##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088121_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	677677
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66315663656875	31.0	31.0	34.0	30.0	34.0
2	31.847195640400958	31.0	31.0	34.0	30.0	34.0
3	31.950346551528234	31.0	31.0	34.0	30.0	34.0
4	35.64453862238205	37.0	35.0	37.0	33.0	37.0
5	35.5479572126544	37.0	35.0	37.0	33.0	37.0
6	35.56185321325646	37.0	35.0	37.0	33.0	37.0
7	35.429555673277974	37.0	35.0	37.0	33.0	37.0
8	35.477225876044194	37.0	35.0	37.0	33.0	37.0
9	36.99419192329089	39.0	37.0	39.0	33.0	39.0
10	36.867234685550784	39.0	37.0	39.0	33.0	39.0
11	36.922522086480726	39.0	37.0	39.0	33.0	39.0
12	36.8100097834219	39.0	37.0	39.0	32.0	39.0
13	36.82366673208623	39.0	37.0	39.0	32.0	39.0
14	37.84926152134424	40.0	37.0	41.0	32.0	41.0
15	37.85389057028643	40.0	37.0	41.0	32.0	41.0
16	37.742859798989784	40.0	37.0	41.0	32.0	41.0
17	37.752286118608126	40.0	37.0	41.0	32.0	41.0
18	37.74067881896538	39.0	37.0	41.0	32.0	41.0
19	37.793234830162454	40.0	37.0	41.0	32.0	41.0
20	37.69367117373026	39.0	37.0	41.0	32.0	41.0
21	37.61374076440546	39.0	37.0	41.0	32.0	41.0
22	37.522384557835075	39.0	36.0	41.0	32.0	41.0
23	37.28223327632486	39.0	36.0	41.0	31.0	41.0
24	37.154685786886674	39.0	36.0	41.0	31.0	41.0
25	37.042326949269345	39.0	36.0	41.0	31.0	41.0
26	37.194942428325	39.0	36.0	41.0	31.0	41.0
27	37.110979124273065	39.0	36.0	41.0	31.0	41.0
28	37.048866938084075	39.0	36.0	41.0	31.0	41.0
29	36.961834325202126	39.0	36.0	41.0	31.0	41.0
30	36.77562614637947	39.0	35.0	41.0	30.0	41.0
31	36.602134940391956	39.0	35.0	40.0	30.0	41.0
32	36.44953864451649	38.0	35.0	40.0	30.0	41.0
33	36.244290421543006	38.0	35.0	40.0	30.0	41.0
34	36.12043348084707	38.0	35.0	40.0	30.0	41.0
35	35.9070014180797	38.0	35.0	40.0	29.0	41.0
36	35.82180891486652	38.0	35.0	40.0	28.0	41.0
37	35.667471376481714	38.0	35.0	40.0	27.0	41.0
38	35.56096045756312	38.0	35.0	40.0	27.0	41.0
39	35.46042288582909	38.0	34.0	40.0	26.0	41.0
40	35.26714496729268	38.0	34.0	40.0	25.0	41.0
41	35.12094257293666	38.0	34.0	40.0	24.0	41.0
42	34.90931225347769	38.0	34.0	40.0	24.0	41.0
43	34.597696247622395	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	2.0
15	5.0
16	9.0
17	25.0
18	40.0
19	103.0
20	227.0
21	391.0
22	759.0
23	1376.0
24	2282.0
25	3681.0
26	5291.0
27	7531.0
28	10228.0
29	13593.0
30	17359.0
31	21589.0
32	27407.0
33	34284.0
34	42178.0
35	52073.0
36	68250.0
37	94811.0
38	127297.0
39	146885.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.666866368491185	22.69665342043481	13.686608812162726	22.94987139891128
2	17.821174394291088	22.67865074364336	36.28882196090468	23.21135290116088
3	19.4123454093912	25.886521159785563	32.08165541991244	22.619478010910804
4	13.102259631063177	15.684020558466644	35.90073146941685	35.312988341053334
5	11.284136838051166	40.60341431094754	34.82042329900528	13.292025551996009
6	31.681612331538478	36.898256839172646	15.42726106980169	15.992869759487188
7	25.459178930375387	34.64836492901486	21.343796528434638	18.548659612175122
8	27.803953800999594	34.23651680667929	19.992120139830625	17.967409252490494
9	25.56689986527505	13.989703059126986	22.86487515438771	37.57852192121025
10	16.333002300505996	27.87286568675047	33.56436768549029	22.22976432725325
11	32.097592215760606	22.107287099901576	24.487624635335123	21.307496049002697
12	19.8060432920108	27.441834384227292	31.778266047099134	20.973856276662776
13	30.874295571489075	21.13130591712571	28.003163749101713	19.991234762283508
14	20.708833264224698	22.542745290160358	27.86961930241103	28.878802143203913
15	23.139194631070552	29.30156992195397	25.98538831921402	21.573847127761457
16	22.17974049584094	28.77108120830425	26.220456057974523	22.82872223788029
17	19.74982181776864	27.394466685456347	27.446703960736457	25.40900753603855
18	20.880596508366082	27.047841375758658	31.10493642251397	20.96662569336129
19	20.390097347261303	25.221012370199965	32.04225611906557	22.34663416347316
20	24.586934483537142	24.54709249391672	30.800661672153545	20.06531135039259
21	23.68709576981364	25.364148406984448	29.687004280800437	21.26175154240147
22	22.157015805464845	24.849596489182897	30.78944688989002	22.203940815462232
23	21.338189137302873	26.718185802380777	31.71658474464974	20.22704031566661
24	20.287541114719847	25.733941095831792	30.16894479228305	23.809572997165315
25	21.32048158636046	26.75478140766176	31.162190837227765	20.76254616875001
26	22.673338478360634	25.8111165053558	29.885623977204478	21.62992103907909
27	21.227074255139247	27.274645590746033	29.526898507696142	21.971381646418575
28	21.31457906937966	26.409631727209277	29.711204600421738	22.564584602989328
29	20.335351502264352	26.37141292975857	30.885215227903558	22.408020340073513
30	19.69669916494141	26.729253021719785	32.3950790715931	21.1789687417457
31	21.122747267503545	26.19478010910803	31.459530130135743	21.222942493252685
32	21.9421641873636	26.257789477878102	29.670624796178714	22.12942153857959
33	22.83078811882357	26.248345450708822	29.42419471223016	21.49667171823745
34	21.23046820240321	28.5239133097331	29.44175470024805	20.803863787615633
35	20.02915843388517	29.40368346572187	29.792364208907784	20.774793891485178
36	20.96116586515405	25.993799405911666	31.653870501728697	21.391164227205586
37	20.973856276662776	26.40063038881355	30.908530169977734	21.71698316454594
38	21.028159432886167	25.91824718855738	31.78416856407994	21.269424814476512
39	22.0134370799068	25.42804315330165	31.70064794880157	20.857871817989988
40	20.543415225837677	25.890210232898564	30.891265307808887	22.675109233454876
41	21.473799464936835	25.595822198480988	30.79047983036166	22.139898506220515
42	19.211954957892917	27.14538046886644	31.091065507609084	22.55159906563156
43	19.901516504175294	26.91754331340742	30.62860330216313	22.552336880254163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	30.0
1	58.5
2	87.0
3	190.5
4	294.0
5	294.0
6	571.0
7	848.0
8	981.0
9	1114.0
10	1721.5
11	2329.0
12	2329.0
13	4551.5
14	6774.0
15	10518.0
16	14262.0
17	13765.5
18	13269.0
19	13269.0
20	14478.0
21	15687.0
22	13187.0
23	10687.0
24	12012.5
25	13338.0
26	13338.0
27	14874.5
28	16411.0
29	17610.5
30	18810.0
31	20342.5
32	21875.0
33	21875.0
34	24028.0
35	26181.0
36	28771.5
37	31362.0
38	32417.0
39	33472.0
40	33472.0
41	35410.0
42	37348.0
43	41573.0
44	45798.0
45	55985.5
46	66173.0
47	66173.0
48	66748.5
49	67324.0
50	61471.5
51	55619.0
52	52346.5
53	49074.0
54	49074.0
55	43146.5
56	37219.0
57	31273.0
58	25327.0
59	22460.0
60	19593.0
61	19593.0
62	17319.5
63	15046.0
64	13060.5
65	11075.0
66	9379.5
67	7684.0
68	7684.0
69	6448.0
70	5212.0
71	4305.0
72	3398.0
73	2727.5
74	2057.0
75	2057.0
76	1608.0
77	1159.0
78	951.0
79	743.0
80	576.0
81	409.0
82	409.0
83	331.5
84	254.0
85	190.5
86	127.0
87	111.5
88	96.0
89	96.0
90	81.0
91	66.0
92	45.0
93	24.0
94	20.0
95	16.0
96	16.0
97	9.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	677677.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.86533386689554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.77862929440823	49.90522318193111
2	9.236117088762025	10.87374232127432
3	2.4397327100705084	4.308470415728589
4	0.9788332546550096	2.3047738534114846
5	0.5385448009926338	1.5850809756356103
6	0.3579887199302222	1.2643875311565054
7	0.24501506971496662	1.0096025716834536
8	0.1976134318883175	0.9306064515751066
9	0.15231422450601106	0.8069424910401259
>10	0.9592902509809302	10.44133930049018
>50	0.06915039733639093	2.8132751224776458
>100	0.037969807903100325	4.790970668819666
>500	0.00427474658510376	1.7660293215188152
>1k	0.003771835222150376	4.267423127934578
>5k	7.543670444300752E-4	2.9321326653227904
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7062	1.042089372960865	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6961	1.0271855175843358	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5786	0.8537990812732319	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4556	0.6722966841135232	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2982	0.4400326409189038	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2400	0.3541510188482123	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2288	0.3376239713019624	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2155	0.3179981023407907	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1997	0.2946831602666167	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1803	0.2660559529097195	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1788	0.2638425090419182	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1584	0.23373967243982013	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1528	0.22547614866669521	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1321	0.1949306232910369	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1235	0.18224021178230929	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1150	0.16969736319810175	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1038	0.15317031565185182	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1005	0.1483007391426889	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	923	0.13620057933204166	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	821	0.12114916103099264	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	810	0.11952596886127168	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	793	0.11701739914443017	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	776	0.11450882942758867	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	756	0.11155757093718689	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	735	0.10845874952226503	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	714	0.10535992810734318	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	709	0.10462211348474272	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	698	0.10299892131502175	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.4756292452008848E-4	0.0
11	0.0	0.0	0.0	2.9512584904017695E-4	0.0
12	0.0	0.0	0.0	5.902516980803539E-4	0.0
13	0.0	0.0	0.0	7.378146226004424E-4	0.0
14	0.0	0.0	0.0	7.378146226004424E-4	0.0
15	0.0	0.0	0.0	0.0011805033961607078	0.0
16	0.0	0.0	0.0	0.002803695565881681	0.0
17	0.0	0.0	0.0	0.005164702358203097	0.0
18	0.0	0.0	0.0	0.005754954056283451	0.0
19	0.0	0.0	0.0	0.0069354574524441584	0.0
20	0.0	0.0	0.0	0.00973915301832584	0.0
21	0.0	0.0	0.0	0.01372335198036823	0.0
22	0.0	0.0	0.0	0.022134438678013273	0.0
23	0.0	0.0	0.0	0.03393947263962035	0.0
24	0.0	0.0	0.0	0.050909208959430526	0.0
25	0.0	0.0	0.0	0.05813979226091486	0.0
26	0.0	0.0	0.0	0.06581306433595946	0.0
27	0.0	0.0	0.0	0.10270379546598159	0.0
28	0.0	0.0	0.0	0.1719108070659031	0.0
29	0.0	0.0	0.0	0.25705461451399414	0.0
30	0.0	0.0	0.0	0.41583232129760933	0.0
31	0.0	0.0	0.0	0.6082543748718047	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1655	0.0	29.398792	1
GATTCGC	35	8.8656234E-4	26.42857	10
TCTAAGC	65	9.379983E-8	25.615385	3
GTATTGG	305	0.0	24.262295	1
TAACGCT	55	1.9011646E-5	23.545454	32
TTACGTT	55	1.9011646E-5	23.545454	18
CTGTGCG	50	2.7005165E-4	22.2	9
ATTGGAC	320	0.0	20.8125	3
TGCGACG	45	0.0038242121	20.555555	22
CAATGCG	45	0.0038242121	20.555555	19
CTTAACG	65	6.896789E-5	19.923077	30
ATTACGT	65	6.896789E-5	19.923077	17
GTACTAG	65	6.896789E-5	19.923077	1
TATTGGA	390	0.0	18.97436	2
TGGACCC	440	0.0	18.920454	5
TTGGACC	450	0.0	18.911112	4
CCGTGCT	50	0.007031975	18.5	9
CGCGCGC	60	9.2314853E-4	18.5	22
GTCTATG	60	9.2314853E-4	18.5	1
CGATTCG	50	0.007031975	18.5	9
>>END_MODULE