FastQCFastQC Report
Wed 25 May 2016
SRR2088123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973963
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT286180.9622850048907804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT281800.9475571821169262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT227120.7636947736068No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG170860.5745195888449184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117410.39479307577128564No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA90720.3050475073159955No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT88230.2966748409445578No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT80440.2704808365134334No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA72110.24247107311019No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA68300.2296598848069058No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG65860.22145534426621988No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT59880.2013474949083092No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT59720.20080949224990358No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA48970.1646624386382749No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA46000.1546757642916203No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39990.1344670394352586No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT38380.1290533876850519No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35440.11916758883684835No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA33400.11230805494217648No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32530.10938266548709584No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30040.10100999911565813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65350.029.384851
TAGACCG500.00703744318.55
GTCGTAC500.00703744318.51
ATACGTA500.00703744318.56
GTATCAA108500.017.7327182
GTATTGG11850.017.6413521
TAGGCCG752.0686576E-417.2666665
ATTGGAC11500.017.2130433
ACGAACG1251.6601916E-716.27999915
CGAATTA1606.311893E-1016.187515
GGACCCT16250.015.4830776
TTGGACC17550.014.9686614
TTGCGCG750.004106444314.818
TTAGTAC1001.0942907E-414.83
TGGACCC17450.014.736395
GTATTAG5050.014.6534661
TATTGGA15350.014.5830612
ACGGACC2800.014.5357148
TCCGATA1458.922889E-714.0344838
TAACGAA1458.922889E-714.03448313
Produced by FastQC (version 0.11.5)