##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088125_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2855097
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.466901825051828	31.0	31.0	34.0	30.0	34.0
2	31.639349205998954	31.0	31.0	34.0	30.0	34.0
3	31.713318321584172	31.0	31.0	34.0	30.0	34.0
4	35.45060535596514	37.0	35.0	37.0	33.0	37.0
5	35.343033529158554	37.0	35.0	37.0	33.0	37.0
6	35.37156075607939	37.0	35.0	37.0	33.0	37.0
7	35.22881219096934	37.0	35.0	37.0	32.0	37.0
8	35.26744450363683	37.0	35.0	37.0	32.0	37.0
9	36.73530076211071	39.0	35.0	39.0	32.0	39.0
10	36.60205274987155	39.0	35.0	39.0	32.0	39.0
11	36.69248680517685	39.0	35.0	39.0	32.0	39.0
12	36.57909766288151	38.0	35.0	39.0	32.0	39.0
13	36.59996560537173	39.0	35.0	39.0	32.0	39.0
14	37.529226502637215	39.0	36.0	41.0	32.0	41.0
15	37.54997185734845	39.0	36.0	41.0	32.0	41.0
16	37.40870380235768	39.0	36.0	41.0	32.0	41.0
17	37.48178082916272	39.0	36.0	41.0	32.0	41.0
18	37.48005619423788	39.0	36.0	41.0	32.0	41.0
19	37.53236965329024	39.0	36.0	41.0	32.0	41.0
20	37.449598384923526	39.0	36.0	41.0	32.0	41.0
21	37.376054824056766	39.0	36.0	41.0	31.0	41.0
22	37.30604354247859	39.0	36.0	41.0	31.0	41.0
23	37.08559954355316	39.0	36.0	40.0	31.0	41.0
24	36.945243891888786	39.0	36.0	40.0	31.0	41.0
25	36.84925450869095	39.0	35.0	40.0	30.0	41.0
26	36.90594154944648	39.0	36.0	40.0	31.0	41.0
27	36.79222457240507	39.0	35.0	40.0	30.0	41.0
28	36.675673015662866	39.0	35.0	40.0	30.0	41.0
29	36.561458682489594	39.0	35.0	40.0	30.0	41.0
30	36.36965118873369	38.0	35.0	40.0	30.0	41.0
31	36.18356889450691	38.0	35.0	40.0	30.0	41.0
32	36.02642256988116	38.0	35.0	40.0	30.0	41.0
33	35.809294045000925	38.0	35.0	40.0	29.0	41.0
34	35.65504009145749	38.0	34.0	40.0	28.0	41.0
35	35.44480870527341	38.0	34.0	40.0	27.0	41.0
36	35.3298549926675	38.0	34.0	40.0	26.0	41.0
37	35.16633935729679	38.0	34.0	40.0	25.0	41.0
38	35.00658366423277	38.0	34.0	40.0	25.0	41.0
39	34.88880517894839	38.0	33.0	40.0	24.0	41.0
40	34.65852403613607	38.0	33.0	40.0	23.0	41.0
41	34.48863698851563	38.0	33.0	40.0	23.0	41.0
42	34.25490937786002	38.0	33.0	40.0	21.0	41.0
43	33.90375458346949	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	13.0
14	13.0
15	16.0
16	42.0
17	96.0
18	254.0
19	508.0
20	1168.0
21	2168.0
22	4182.0
23	7183.0
24	11655.0
25	18390.0
26	26703.0
27	37667.0
28	51513.0
29	67432.0
30	85250.0
31	105565.0
32	129559.0
33	158251.0
34	193073.0
35	234574.0
36	295856.0
37	396188.0
38	496022.0
39	531751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.31407689476049	23.135501175616803	14.47302841199441	22.077393517628295
2	17.99399459983321	22.93785465082272	36.015273736759205	23.05287701258486
3	19.952772182521294	25.583193845953396	32.52092660949873	21.943107362026577
4	13.234716718906572	15.741916999667612	34.672762431539105	36.35060384988671
5	10.9793817863281	41.17502837907083	34.11162562953203	13.733964205069041
6	31.702950897990505	36.09446544197973	15.080713544933849	17.121870115095913
7	24.692506068970687	35.659979328197956	20.666268081259588	18.98124652157177
8	28.409262452379025	33.90756951515132	19.61975372465454	18.063414307815112
9	25.300471402547792	13.839915071186724	23.502564010960047	37.357049515305434
10	16.69999303000914	28.365551152903034	32.69275965054778	22.241696166540052
11	31.64887217492085	21.795021325019782	24.596011974374253	21.96009452568512
12	19.958621370832585	27.908754063347057	31.442574455438816	20.690050110381538
13	31.444851085619856	20.963911208620935	28.66081257484422	18.93042513091499
14	20.617338044907054	22.68378272261853	28.476195379701636	28.22268385277278
15	23.25017328658186	29.04097478999838	26.719967832966795	20.98888409045297
16	21.920761361172666	28.43910382029052	26.79429105210786	22.845843766428953
17	19.28782104425874	27.267199678329668	27.887493839964105	25.557485437447486
18	20.41419258259877	26.586452229118663	32.31004060457491	20.689314583707663
19	19.819746929789076	24.76858054209717	33.653217386309464	21.75845514180429
20	24.70248821668756	23.557553386102118	32.32552168980599	19.414436707404338
21	23.663294101741553	24.693311645803977	30.88073715183757	20.762657100616895
22	21.912915743318003	24.057606449097875	32.16370582155352	21.865771986030598
23	20.83344278670742	26.414514112830496	33.22153327890436	19.530509821557725
24	19.478287427712612	25.312870280764542	31.493605996573848	23.715236294948998
25	20.73754411846603	26.439416944503115	32.67020349921561	20.15283543781525
26	22.40522826369822	25.243590673101473	31.054811797987945	21.296369265212356
27	20.923772467275192	26.955371393686452	30.578891014911225	21.54196512412713
28	20.63936882004359	26.162963990365302	30.853067338867994	22.34459985072311
29	19.44799073376491	26.090321975050234	32.26643438033804	22.19525291084681
30	18.90531214876412	26.193575910030376	34.23526416090241	20.665847780303086
31	20.40704746633827	25.622597060625264	32.86697439701698	21.103381076019485
32	21.731976181544795	25.867282267467623	30.81506512738446	21.58567642360312
33	22.779471240381675	25.791452969899098	30.29525791943321	21.133817870286016
34	20.730118801567862	28.661688201836927	30.39963265696402	20.208560339631195
35	19.37090053332689	29.39917628017542	30.71097059049132	20.518952596006372
36	20.24792852922335	25.444984881424343	33.175650424486456	21.131436164865853
37	20.327680635719204	25.864830511888037	32.29659097396691	21.510897878425848
38	20.6415053499058	25.262399140904847	33.170046411733125	20.926049097456236
39	21.753761781123373	24.80644265326187	32.928338336665966	20.51145722894879
40	20.048250549806188	25.499133654653416	31.960805534803193	22.491810260737203
41	20.85417763389475	25.237286158753975	31.796713036369695	22.11182317098158
42	18.38049635441458	26.93337564362962	32.244683805839166	22.44144419611663
43	19.177492043177516	26.639199999159395	31.762423483335244	22.420884474327842
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	161.0
1	318.5
2	476.0
3	965.5
4	1455.0
5	1455.0
6	2809.5
7	4164.0
8	4835.5
9	5507.0
10	8821.0
11	12135.0
12	12135.0
13	23911.5
14	35688.0
15	57803.5
16	79919.0
17	74663.5
18	69408.0
19	69408.0
20	75523.0
21	81638.0
22	64240.0
23	46842.0
24	51049.5
25	55257.0
26	55257.0
27	59691.0
28	64125.0
29	67075.0
30	70025.0
31	75723.0
32	81421.0
33	81421.0
34	88797.5
35	96174.0
36	106243.0
37	116312.0
38	123349.5
39	130387.0
40	130387.0
41	138404.0
42	146421.0
43	169098.0
44	191775.0
45	247434.0
46	303093.0
47	303093.0
48	305026.5
49	306960.0
50	275872.5
51	244785.0
52	227170.0
53	209555.0
54	209555.0
55	180639.0
56	151723.0
57	124807.5
58	97892.0
59	85039.5
60	72187.0
61	72187.0
62	63707.0
63	55227.0
64	48267.0
65	41307.0
66	35164.0
67	29021.0
68	29021.0
69	24448.5
70	19876.0
71	16865.0
72	13854.0
73	11210.0
74	8566.0
75	8566.0
76	6701.5
77	4837.0
78	3807.0
79	2777.0
80	2287.0
81	1797.0
82	1797.0
83	1410.0
84	1023.0
85	778.5
86	534.0
87	431.5
88	329.0
89	329.0
90	302.5
91	276.0
92	187.5
93	99.0
94	76.5
95	54.0
96	54.0
97	35.0
98	16.0
99	17.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2855097.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.579781598208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.55444953489328	30.25403279300589
2	12.998467938940506	10.54949980147029
3	4.991506522303253	6.076627335632902
4	2.330219458359269	3.782391867844547
5	1.296478483291813	2.6305406849378867
6	0.8031118120872905	1.955406116006599
7	0.5382850272661481	1.5290442190832008
8	0.36422608450824373	1.1824171969371984
9	0.2736831621687473	0.9995402653123234
>10	1.5776409979442907	12.136131739889395
>50	0.15874343567730248	4.489432023949093
>100	0.0963132370201799	7.273044354363302
>500	0.007961210818664882	2.157436749018593
>1k	0.0074420014174468845	6.210803826437102
>5k	7.788141018258368E-4	2.3744867269747916
>10k+	6.92279201622966E-4	6.399164299136976
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40813	1.4294785781358743	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40112	1.4049259972603383	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31269	1.095199217399619	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	20998	0.7354566237154114	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15080	0.5281782020015432	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	12140	0.4252044676590672	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11078	0.38800783300882596	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10741	0.3762043811471204	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9610	0.33659101599700464	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9314	0.3262235924033404	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8835	0.3094465792230527	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	8322	0.2914787133326819	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7900	0.2766981296957687	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6601	0.23120055115465427	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6192	0.2168752935539493	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5722	0.2004135060910365	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5123	0.17943348334574974	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4756	0.1665792790927944	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4426	0.1550210027890471	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4146	0.14521398047071607	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4140	0.1450038299924661	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4029	0.14111604614484202	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3998	0.14003026867388393	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3953	0.1384541400870093	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3886	0.1361074597465515	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3673	0.12864711776867827	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3616	0.12665068822530373	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3535	0.12381365676892939	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	3222	0.11285080682022362	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3085	0.10805237090018306	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	3059	0.10714171882776663	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2887	0.10111740511793468	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2887	0.10111740511793468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.502507970832514E-5	0.0	0.0	0.0
6	3.502507970832514E-5	3.502507970832514E-5	0.0	0.0	0.0
7	3.502507970832514E-5	3.502507970832514E-5	0.0	0.0	0.0
8	1.0507523912497544E-4	3.502507970832514E-5	0.0	0.0	0.0
9	1.0507523912497544E-4	3.502507970832514E-5	0.0	1.7512539854162574E-4	0.0
10	1.0507523912497544E-4	3.502507970832514E-5	0.0	3.852758767915766E-4	0.0
11	1.0507523912497544E-4	3.502507970832514E-5	0.0	6.654765144581778E-4	0.0
12	1.0507523912497544E-4	3.502507970832514E-5	0.0	0.0010507523912497544	0.0
13	1.0507523912497544E-4	3.502507970832514E-5	0.0	0.0012959279492080304	0.0
14	1.7512539854162574E-4	3.502507970832514E-5	0.0	0.0017862790651245824	0.0
15	1.7512539854162574E-4	3.502507970832514E-5	0.0	0.0024867806592910853	0.0
16	1.7512539854162574E-4	3.502507970832514E-5	0.0	0.0037476835287907906	0.0
17	1.7512539854162574E-4	3.502507970832514E-5	0.0	0.005849188311290299	0.0
18	1.7512539854162574E-4	3.502507970832514E-5	0.0	0.0065496899054568024	0.0
19	1.7512539854162574E-4	3.502507970832514E-5	0.0	0.007425316898164931	0.0
20	2.4517555795827603E-4	3.502507970832514E-5	0.0	0.009701947079206066	0.0
21	2.4517555795827603E-4	3.502507970832514E-5	0.0	0.01334455536887188	0.0
22	2.8020063766660114E-4	3.502507970832514E-5	0.0	0.022240925614786466	0.0
23	2.8020063766660114E-4	3.502507970832514E-5	0.0	0.03421950287503367	0.0
24	2.8020063766660114E-4	3.502507970832514E-5	0.0	0.049700588106113386	0.0
25	2.8020063766660114E-4	3.502507970832514E-5	0.0	0.05625027801157018	0.0
26	2.8020063766660114E-4	3.502507970832514E-5	0.0	0.06504157301835979	0.0
27	2.8020063766660114E-4	3.502507970832514E-5	0.0	0.11940049672568043	0.0
28	2.8020063766660114E-4	7.005015941665029E-5	0.0	0.20997535285140925	0.0
29	2.8020063766660114E-4	7.005015941665029E-5	0.0	0.3201642536138002	0.0
30	3.152257173749263E-4	7.005015941665029E-5	0.0	0.5369694970083329	0.0
31	3.152257173749263E-4	7.005015941665029E-5	0.0	0.8062773348856449	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9295	0.0	29.635826	1
ATTGGAC	1125	0.0	20.555555	3
GTATTGG	1220	0.0	19.713116	1
TTGGACC	1660	0.0	19.28012	4
TATTGGA	1320	0.0	18.079546	2
GTATCAA	15310	0.0	17.980404	2
CTGTGCG	210	0.0	17.61905	9
TGGACCC	1830	0.0	17.286884	5
ACGTTTA	230	0.0	16.891304	26
ATCACGT	220	0.0	16.818182	23
GGACCCT	1845	0.0	16.644987	6
CACGGTA	80	3.384766E-4	16.1875	32
GCGCTTA	80	3.384766E-4	16.1875	18
CGTCTGT	850	0.0	16.105883	34
TCACGTT	230	0.0	16.086956	24
GACCCTC	1915	0.0	15.939947	7
CATACGC	70	0.0025937578	15.857142	12
TGCGACG	95	7.065541E-5	15.578948	22
TTTTTAC	1985	0.0	15.377834	1
CTAGCGG	110	1.4532909E-5	15.136364	29
>>END_MODULE