FastQCFastQC Report
Wed 25 May 2016
SRR2088120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454319
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124800.5084913574804254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT121250.49402706005209596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT97430.3969736615329955No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG61170.249234105265045No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57140.2328140718464063No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34680.14130192529984895No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT33570.13677928582225862No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT29780.12133712039877458No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA28960.11799607141532947No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG25630.1044281529825585No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA25010.10190199399507563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30300.026.3762381
CGAACGC500.007037101318.530
GTATTGG7850.017.9108281
ATTGGAC7200.015.9305553
GTATCAA51100.015.6037182
GGACCCT10150.014.2167496
CGTAGCA1501.307113E-613.5666672
TTGGACC10700.013.4859814
GTACTAG1805.1661118E-813.3611111
CGTATAC850.009408710513.0588233
TATACCG1703.7360223E-713.0588235
TAGAGTG3300.012.8939395
ACCGACC2305.7661964E-1012.8695658
TACGCTG1153.5797444E-412.8695655
GTCTAGA2602.3646862E-1112.8076931
GCGTTAT1901.0473559E-712.6578951
TGGACCC10550.012.6255935
TATTGGA11050.012.5565612
GCCCTAC3150.012.3333341
TTAGAGT2859.094947E-1212.3333334
Produced by FastQC (version 0.11.5)