##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088123_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2973963
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.619281073772605	31.0	31.0	34.0	30.0	34.0
2	31.79947968417899	31.0	31.0	34.0	30.0	34.0
3	31.89698325096849	31.0	31.0	34.0	30.0	34.0
4	35.6046043612513	37.0	35.0	37.0	33.0	37.0
5	35.490822515276754	37.0	35.0	37.0	33.0	37.0
6	35.50395852268505	37.0	35.0	37.0	33.0	37.0
7	35.3745967922264	37.0	35.0	37.0	33.0	37.0
8	35.4127408444557	37.0	35.0	37.0	33.0	37.0
9	36.935712717340465	39.0	37.0	39.0	33.0	39.0
10	36.79292714805127	39.0	37.0	39.0	32.0	39.0
11	36.8590322071929	39.0	37.0	39.0	33.0	39.0
12	36.74774131352677	39.0	35.0	39.0	32.0	39.0
13	36.76024146904316	39.0	35.0	39.0	32.0	39.0
14	37.78893046080264	40.0	37.0	41.0	32.0	41.0
15	37.795609763806745	40.0	37.0	41.0	32.0	41.0
16	37.68268468706571	39.0	37.0	41.0	32.0	41.0
17	37.68155958900632	39.0	37.0	41.0	32.0	41.0
18	37.65551958783616	39.0	37.0	41.0	32.0	41.0
19	37.68846216311366	39.0	37.0	41.0	32.0	41.0
20	37.59972938466282	39.0	37.0	41.0	32.0	41.0
21	37.518641287736266	39.0	36.0	41.0	32.0	41.0
22	37.412003780813684	39.0	36.0	41.0	32.0	41.0
23	37.18904034784562	39.0	36.0	41.0	31.0	41.0
24	37.04350894748859	39.0	36.0	41.0	31.0	41.0
25	36.92948365531111	39.0	36.0	40.0	30.0	41.0
26	37.08183861063503	39.0	36.0	41.0	31.0	41.0
27	36.99193265013721	39.0	36.0	41.0	31.0	41.0
28	36.91150629648049	39.0	36.0	41.0	30.0	41.0
29	36.829958879784314	39.0	35.0	41.0	30.0	41.0
30	36.65212748107491	39.0	35.0	40.0	30.0	41.0
31	36.48692266850664	39.0	35.0	40.0	30.0	41.0
32	36.34173827986427	38.0	35.0	40.0	30.0	41.0
33	36.148202247304354	38.0	35.0	40.0	30.0	41.0
34	36.01852948405881	38.0	35.0	40.0	29.0	41.0
35	35.81745199923469	38.0	35.0	40.0	28.0	41.0
36	35.73750850296389	38.0	35.0	40.0	27.0	41.0
37	35.59295155992189	38.0	34.0	40.0	27.0	41.0
38	35.491815130181514	38.0	34.0	40.0	26.0	41.0
39	35.39204421843849	38.0	34.0	40.0	25.0	41.0
40	35.21671385958736	38.0	34.0	40.0	25.0	41.0
41	35.07360616120644	38.0	34.0	40.0	24.0	41.0
42	34.88710115088856	38.0	34.0	40.0	24.0	41.0
43	34.574883749394324	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	9.0
13	7.0
14	7.0
15	23.0
16	38.0
17	94.0
18	247.0
19	522.0
20	1119.0
21	2153.0
22	3989.0
23	6844.0
24	11363.0
25	17346.0
26	24784.0
27	34997.0
28	47208.0
29	62722.0
30	79481.0
31	99236.0
32	122303.0
33	151518.0
34	186021.0
35	230555.0
36	296563.0
37	403941.0
38	554222.0
39	636647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.62908650847371	22.489149999512435	13.533288746363018	23.348474745650837
2	18.081126093364308	22.563461616704714	35.90865118362266	23.446761106308315
3	19.478621623739098	25.4973582388214	31.930155149879134	23.09386498756037
4	13.431841620087406	15.842160780076956	35.55750357351453	35.168494026321106
5	11.656029345355003	40.19639787045098	34.670774316963595	13.476798467230427
6	32.16832220172208	36.76353740782922	15.325644602841393	15.74249578760731
7	25.81541868543758	34.380790884082955	21.214722577247937	18.58906785323153
8	27.718670339879818	34.555507247400186	19.792613425251087	17.93320898746891
9	25.66662732522227	14.015036501799113	22.35781010052916	37.960526072449454
10	16.237155606845143	28.008351146265102	33.522575768427515	22.23191747846224
11	32.70709823894917	21.977274095205622	24.083520877697538	21.232106788147668
12	19.912823394238597	27.158374196316498	31.52927591903463	21.39952649041027
13	30.780947846358547	20.966266224562983	27.71951096903358	20.533274960044896
14	20.924234766875042	22.197283557327378	27.531378164422353	29.347103511375227
15	23.36437944923995	29.11838513122053	25.54332384094893	21.973911578590588
16	22.491739137306013	28.633611110830902	25.893328195407943	22.981321556455143
17	20.33397860027176	27.30602230088269	27.30514804656278	25.05485105228276
18	21.37498011912051	26.799223796664585	30.439820535763225	21.38597554845168
19	20.9344231922186	25.270690993801875	31.33852035146369	22.456365462515844
20	24.56489875630598	24.60709833982467	30.255756376256194	20.572246527613157
21	23.806348633120184	25.42889740053928	29.196395516689343	21.56835844965119
22	22.579937948118385	24.88857460566927	30.22398059424411	22.30750685196823
23	21.672058462058878	26.45594447543564	31.043257767497444	20.82873929500804
24	20.907960186458272	25.582732535677145	29.6927365942347	23.81657068362989
25	21.81863056130826	26.51623439834322	30.431817746219437	21.23331729412908
26	22.884481077942127	25.77725412185693	29.348616643851994	21.989648156348952
27	21.794521317178457	26.942702380628138	29.04874741212315	22.214028890070253
28	21.653161118682377	26.261860016415806	29.364218720945757	22.720760143956063
29	20.788859847953724	26.201637343840524	30.42630321897078	22.58319958923497
30	20.385828606475602	26.3856678781814	31.678638907074504	21.549864608268496
31	21.396163973795236	25.982804762534034	30.785319117958093	21.83571214571264
32	22.35582621572629	25.95203773550646	29.29142023623024	22.40071581253701
33	23.101867777104154	25.82870062606697	29.02379753883959	22.04563405798929
34	21.766040801449112	27.971195337668963	29.060179968614268	21.202583892267658
35	20.78532920550794	28.51578852864007	29.417850860955568	21.28103140489643
36	21.351005375655312	25.623049109891415	31.05909522075426	21.966850293699014
37	21.525688113806392	25.807752147555302	30.470486687292343	22.196073051345966
38	21.49972948553832	25.320254488707494	31.35993958230146	21.820076443452727
39	22.305892843993018	25.009759704475137	31.242386001439833	21.441961450092016
40	21.096092991069494	25.429704404526888	30.532928620833548	22.94127398357007
41	21.612743668969657	25.22058949623785	30.503405724953538	22.66326110983896
42	19.88282974603248	26.513880636712695	30.8581176026736	22.745172014581215
43	20.39890879610809	26.216667793109732	30.43195224688404	22.95247116389814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	208.0
1	320.0
2	432.0
3	834.0
4	1236.0
5	1236.0
6	2231.0
7	3226.0
8	3707.0
9	4188.0
10	6746.5
11	9305.0
12	9305.0
13	18229.5
14	27154.0
15	41982.5
16	56811.0
17	54704.5
18	52598.0
19	52598.0
20	58717.5
21	64837.0
22	54663.5
23	44490.0
24	50500.5
25	56511.0
26	56511.0
27	63211.5
28	69912.0
29	75519.5
30	81127.0
31	88295.5
32	95464.0
33	95464.0
34	105447.5
35	115431.0
36	125481.0
37	135531.0
38	141817.5
39	148104.0
40	148104.0
41	155769.5
42	163435.0
43	179855.0
44	196275.0
45	238632.5
46	280990.0
47	280990.0
48	282640.5
49	284291.0
50	261208.0
51	238125.0
52	226262.0
53	214399.0
54	214399.0
55	189479.0
56	164559.0
57	142701.5
58	120844.0
59	108422.5
60	96001.0
61	96001.0
62	85225.5
63	74450.0
64	65119.0
65	55788.0
66	47784.5
67	39781.0
68	39781.0
69	33944.5
70	28108.0
71	24278.5
72	20449.0
73	16603.5
74	12758.0
75	12758.0
76	9995.5
77	7233.0
78	5632.0
79	4031.0
80	3255.0
81	2479.0
82	2479.0
83	1939.0
84	1399.0
85	1085.5
86	772.0
87	643.0
88	514.0
89	514.0
90	479.0
91	444.0
92	286.5
93	129.0
94	110.0
95	91.0
96	91.0
97	58.0
98	25.0
99	26.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2973963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.10027616042369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2054185641579	34.669905645679336
2	13.276585134220223	12.241084823098564
3	4.78452030341631	6.617031218479383
4	2.2000155622557274	4.056852999088753
5	1.2087462853001032	2.7861768780110516
6	0.7017692000455882	1.9411052353788742
7	0.46446501383694627	1.4988375783316763
8	0.3286486737190804	1.2120635694565272
9	0.24444293846552687	1.014199827185362
>10	1.37572432364968	11.880465259169922
>50	0.12210931337838855	3.8758303072535756
>100	0.07643756239839121	6.687041710378863
>500	0.005339145634209033	1.6601373389825764
>1k	0.004827172765140638	4.233102392430405
>5k	5.851118503200774E-4	1.9733370818847278
>10k+	3.6569490645004833E-4	3.6528281351902705
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28618	0.9622850048907804	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28180	0.9475571821169262	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22712	0.7636947736068	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	17086	0.5745195888449184	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11741	0.39479307577128564	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9072	0.3050475073159955	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8823	0.2966748409445578	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8044	0.2704808365134334	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7211	0.24247107311019	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6830	0.2296598848069058	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6586	0.22145534426621988	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5988	0.2013474949083092	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5972	0.20080949224990358	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4897	0.1646624386382749	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4600	0.1546757642916203	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3999	0.1344670394352586	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3838	0.1290533876850519	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3544	0.11916758883684835	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3340	0.11230805494217648	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3253	0.10938266548709584	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3004	0.10100999911565813	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	6.725033230070449E-5	0.0	0.0	0.0	0.0
7	6.725033230070449E-5	0.0	0.0	0.0	0.0
8	6.725033230070449E-5	0.0	0.0	0.0	0.0
9	6.725033230070449E-5	0.0	0.0	3.362516615035224E-5	0.0
10	6.725033230070449E-5	0.0	0.0	1.681258307517612E-4	0.0
11	6.725033230070449E-5	0.0	0.0	3.0262649535317017E-4	0.0
12	6.725033230070449E-5	0.0	0.0	5.380026584056359E-4	0.0
13	6.725033230070449E-5	0.0	0.0	7.061284891573971E-4	0.0
14	6.725033230070449E-5	0.0	0.0	8.40629153758806E-4	0.0
15	6.725033230070449E-5	0.0	0.0	0.0016476331413672598	0.0
16	6.725033230070449E-5	0.0	0.0	0.00255551262742677	0.0
17	6.725033230070449E-5	0.0	0.0	0.0037996437749898034	0.0
18	6.725033230070449E-5	0.0	0.0	0.004304021267245087	0.0
19	1.0087549845105673E-4	0.0	0.0	0.004976524590252131	0.0
20	1.0087549845105673E-4	0.0	0.0	0.007027659725423618	0.0
21	1.0087549845105673E-4	0.0	0.0	0.009684047851301445	0.0
22	1.0087549845105673E-4	0.0	0.0	0.017585961896634222	0.0
23	1.0087549845105673E-4	0.0	0.0	0.027135509083334258	0.0
24	1.0087549845105673E-4	0.0	0.0	0.04142620469723396	0.0
25	1.0087549845105673E-4	0.0	0.0	0.0472433584412449	0.0
26	1.0087549845105673E-4	0.0	0.0	0.053934766505164995	0.0
27	1.0087549845105673E-4	0.0	0.0	0.09690772884531516	0.0
28	1.0087549845105673E-4	0.0	0.0	0.17125297120374397	0.0
29	1.0087549845105673E-4	0.0	0.0	0.25941815684996755	0.0
30	1.0087549845105673E-4	0.0	0.0	0.42034820204555334	0.0
31	1.0087549845105673E-4	0.0	0.0	0.632422124955825	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6535	0.0	29.38485	1
TAGACCG	50	0.007037443	18.5	5
GTCGTAC	50	0.007037443	18.5	1
ATACGTA	50	0.007037443	18.5	6
GTATCAA	10850	0.0	17.732718	2
GTATTGG	1185	0.0	17.641352	1
TAGGCCG	75	2.0686576E-4	17.266666	5
ATTGGAC	1150	0.0	17.213043	3
ACGAACG	125	1.6601916E-7	16.279999	15
CGAATTA	160	6.311893E-10	16.1875	15
GGACCCT	1625	0.0	15.483077	6
TTGGACC	1755	0.0	14.968661	4
TTGCGCG	75	0.0041064443	14.8	18
TTAGTAC	100	1.0942907E-4	14.8	3
TGGACCC	1745	0.0	14.73639	5
GTATTAG	505	0.0	14.653466	1
TATTGGA	1535	0.0	14.583061	2
ACGGACC	280	0.0	14.535714	8
TCCGATA	145	8.922889E-7	14.034483	8
TAACGAA	145	8.922889E-7	14.034483	13
>>END_MODULE