FastQCFastQC Report
Wed 25 May 2016
SRR2088121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677677
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70621.042089372960865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69611.0271855175843358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57860.8537990812732319No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG45560.6722966841135232No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29820.4400326409189038No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA24000.3541510188482123No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT22880.3376239713019624No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT21550.3179981023407907No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA19970.2946831602666167No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG18030.2660559529097195No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA17880.2638425090419182No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT15840.23373967243982013No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT15280.22547614866669521No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA13210.1949306232910369No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA12350.18224021178230929No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA11500.16969736319810175No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT10380.15317031565185182No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC10050.1483007391426889No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA9230.13620057933204166No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG8210.12114916103099264No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.11952596886127168No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC7930.11701739914443017No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT7760.11450882942758867No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.11155757093718689No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG7350.10845874952226503No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC7140.10535992810734318No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC7090.10462211348474272No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.10299892131502175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.029.3987921
GATTCGC358.8656234E-426.4285710
TCTAAGC659.379983E-825.6153853
GTATTGG3050.024.2622951
TAACGCT551.9011646E-523.54545432
TTACGTT551.9011646E-523.54545418
CTGTGCG502.7005165E-422.29
ATTGGAC3200.020.81253
TGCGACG450.003824212120.55555522
CAATGCG450.003824212120.55555519
CTTAACG656.896789E-519.92307730
ATTACGT656.896789E-519.92307717
GTACTAG656.896789E-519.9230771
TATTGGA3900.018.974362
TGGACCC4400.018.9204545
TTGGACC4500.018.9111124
CCGTGCT500.00703197518.59
CGCGCGC609.2314853E-418.522
GTCTATG609.2314853E-418.51
CGATTCG500.00703197518.59
Produced by FastQC (version 0.11.5)