FastQCFastQC Report
Wed 25 May 2016
SRR2088124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056220
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT298071.449601696316542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT289951.4101117584694245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT231281.1247823676454853No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG158430.7704914843742401No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113810.5534913579286262No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT86720.42174475493867386No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT82520.401318925017751No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA82050.39903317738374294No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA76210.3706315472079836No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA71090.345731487875811No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG63450.3085759305910846No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT58980.2868370116038167No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT58930.286593846961901No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA50380.245012693194308No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA46780.22750483897637414No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT40410.1965256635963078No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA40040.19472624524613125No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35040.17040978105455643No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA32900.16000233438056238No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC30970.1506161792026145No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30680.14920582427950316No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30220.14696870957387828No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT29370.14283491066131057No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC28940.14074369474083512No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA28290.1375825543959304No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27120.1318925017751019No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG27000.1313089066345041No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA24730.1202692318915291No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG24240.11788621840075478No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC22440.10913229129178785No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC22030.10713834122807872No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC21730.10567935337658421No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC21000.10212914960461429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69600.028.3081871
TAATACG652.6845573E-622.769234
GTATTGG8600.022.1569771
ATTGGAC8750.021.1428573
TTGGACC14300.018.4999984
GGACCCT14500.018.117246
TGGACCC16250.017.5323075
GTATCAA113950.017.2255382
TATTGGA11150.016.5919282
ACGTTTA1452.9831426E-916.58620626
CGTCTGT6450.016.34883734
CGATTAG1059.350195E-615.8571421
TTTTTAC13600.015.6433811
CGATCTG2150.015.48837233
TAGGGGT1455.3516487E-815.3103454
TCTATAC1455.3516487E-815.3103453
CGACGAA2550.015.23529410
ACGGACC855.366709E-415.2352938
TCTGTCG1001.0940306E-414.7999998
ACGATCT2301.8189894E-1214.47826132
Produced by FastQC (version 0.11.5)