.0
11	36.78513567169919	39.0	37.0	39.0	32.0	39.0
12	36.671366221357935	39.0	35.0	39.0	32.0	39.0
13	36.6803047024727	39.0	35.0	39.0	32.0	39.0
14	37.63047399899329	39.0	36.0	41.0	32.0	41.0
15	37.65071142055882	39.0	36.0	41.0	32.0	41.0
16	37.517150065811485	39.0	36.0	41.0	32.0	41.0
17	37.58161405395604	39.0	36.0	41.0	32.0	41.0
18	37.56922711370536	39.0	36.0	41.0	32.0	41.0
19	37.61001147402088	39.0	37.0	41.0	32.0	41.0
20	37.53696854492532	39.0	36.0	41.0	32.0	41.0
21	37.45118538828775	39.0	36.0	41.0	32.0	41.0
22	37.379547792267076	39.0	36.0	41.0	32.0	41.0
23	37.15015022525973	39.0	36.0	41.0	31.0	41.0
24	37.009141699468195	39.0	36.0	40.0	31.0	41.0
25	36.90677436197253	39.0	36.0	40.0	31.0	41.0
26	36.971371223655865	39.0	36.0	40.0	31.0	41.0
27	36.88365123978828	39.0	35.0	40.0	31.0	41.0
28	36.77679121346368	39.0	35.0	40.0	30.0	41.0
29	36.67257927860611	39.0	35.0	40.0	30.0	41.0
30	36.47447577480905	38.0	35.0	40.0	30.0	41.0
31	36.30649079420859	38.0	35.0	40.0	30.0	41.0
32	36.15563243114806	38.0	35.0	40.0	30.0	41.0
33	35.945528101971846	38.0	35.0	40.0	29.0	41.0
34	35.80413033487258	38.0	35.0	40.0	29.0	41.0
35	35.58530481189796	38.0	34.0	40.0	27.0	41.0
36	35.49038151901029	38.0	34.0	40.0	27.0	41.0
37	35.33204941019781	38.0	34.0	40.0	26.0	41.0
38	35.17517422065762	38.0	34.0	40.0	25.0	41.0
39	35.062267666709396	38.0	34.0	40.0	25.0	41.0
40	34.8457166260751	38.0	33.0	40.0	24.0	41.0
41	34.69591968810673	38.0	33.0	40.0	23.0	41.0
42	34.46786648866823	38.0	33.0	40.0	22.0	41.0
43	34.12192944884056	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	5.0
16	7.0
17	19.0
18	56.0
19	109.0
20	211.0
21	451.0
22	865.0
23	1412.0
24	2354.0
25	3842.0
26	5589.0
27	7925.0
28	10791.0
29	14180.0
30	18185.0
31	22568.0
32	28312.0
33	34558.0
34	42531.0
35	52431.0
36	66569.0
37	89221.0
38	114744.0
39	122770.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.379643148571375	23.185338264765377	14.044576727434166	22.39044185922908
2	17.771913972981338	22.81938890677905	36.32496803218979	23.08372908804982
3	19.673256152044846	25.938790631946553	32.498053793461374	21.88989942254723
4	12.935942448562306	15.707840789362612	35.54992449656561	35.80629226550947
5	10.839979615635933	41.17328897962501	34.68186948379412	13.304861920944935
6	31.211212650811465	36.61134958871732	15.61607988669795	16.561357873773265
7	24.678993162484016	35.30825097779292	21.209586903984018	18.803168955739043
8	28.165594820120493	33.95294088221777	19.93681472426396	17.94464957339778
9	25.416206820007943	13.765854939612884	23.487039358705406	37.33089888167377
10	16.380806182840242	28.22327756813286	33.16851803797369	22.227398211053202
11	31.541676957852516	21.84331552306841	24.93364139151423	21.68136612756485
12	19.785182568242284	27.864987978852785	31.698780377235792	20.651049075669135
13	30.829631111791976	21.197706446398815	28.666449900423007	19.3062125413862
14	20.641200801618243	22.618984345933914	28.318164907004157	28.42164994544369
15	23.062938287275717	29.105245222023868	26.713208880329404	21.118607610371015
16	21.813145413674405	28.619240713702858	26.801374381356435	22.766239491266298
17	19.344511384917446	27.297539807349004	27.801521323858147	25.556427483875403
18	20.52614794921417	26.77964564972034	31.99923089669317	20.69497550437232
19	19.7689250999678	24.99460689754356	33.091138741859545	22.1453292606291
20	24.57503915861349	24.077310514517606	31.718164281716916	19.629486045151992
21	23.485007175171095	25.16656088890834	30.621097816809595	20.727334119110967
22	21.907720108925037	24.563002379217952	31.808830931709252	21.720446580147758
23	20.893035238062485	26.514054893966915	32.921373255839406	19.6715366121312
24	19.64855730601245	25.68508033377833	31.12851841314604	23.537843947063184
25	20.754377792298335	26.860932991092785	32.023617099104904	20.36107211750398
26	22.355738417335463	25.705402169121438	30.726146073352446	21.21271334019065
27	20.860363979703177	27.251424873301172	30.297199025802477	21.591012121193174
28	20.48065830240767	26.58361809956449	30.61750241517197	22.318221182855876
29	19.626984896186688	26.403691695872794	31.879332068168814	22.089991339771707
30	18.81161033349695	26.807939897390366	33.69985587129087	20.68059389782181
31	20.37639165491648	26.17327334744398	32.55245378345678	20.897881214182764
32	21.740768415490866	26.17686874908161	30.373171425623646	21.70919140980388
33	22.48110850922142	26.200317020631353	30.06803125185632	21.2505432182909
34	20.634635285584316	28.85763147445858	30.160104798141646	20.34762844181546
35	19.293706796559672	29.77367728300188	30.418192106999154	20.514423813439297
36	20.28697557940679	25.86188030126339	32.71956179870128	21.13158232062854
37	20.62259850618878	26.27644574226285	31.68783785051258	21.41311790103579
38	20.68246975954579	25.719471132051286	32.67313422103278	20.924924887370135
39	21.7162258912688	25.3833792398383	32.435994034759716	20.46440083413318
40	19.977927360381177	26.135130825723067	31.431782725189382	22.455159088706374
41	20.94212028650661	25.620832069732035	31.394265490709795	22.042782153051558
42	18.663260935492243	27.43525932225116	31.6612631427562	22.240216599500396
43	19.40922861439474	26.927057116863057	31.37238043726337	22.291333831478834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	71.0
2	90.0
3	219.5
4	349.0
5	349.0
6	624.0
7	899.0
8	1043.0
9	1187.0
10	1906.0
11	2625.0
12	2625.0
13	5093.0
14	7561.0
15	11872.5
16	16184.0
17	15255.0
18	14326.0
19	14326.0
20	15752.0
21	17178.0
22	13880.5
23	10583.0
24	11785.5
25	12988.0
26	12988.0
27	14278.0
28	15568.0
29	16522.0
30	17476.0
31	18909.5
32	20343.0
33	20343.0
34	22116.5
35	23890.0
36	26017.0
37	28144.0
38	29266.5
39	30389.0
40	30389.0
41	31974.0
42	33559.0
43	38013.0
44	42467.0
45	53810.0
46	65153.0
47	65153.0
48	65892.0
49	66631.0
50	60158.0
51	53685.0
52	50145.5
53	46606.0
54	46606.0
55	40223.0
56	33840.0
57	28050.5
58	22261.0
59	19499.0
60	16737.0
61	16737.0
62	14591.5
63	12446.0
64	10888.0
65	9330.0
66	7911.0
67	6492.0
68	6492.0
69	5330.5
70	4169.0
71	3428.5
72	2688.0
73	2193.5
74	1699.0
75	1699.0
76	1287.0
77	875.0
78	701.5
79	528.0
80	414.0
81	300.0
82	300.0
83	217.5
84	135.0
85	109.0
86	83.0
87	84.5
88	86.0
89	86.0
90	76.0
91	66.0
92	44.5
93	23.0
94	17.5
95	12.0
96	12.0
97	7.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	639706.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.75890621379859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.98454594604455	45.98901874867912
2	9.382475303754095	10.275481704231039
3	2.595724664466014	4.264171303750149
4	1.0746853649629375	2.3539438043738965
5	0.6138395594373782	1.680659143277542
6	0.40736753647232693	1.3384200434540596
7	0.29370301920707836	1.1257999258428921
8	0.21691145962027214	0.9502267419235714
9	0.16936725916946388	0.8346929274493912
>10	1.1128115678958768	11.516441128314549
>50	0.08330131716455828	3.161221508717403
>100	0.05438916817122103	5.992792470706109
>500	0.005725175596970076	2.217886549345204
>1k	0.0042938816977275565	4.757271813278507
>5k	8.587763395455114E-4	3.5419721866565994
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8175	1.2779307994610025	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7935	1.2404135649814134	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6486	1.0139032618108943	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4755	0.7433102081268582	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3209	0.5016366893541722	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2562	0.40049647806961325	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2381	0.37220223039958983	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2295	0.3587585547110704	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2066	0.32296086014512915	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1907	0.2981056923024014	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1793	0.2802850059245966	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1682	0.2629332849777867	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1626	0.2541792635992159	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1432	0.2238528323948814	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1322	0.20665743325840308	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1209	0.18899306869092988	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1101	0.17211031317511483	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1009	0.15772870662460567	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	953	0.1489746852460349	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	935	0.1461608926600657	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	841	0.13146664248889334	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	809	0.12646434455828146	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	803	0.12552641369629175	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	802	0.12537009188596013	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	782	0.1222436556793277	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	768	0.12005515033468499	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	754	0.1178666449900423	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	748	0.11692871412805257	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	711	0.11114480714578259	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	667	0.10426664749119126	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	646	0.1009838894742272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.563218103316211E-4	0.0
12	0.0	0.0	0.0	6.252872413264844E-4	0.0
13	0.0	0.0	0.0	6.252872413264844E-4	0.0
14	0.0	0.0	0.0	0.001563218103316211	0.0
15	1.563218103316211E-4	0.0	0.0	0.0021885053446426953	0.0
16	1.563218103316211E-4	0.0	0.0	0.0037517234479589063	0.0
17	1.563218103316211E-4	0.0	0.0	0.00719080327525457	0.0
18	1.563218103316211E-4	0.0	0.0	0.007972412326912676	0.0
19	1.563218103316211E-4	0.0	0.0	0.008910343188902402	0.0
20	1.563218103316211E-4	0.0	0.0	0.012036779395534825	0.0
21	1.563218103316211E-4	0.0	0.0	0.01735172094680994	0.0
22	1.563218103316211E-4	0.0	0.0	0.027981604049360176	0.0
23	1.563218103316211E-4	0.0	0.0	0.04564596861683336	0.0
24	1.563218103316211E-4	0.0	0.0	0.06753102206326031	0.0
25	1.563218103316211E-4	0.0	0.0	0.07440918171785164	0.0
26	1.563218103316211E-4	0.0	0.0	0.08347584671708566	0.0
27	1.563218103316211E-4	0.0	0.0	0.12630802274794983	0.0
28	1.563218103316211E-4	0.0	0.0	0.20024823903480662	0.0
29	1.563218103316211E-4	0.0	0.0	0.2921654635097998	0.0
30	1.563218103316211E-4	0.0	0.0	0.47193554539116406	0.0
31	1.563218103316211E-4	0.0	0.0	0.6696826354606648	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1695	0.0	28.923306	1
GGCGTCG	35	8.8650786E-4	26.42857	8
GTATACG	70	1.9201434E-7	23.785713	1
GTAAGAC	55	1.9009494E-5	23.545454	3
CGCTTAA	40	0.0019302564	23.125	29
GGGCGTC	65	2.6797588E-6	22.76923	7
GACCGTC	50	2.7002883E-4	22.199999	22
CGTCTGT	145	0.0	21.689655	34
ACGGGCG	60	3.7229613E-5	21.583334	5
CGGGCGT	60	3.7229613E-5	21.583334	6
TATACGG	70	5.096088E-6	21.142857	2
GTAGACC	45	0.003823981	20.555557	3
GTATACT	45	0.003823981	20.555557	4
GAACCGC	45	0.003823981	20.555557	6
AGTACGG	45	0.003823981	20.555557	36
CGTGGTT	55	5.1396684E-4	20.181818	36
AGACCGT	55	5.1396684E-4	20.181818	21
GTATTGG	240	0.0	20.041668	1
TCGACAA	65	6.896018E-5	19.923077	13
CGAATTA	60	9.230712E-4	18.5	15
>>END_MODULE