FastQCFastQC Report
Wed 25 May 2016
SRR2088125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855097
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT408131.4294785781358743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT401121.4049259972603383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT312691.095199217399619No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG209980.7354566237154114No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150800.5281782020015432No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT121400.4252044676590672No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT110780.38800783300882596No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA107410.3762043811471204No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA96100.33659101599700464No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA93140.3262235924033404No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG88350.3094465792230527No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT83220.2914787133326819No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT79000.2766981296957687No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA66010.23120055115465427No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA61920.2168752935539493No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT57220.2004135060910365No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA51230.17943348334574974No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47560.1665792790927944No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA44260.1550210027890471No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC41460.14521398047071607No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41400.1450038299924661No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC40290.14111604614484202No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG39980.14003026867388393No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT39530.1384541400870093No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38860.1361074597465515No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG36730.12864711776867827No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA36160.12665068822530373No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35350.12381365676892939No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC32220.11285080682022362No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG30850.10805237090018306No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC30590.10714171882776663No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC28870.10111740511793468No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC28870.10111740511793468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92950.029.6358261
ATTGGAC11250.020.5555553
GTATTGG12200.019.7131161
TTGGACC16600.019.280124
TATTGGA13200.018.0795462
GTATCAA153100.017.9804042
CTGTGCG2100.017.619059
TGGACCC18300.017.2868845
ACGTTTA2300.016.89130426
ATCACGT2200.016.81818223
GGACCCT18450.016.6449876
CACGGTA803.384766E-416.187532
GCGCTTA803.384766E-416.187518
CGTCTGT8500.016.10588334
TCACGTT2300.016.08695624
GACCCTC19150.015.9399477
CATACGC700.002593757815.85714212
TGCGACG957.065541E-515.57894822
TTTTTAC19850.015.3778341
CTAGCGG1101.4532909E-515.13636429
Produced by FastQC (version 0.11.5)