FastQCFastQC Report
Wed 25 May 2016
SRR2088122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547696
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69941.2769857731296193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67851.238825918027519No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54771.0000073033215506No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG39920.7288714907539949No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28700.5240133212585083No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21030.38397213052496276No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT20900.3815985510210044No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA19310.35256784785720546No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT19250.35147234962460927No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA16590.30290526131284506No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG16040.2928631941807134No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT15330.2798997984283252No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT14710.2685796500248313No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA12920.23589728608571178No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA12050.2200125617130671No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA10020.18294820484356283No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT9960.18185270661096667No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.16341181969559757No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC8270.15099617305950747No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA8120.14825742747801698No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.13237270310537233No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT7240.1321901200666063No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG6980.1274429610586895No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA6850.12506938155473107No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.1221480529344746No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC6690.1221480529344746No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG6500.11867897519792002No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA6170.112653734918641No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG5890.10754140983319214No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC5640.10297683386404136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGT250.005493861629.66
GGTATCA15400.027.8701291
AAAGCCG358.8634435E-426.4285719
GTATTGG2250.025.488891
ATTGGAC2400.024.6666663
ACCGACC400.001929902923.1250028
CTGACCT502.699599E-422.24
GTACTAG603.7217033E-521.5833321
GTATACG603.7217033E-521.5833321
TTGGACC3250.021.0615394
TACTAGG450.003823289220.5555552
TATAGGT1001.2860255E-820.354
ATAGCAC656.8937E-519.9230773
TAGGCAT656.8937E-519.9230775
TATTGGA3000.019.7333322
GGACCTT759.251256E-619.7333326
CTTCTAT1103.8406142E-818.51
ATACGGG500.007030293318.53
GACCTTC500.007030293318.57
GGTTATC500.007030293318.52
Produced by FastQC (version 0.11.5)