##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088124_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2056220
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.476939238019277	31.0	31.0	34.0	30.0	34.0
2	31.64030794370252	31.0	31.0	34.0	30.0	34.0
3	31.722572487379754	31.0	31.0	34.0	30.0	34.0
4	35.46030434486582	37.0	35.0	37.0	33.0	37.0
5	35.35598914513039	37.0	35.0	37.0	33.0	37.0
6	35.38729124315491	37.0	35.0	37.0	33.0	37.0
7	35.234568285494746	37.0	35.0	37.0	32.0	37.0
8	35.28107303693184	37.0	35.0	37.0	32.0	37.0
9	36.7456415169583	39.0	35.0	39.0	32.0	39.0
10	36.61985828364669	39.0	35.0	39.0	32.0	39.0
11	36.712848819678825	39.0	35.0	39.0	32.0	39.0
12	36.58562167472352	39.0	35.0	39.0	32.0	39.0
13	36.61346159457646	39.0	35.0	39.0	32.0	39.0
14	37.531029267296304	39.0	36.0	41.0	32.0	41.0
15	37.55072171265721	39.0	36.0	41.0	32.0	41.0
16	37.41552217175205	39.0	36.0	41.0	32.0	41.0
17	37.48471758858488	39.0	36.0	41.0	32.0	41.0
18	37.485140695061816	39.0	36.0	41.0	32.0	41.0
19	37.53710546536849	39.0	36.0	41.0	32.0	41.0
20	37.45646720681639	39.0	36.0	41.0	32.0	41.0
21	37.37478917625546	39.0	36.0	41.0	31.0	41.0
22	37.30575911137913	39.0	36.0	41.0	31.0	41.0
23	37.08313896372956	39.0	36.0	40.0	31.0	41.0
24	36.943574617502016	39.0	36.0	40.0	31.0	41.0
25	36.84267199035123	39.0	35.0	40.0	30.0	41.0
26	36.90325937886024	39.0	36.0	40.0	31.0	41.0
27	36.79192644755911	39.0	35.0	40.0	30.0	41.0
28	36.6832551964284	39.0	35.0	40.0	30.0	41.0
29	36.56809825796851	39.0	35.0	40.0	30.0	41.0
30	36.368825806577114	38.0	35.0	40.0	30.0	41.0
31	36.166838178794094	38.0	35.0	40.0	30.0	41.0
32	36.012629971501106	38.0	35.0	40.0	30.0	41.0
33	35.79072035093521	38.0	34.0	40.0	29.0	41.0
34	35.62990049702853	38.0	34.0	40.0	28.0	41.0
35	35.402080030346944	38.0	34.0	40.0	27.0	41.0
36	35.29146492106876	38.0	34.0	40.0	26.0	41.0
37	35.116918909455215	38.0	34.0	40.0	25.0	41.0
38	34.957442297030475	38.0	33.0	40.0	25.0	41.0
39	34.837105465368495	38.0	33.0	40.0	24.0	41.0
40	34.59318458141639	38.0	33.0	40.0	23.0	41.0
41	34.4249773856883	38.0	33.0	40.0	23.0	41.0
42	34.18040384783729	38.0	33.0	40.0	21.0	41.0
43	33.83107498224898	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	2.0
13	6.0
14	4.0
15	22.0
16	25.0
17	79.0
18	137.0
19	368.0
20	780.0
21	1564.0
22	2985.0
23	5123.0
24	8400.0
25	13334.0
26	19330.0
27	27075.0
28	37178.0
29	48818.0
30	61830.0
31	76181.0
32	92851.0
33	114037.0
34	140008.0
35	168490.0
36	214663.0
37	287810.0
38	359605.0
39	375514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.55830601783856	23.055023295172695	14.266955870480786	22.11971481650796
2	18.112507416521577	22.879458423709526	35.98792930717531	23.020104852593594
3	20.023732869050978	25.513952787153126	32.45693554191672	22.00537880187918
4	13.107255060256199	15.497806654929919	34.58579334896071	36.80914493585317
5	10.903697075215687	41.27928918111875	34.17800624446801	13.639007499197556
6	32.0084913092957	35.871210279055745	15.011428738170041	17.10886967347852
7	24.928995924560603	35.528931729095135	20.45350205717287	19.088570289171393
8	28.713950841835988	33.57301261538162	19.503506434136426	18.209530108645964
9	25.368297166645593	13.741671611014386	23.419235295834103	37.470795926505915
10	16.650017994183504	28.35756874264427	32.70724922430479	22.285164038867435
11	31.504216474890818	21.68133760006225	24.654317145052573	22.16012877999436
12	20.067210707025513	27.886267033683165	31.381418330723363	20.665103928567955
13	31.68245615741506	20.881569092801357	28.471467060917604	18.96450768886598
14	20.720788631566663	22.625740436334635	28.302564900642928	28.35090603145578
15	23.268327319061193	29.036922119228485	26.77782532997442	20.916925231735902
16	21.893377167812783	28.449582243145187	26.677349699934833	22.979690889107196
17	19.270214276682456	27.16552703504489	27.711042592718677	25.853216095553975
18	20.47499781151822	26.42217272470845	32.31434379589733	20.788485667876007
19	19.843499236463025	24.553209286944004	33.55360807695675	22.049683399636226
20	24.906041182363754	23.493011448191343	32.21313867193199	19.387808697512913
21	23.91558296291253	24.504090029277023	30.864498934938865	20.71582807287158
22	21.914046162375623	24.04518971705362	32.050558792347125	21.990205328223634
23	20.835124646195446	26.341587962377567	33.19056326657653	19.632724124850455
24	19.507056637908395	25.314703679567362	31.351995409051558	23.826244273472682
25	20.73513534543969	26.471389248232192	32.57632938109735	20.217146025230765
26	22.536304481038023	25.016437929793504	31.071042981782103	21.376214607386366
27	20.880401902520155	26.861084903366372	30.59862271546819	21.65989047864528
28	20.72399840483995	26.05338922877902	30.77496571378549	22.44764665259554
29	19.557878048068787	26.108636235422278	32.0829483226503	22.250537393858632
30	18.963535030298313	26.31770919454144	34.118917236482474	20.599838538677766
31	20.51954557391719	25.619632140529706	32.785596871930046	21.075225413623055
32	21.85977181430003	25.854966880975773	30.663401776074544	21.621859528649658
33	22.857038643724895	25.73216873680832	30.109424088862085	21.3013685306047
34	20.70580968962465	28.782669169641377	30.368005369075295	20.143515771658674
35	19.335187869002343	29.633696783418117	30.618756747818814	20.412358599760726
36	20.366935444650863	25.523387575259456	33.03950939101847	21.07016758907121
37	20.390376516131543	26.07838655396796	32.1227786909961	21.408458238904398
38	20.716168503370262	25.430255517405726	32.979496357393664	20.87407962183035
39	21.809339467566698	24.907694701928783	32.86457674762428	20.418389082880235
40	20.044304597757048	25.604312768089017	31.81303557012382	22.538347064030116
41	20.94853663518495	25.293159292293627	31.68410967698009	22.074194395541333
42	18.412572584645613	27.042242561593604	32.09233447782825	22.452850375932538
43	19.21837157502602	26.76853644065324	31.621664996936126	22.39142698738462
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	155.0
1	241.0
2	327.0
3	728.0
4	1129.0
5	1129.0
6	2104.5
7	3080.0
8	3539.5
9	3999.0
10	6543.0
11	9087.0
12	9087.0
13	17778.5
14	26470.0
15	42612.5
16	58755.0
17	54928.0
18	51101.0
19	51101.0
20	54575.0
21	58049.0
22	45456.0
23	32863.0
24	36313.5
25	39764.0
26	39764.0
27	43557.0
28	47350.0
29	49844.0
30	52338.0
31	55454.0
32	58570.0
33	58570.0
34	63503.5
35	68437.0
36	74326.5
37	80216.0
38	84629.0
39	89042.0
40	89042.0
41	94063.5
42	99085.0
43	116069.0
44	133053.0
45	173288.5
46	213524.0
47	213524.0
48	216578.0
49	219632.0
50	198318.5
51	177005.0
52	165452.5
53	153900.0
54	153900.0
55	134227.0
56	114554.0
57	94430.5
58	74307.0
59	65209.5
60	56112.0
61	56112.0
62	49224.0
63	42336.0
64	36788.5
65	31241.0
66	26298.0
67	21355.0
68	21355.0
69	18068.0
70	14781.0
71	12386.0
72	9991.0
73	8128.0
74	6265.0
75	6265.0
76	4957.0
77	3649.0
78	2868.5
79	2088.0
80	1635.0
81	1182.0
82	1182.0
83	898.0
84	614.0
85	476.5
86	339.0
87	276.5
88	214.0
89	214.0
90	194.0
91	174.0
92	110.0
93	46.0
94	36.0
95	26.0
96	26.0
97	17.0
98	8.0
99	7.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2056220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.904817492291855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.36085265651494	33.52609299440078
2	12.789358995421694	11.230289450747811
3	4.532464540640835	5.96991085341361
4	2.0325724951834263	3.569588977635224
5	1.099396747046705	2.4134406765352465
6	0.646133405311012	1.7021021541511818
7	0.4066749019563859	1.2498491144363564
8	0.29592915514951773	1.0394172437990157
9	0.21874056781191822	0.8643388237148303
>10	1.3886930454166764	11.696524742227986
>50	0.14025034981690632	4.26576195720545
>100	0.07411176009142868	6.253850147272163
>500	0.00624058090044768	1.9450954795819466
>1k	0.0070206535130036395	5.849132798215634
>5k	0.001002950501857663	3.0840197048041462
>10k+	5.57194723254257E-4	5.340584881858578
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29807	1.449601696316542	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28995	1.4101117584694245	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23128	1.1247823676454853	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15843	0.7704914843742401	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11381	0.5534913579286262	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8672	0.42174475493867386	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8252	0.401318925017751	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8205	0.39903317738374294	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7621	0.3706315472079836	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7109	0.345731487875811	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6345	0.3085759305910846	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5898	0.2868370116038167	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5893	0.286593846961901	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5038	0.245012693194308	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4678	0.22750483897637414	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4041	0.1965256635963078	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4004	0.19472624524613125	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3504	0.17040978105455643	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3290	0.16000233438056238	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3097	0.1506161792026145	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3068	0.14920582427950316	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3022	0.14696870957387828	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2937	0.14283491066131057	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2894	0.14074369474083512	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2829	0.1375825543959304	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2712	0.1318925017751019	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2700	0.1313089066345041	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2473	0.1202692318915291	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2424	0.11788621840075478	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2244	0.10913229129178785	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2203	0.10713834122807872	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2173	0.10567935337658421	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2100	0.10212914960461429	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	4.8632928383149665E-5	0.0	0.0	0.0	0.0
7	4.8632928383149665E-5	0.0	0.0	0.0	0.0
8	1.45898785149449E-4	0.0	0.0	0.0	0.0
9	1.45898785149449E-4	0.0	0.0	1.45898785149449E-4	0.0
10	1.45898785149449E-4	0.0	0.0	2.91797570298898E-4	0.0
11	1.45898785149449E-4	0.0	0.0	5.349622122146463E-4	0.0
12	1.45898785149449E-4	0.0	0.0	0.0010699244244292926	0.0
13	1.45898785149449E-4	0.0	0.0	0.0012158232095787415	0.0
14	1.45898785149449E-4	0.0	0.0	0.0015076207798776396	0.0
15	1.45898785149449E-4	0.0	0.0	0.0022371147056248846	0.0
16	1.45898785149449E-4	0.0	0.0	0.003987900127418272	0.0
17	1.45898785149449E-4	0.0	0.0	0.006030483119510558	0.0
18	1.45898785149449E-4	0.0	0.0	0.007100407543939851	0.0
19	1.45898785149449E-4	0.0	0.0	0.008316230753518592	0.0
20	1.45898785149449E-4	0.0	0.0	0.010796510101059226	0.0
21	1.45898785149449E-4	0.0	0.0	0.015027574870393245	0.0
22	1.45898785149449E-4	0.0	0.0	0.025726819114686172	0.0
23	1.45898785149449E-4	0.0	0.0	0.04021943177286477	0.0
24	1.9453171353259866E-4	0.0	0.0	0.05928353969905944	0.0
25	1.9453171353259866E-4	0.0	0.0	0.0654112886753363	0.0
26	1.9453171353259866E-4	0.0	0.0	0.07440838042621899	0.0
27	1.9453171353259866E-4	0.0	0.0	0.12522979058661038	0.0
28	1.9453171353259866E-4	0.0	0.0	0.20921885790430986	0.0
29	3.890634270651973E-4	0.0	0.0	0.3077978037369542	0.0
30	3.890634270651973E-4	0.0	0.0	0.5060256198266723	0.0
31	3.890634270651973E-4	0.0	0.0	0.74670998239488	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6960	0.0	28.308187	1
TAATACG	65	2.6845573E-6	22.76923	4
GTATTGG	860	0.0	22.156977	1
ATTGGAC	875	0.0	21.142857	3
TTGGACC	1430	0.0	18.499998	4
GGACCCT	1450	0.0	18.11724	6
TGGACCC	1625	0.0	17.532307	5
GTATCAA	11395	0.0	17.225538	2
TATTGGA	1115	0.0	16.591928	2
ACGTTTA	145	2.9831426E-9	16.586206	26
CGTCTGT	645	0.0	16.348837	34
CGATTAG	105	9.350195E-6	15.857142	1
TTTTTAC	1360	0.0	15.643381	1
CGATCTG	215	0.0	15.488372	33
TAGGGGT	145	5.3516487E-8	15.310345	4
TCTATAC	145	5.3516487E-8	15.310345	3
CGACGAA	255	0.0	15.235294	10
ACGGACC	85	5.366709E-4	15.235293	8
TCTGTCG	100	1.0940306E-4	14.799999	8
ACGATCT	230	1.8189894E-12	14.478261	32
>>END_MODULE