##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088122_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	547696
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.661542169378635	31.0	31.0	34.0	30.0	34.0
2	31.834094095994857	31.0	31.0	34.0	30.0	34.0
3	31.93032083491572	31.0	31.0	34.0	30.0	34.0
4	35.62984210218808	37.0	35.0	37.0	33.0	37.0
5	35.54175491513541	37.0	35.0	37.0	33.0	37.0
6	35.56299845169583	37.0	35.0	37.0	33.0	37.0
7	35.4201290496918	37.0	35.0	37.0	33.0	37.0
8	35.47897008559493	37.0	35.0	37.0	33.0	37.0
9	36.99048012035874	39.0	37.0	39.0	33.0	39.0
10	36.86899119219421	39.0	37.0	39.0	33.0	39.0
11	36.93693034091905	39.0	37.0	39.0	33.0	39.0
12	36.83021420642108	39.0	37.0	39.0	32.0	39.0
13	36.82289810405773	39.0	37.0	39.0	32.0	39.0
14	37.82714315970904	40.0	37.0	41.0	32.0	41.0
15	37.83502526949257	40.0	37.0	41.0	32.0	41.0
16	37.72541884549093	40.0	37.0	41.0	32.0	41.0
17	37.7624430340919	39.0	37.0	41.0	32.0	41.0
18	37.75271318395606	39.0	37.0	41.0	32.0	41.0
19	37.80294908124215	40.0	37.0	41.0	32.0	41.0
20	37.710405042213196	39.0	37.0	41.0	32.0	41.0
21	37.63303730536648	39.0	37.0	41.0	32.0	41.0
22	37.5483242528702	39.0	36.0	41.0	32.0	41.0
23	37.31262415646636	39.0	36.0	41.0	31.0	41.0
24	37.16222320411323	39.0	36.0	41.0	31.0	41.0
25	37.0429983056294	39.0	36.0	41.0	31.0	41.0
26	37.18833988197832	39.0	36.0	41.0	31.0	41.0
27	37.1070520872893	39.0	36.0	41.0	31.0	41.0
28	37.0230529344746	39.0	36.0	41.0	31.0	41.0
29	36.929097528555985	39.0	35.0	41.0	31.0	41.0
30	36.73133453594695	39.0	35.0	40.0	30.0	41.0
31	36.531057374894104	39.0	35.0	40.0	30.0	41.0
32	36.36754878618796	38.0	35.0	40.0	30.0	41.0
33	36.1655279571149	38.0	35.0	40.0	30.0	41.0
34	36.0181998773042	38.0	35.0	40.0	30.0	41.0
35	35.80479499576408	38.0	35.0	40.0	28.0	41.0
36	35.70845688118956	38.0	35.0	40.0	27.0	41.0
37	35.526788583447754	38.0	34.0	40.0	27.0	41.0
38	35.395319666384275	38.0	34.0	40.0	26.0	41.0
39	35.26823091642079	38.0	34.0	40.0	25.0	41.0
40	35.051008223540066	38.0	34.0	40.0	24.0	41.0
41	34.88079883731121	38.0	34.0	40.0	24.0	41.0
42	34.642560836668515	38.0	33.0	40.0	23.0	41.0
43	34.317464432824046	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	1.0
15	2.0
16	5.0
17	12.0
18	38.0
19	74.0
20	185.0
21	355.0
22	659.0
23	1146.0
24	1903.0
25	2994.0
26	4340.0
27	6165.0
28	8522.0
29	11303.0
30	14186.0
31	17924.0
32	22478.0
33	27853.0
34	34762.0
35	42990.0
36	55412.0
37	77715.0
38	101376.0
39	115294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.66672022435804	22.825801174374106	13.971984458531741	22.535494142736116
2	17.91322193333528	22.66092869036838	36.275232975957465	23.150616400338876
3	19.748729222050187	25.67172299962023	32.25329379801934	22.326253980310245
4	13.114209342408929	15.562647892261399	35.0506485349537	36.272494230375976
5	11.055220414244399	41.068950658759604	34.273940288043	13.601888638952996
6	31.87680757208378	36.208407583769095	15.249700563816424	16.665084280330696
7	25.121600303818177	35.20803511437002	20.840575793871054	18.829788787940753
8	28.307674330285415	33.86112003739301	19.632423826356227	18.198781805965353
9	25.621512663959567	13.788853670649411	23.192062750138764	37.39757091525225
10	16.670561771493674	28.148096754403902	32.777489702316615	22.403851771785806
11	31.778943063305192	21.76061172621308	24.63903333235956	21.82141187812217
12	19.9566913032047	27.66881627764307	31.471290642984428	20.9032017761678
13	31.352246501708976	21.042512634746284	28.244135432794838	19.361105430749905
14	20.710028921153338	22.556308609155444	27.87276153194473	28.860900937746486
15	23.262357220063684	28.981040577254536	26.408628144079927	21.347974058601853
16	22.011298238438844	28.622447489118052	26.436380765972363	22.929873506470745
17	19.606314451812683	27.168173585346615	27.490067482691128	25.73544448014957
18	20.664200578423067	26.711168239315242	31.67450556513102	20.95012561713067
19	20.138726592854432	24.803905816365283	32.88265753264585	22.17471005813444
20	24.843708918816276	23.858125675557243	31.414872484005723	19.883292921620754
21	23.712424410621953	24.995252840992084	30.23538605357717	21.056936694808797
22	22.207940171189858	24.335397738891647	31.460883409774766	21.99577868014373
23	21.112441938593673	26.476001285384594	32.30350413368	20.108052642341736
24	19.964724956910405	25.595038123338494	30.678697671701087	23.761539248050013
25	21.06387485028191	26.57879553620987	31.79902719756946	20.55830241593877
26	22.700184043703075	25.448789109286903	30.275371739066927	21.575655107943092
27	21.195152055154683	27.114859338026935	29.846849347083054	21.843139259735327
28	20.92858081855628	26.314780462154186	30.30604568957962	22.45059302970991
29	19.944275656568607	26.259457801408082	31.37981654056265	22.416450001460664
30	19.264519003242675	26.39365633490111	33.34459262072391	20.997232041132307
31	20.743441617247523	26.00950162133738	32.12092109491397	21.126135666501124
32	22.084331453945254	26.076327013525752	30.02048581694955	21.818855715579446
33	22.940098156641643	25.956187374017702	29.64911191609944	21.454602553241216
34	21.063509684204377	28.75865443603751	29.722145131605853	20.45569074815226
35	19.691945896993953	29.57553095147673	30.019207735678187	20.71331541585113
36	20.60613917209547	25.660585434255502	32.437154918056734	21.2961204755923
37	20.726278811603517	26.10316672022436	31.490279279016097	21.680275189156028
38	20.912513511144866	25.52364815518098	32.43587683678537	21.127961496888787
39	21.939543104203793	25.165785399199557	32.241060734422014	20.653610762174637
40	20.260509479711374	25.842072974788934	31.31591247699454	22.581505068505155
41	21.22107884665946	25.321163565189448	31.129677777453185	22.328079810697908
42	18.70891881627764	27.22641757471298	31.623199731237765	22.44146387777161
43	19.58805614793608	26.848653267506062	31.114523385235604	22.44876719932225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	35.0
1	62.0
2	89.0
3	186.0
4	283.0
5	283.0
6	536.0
7	789.0
8	869.0
9	949.0
10	1566.5
11	2184.0
12	2184.0
13	4275.5
14	6367.0
15	10183.0
16	13999.0
17	12980.5
18	11962.0
19	11962.0
20	12961.5
21	13961.0
22	11067.0
23	8173.0
24	9434.0
25	10695.0
26	10695.0
27	11584.0
28	12473.0
29	13013.5
30	13554.0
31	14714.0
32	15874.0
33	15874.0
34	17605.5
35	19337.0
36	21211.5
37	23086.0
38	24267.0
39	25448.0
40	25448.0
41	27014.0
42	28580.0
43	32647.5
44	36715.0
45	46520.5
46	56326.0
47	56326.0
48	57001.0
49	57676.0
50	51912.0
51	46148.0
52	43404.5
53	40661.0
54	40661.0
55	35445.0
56	30229.0
57	25015.5
58	19802.0
59	17413.0
60	15024.0
61	15024.0
62	13243.5
63	11463.0
64	10082.5
65	8702.0
66	7404.0
67	6106.0
68	6106.0
69	5268.5
70	4431.0
71	3572.5
72	2714.0
73	2204.0
74	1694.0
75	1694.0
76	1307.0
77	920.0
78	727.5
79	535.0
80	414.5
81	294.0
82	294.0
83	241.5
84	189.0
85	143.5
86	98.0
87	72.0
88	46.0
89	46.0
90	50.0
91	54.0
92	36.0
93	18.0
94	14.5
95	11.0
96	11.0
97	5.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	547696.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.46946628859915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.86298603307925	48.77030514977908
2	8.976861314287305	10.317908573577283
3	2.427144900499556	4.184601661104137
4	1.0432113869771227	2.3981120654305808
5	0.5902995667650001	1.6962100526187924
6	0.3873368401370717	1.3356024885953974
7	0.26913559915468227	1.0826955469877362
8	0.19955051700704532	0.9174449368007136
9	0.1493323510257354	0.7723845461763678
>10	0.9729729543799868	10.218612475224447
>50	0.06710383572801701	2.685787468293354
>100	0.04388781607278107	5.5530642880819725
>500	0.005088442443220991	2.0376892916376224
>1k	0.004134359485117055	4.510185545765685
>5k	9.540829581039357E-4	3.5193959099268155
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6994	1.2769857731296193	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6785	1.238825918027519	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5477	1.0000073033215506	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3992	0.7288714907539949	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2870	0.5240133212585083	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2103	0.38397213052496276	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2090	0.3815985510210044	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1931	0.35256784785720546	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1925	0.35147234962460927	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1659	0.30290526131284506	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1604	0.2928631941807134	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1533	0.2798997984283252	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1471	0.2685796500248313	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1292	0.23589728608571178	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1205	0.2200125617130671	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1002	0.18294820484356283	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	996	0.18185270661096667	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	895	0.16341181969559757	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	827	0.15099617305950747	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	812	0.14825742747801698	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	725	0.13237270310537233	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	724	0.1321901200666063	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	698	0.1274429610586895	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	685	0.12506938155473107	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	669	0.1221480529344746	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	669	0.1221480529344746	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	650	0.11867897519792002	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	617	0.112653734918641	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	589	0.10754140983319214	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	564	0.10297683386404136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.651660775320616E-4	0.0
11	0.0	0.0	0.0	5.477491162980924E-4	0.0
12	0.0	0.0	0.0	5.477491162980924E-4	0.0
13	0.0	0.0	0.0	7.303321550641232E-4	0.0
14	0.0	0.0	0.0	7.303321550641232E-4	0.0
15	0.0	0.0	0.0	0.0016432473488942772	0.0
16	0.0	0.0	0.0	0.002738745581490462	0.0
17	0.0	0.0	0.0	0.005112325085448862	0.0
18	0.0	0.0	0.0	0.006390406356811078	0.0
19	0.0	0.0	0.0	0.006755572434343139	0.0
20	0.0	0.0	0.0	0.009859484093365662	0.0
21	0.0	0.0	0.0	0.014424060062516432	0.0
22	0.0	0.0	0.0	0.026291957582308435	0.0
23	0.0	0.0	0.0	0.03870760421839853	0.0
24	0.0	0.0	0.0	0.0598872367152581	0.0
25	0.0	0.0	0.0	0.0688338056147936	0.0
26	0.0	0.0	0.0	0.07869328970815927	0.0
27	0.0	0.0	0.0	0.12890362536881775	0.0
28	0.0	0.0	0.0	0.19956326137127164	0.0
29	0.0	0.0	0.0	0.2857424556688382	0.0
30	0.0	0.0	0.0	0.47088165697759343	0.0
31	0.0	0.0	0.0	0.6848689784113815	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGCGT	25	0.0054938616	29.6	6
GGTATCA	1540	0.0	27.870129	1
AAAGCCG	35	8.8634435E-4	26.42857	19
GTATTGG	225	0.0	25.48889	1
ATTGGAC	240	0.0	24.666666	3
ACCGACC	40	0.0019299029	23.125002	8
CTGACCT	50	2.699599E-4	22.2	4
GTACTAG	60	3.7217033E-5	21.583332	1
GTATACG	60	3.7217033E-5	21.583332	1
TTGGACC	325	0.0	21.061539	4
TACTAGG	45	0.0038232892	20.555555	2
TATAGGT	100	1.2860255E-8	20.35	4
ATAGCAC	65	6.8937E-5	19.923077	3
TAGGCAT	65	6.8937E-5	19.923077	5
TATTGGA	300	0.0	19.733332	2
GGACCTT	75	9.251256E-6	19.733332	6
CTTCTAT	110	3.8406142E-8	18.5	1
ATACGGG	50	0.0070302933	18.5	3
GACCTTC	50	0.0070302933	18.5	7
GGTTATC	50	0.0070302933	18.5	2
>>END_MODULE